PRKAG3 ORF clone pFN21AE5658

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE5658

How to Order Order Information

Clone info.

Species

human

Clone Name

pFN21AE5658 Sequence (with vector) Restriction Map

Product ID

FHC16543

ORF Sequence

DNA sequence (1467 bp)
Protein sequence (489 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5658

Symbol

PRKAG3

Alias : AMPKG3, SMGMQTL

Description

protein kinase, AMP-activated, gamma 3 non-catalytic subunit

Original Clone ID

oc95658

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1467 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 489 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2424.1 1.1e-160 99.8 PRKAG3 CCDS47752.1 5.6e-65 63.0 PRKAG2 CCDS43683.1 8.3e-65 63.0 PRKAG2 CCDS5928.1 8.8e-65 63.0 PRKAG2 CCDS8777.1 1.4e-60 63.1 PRKAG1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000644 282 332 PF00571 Cystathionine beta-synthase IPR000644 358 403 PF00571 Cystathionine beta-synthase IPR000644 423 478 PF00571 Cystathionine beta-synthase HMMSmart IPR000644 202 251 SM00116 Cystathionine beta-synthase IPR000644 283 332 SM00116 Cystathionine beta-synthase IPR000644 358 406 SM00116 Cystathionine beta-synthase IPR000644 430 478 SM00116 Cystathionine beta-synthase ProfileScan IPR000644 197 258 PS51371 Cystathionine beta-synthase IPR000644 280 340 PS51371 Cystathionine beta-synthase IPR000644 355 415 PS51371 Cystathionine beta-synthase IPR000644 427 486 PS51371 Cystathionine beta-synthase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_059127 489 604976 99.8 100.0 XP_016859832 489 604976 99.8 100.0 NP_001291456 444 194200,261740,600858,602743 51.5 88.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5658 Download

>KIEE5658  1467 bp
ATGGAGCCCGGGCTGGAGCACGCACTGCGCAGGACCCCTTCCTGGAGCAGCCTTGGGGGT
TCTGAGCATCAAGAGATGAGCTTCCTAGAGCAAGAAAACAGCAGCTCATGGCCATCACCA
GCTGTGACCAGCAGCTCAGAAAGAATCCGTGGGAAACGGAGGGCCAAAGCCTTGAGATGG
ACAAGGCAGAAGTCGGTGGAGGAAGGGGAGCCACCAGGTCAGGGGGAAGGTCCCCGGTCC
AGGCCAGCTGCTGAGTCCACCGGGCTGGAGGCCACATTCCCCAAGACCACACCCTTGGCT
CAAGCTGATCCTGCCGGGGTGGGCACTCCACCAACAGGGTGGGACTGCCTCCCCTCTGAC
TGTACAGCCTCAGCTGCAGGCTCCAGCACAGATGATGTGGAGCTGGCCACGGAGTTCCCA
GCCACAGAGGCCTGGGAGTGTGAGCTAGAAGGCCTGCTGGAAGAGAGGCCTGCCCTGTGC
CTGTCCCCGCAGGCCCCATTTCCCAAGCTGGGCTGGGATGACGAACTGCGGAAACCCGGC
GCCCAGATCTACATGCGCTTCATGCAGGAGCACACCTGCTACGATGCCATGGCAACTAGC
TCCAAGCTAGTCATCTTCGACACCATGCTGGAGATCAAGAAGGCCTTCTTTGCTCTGGTG
GCCAACAGTGTGCGGGCAGCCCCTCTATGGGACAGCAAGAAGCAGAGCTTTGTGGGGATG
CTGACCATCACTGACTTCATCCTGGTGCTGCATCGCTACTACAGGTCCCCCCTGGTCCAG
ATCTATGAGATTGAACAACATAAGATTGAGACCTGGAGGGAGATCTACCTGCAAGGCTGC
TTCAAGCCTCTGGTCTCCATCTCTCCTAATGATAGCCTGTTTGAAGCTGTCTACACCCTC
ATCAAGAACCGGATCCATCGCCTGCCTGTTCTTGACCCGGTGTCAGGCAACGTACTCCAC
ATCCTCACACACAAACGCCTGCTCAAGTTCCTGCACATCTTTGGTTCCCTGCTGCCCCGG
CCCTCCTTCCTCTACCGCACTATCCAAGATTTGGGCATCGGCACATTCCGAGACTTGGCT
GTGGTGCTGGAGACAGCACCCATCCTGACTGCACTGGACATCTTTGTGGACCGGCGTGTG
TCTGCACTGCCTGTGGTCAACGAATGTGGTCAGGTCGTGGGCCTCTATTCCCGCTTTGAT
GTGATTCACCTGGCTGCCCAGCAAACCTACAACCACCTGGACATGAGTGTGGGAGAAGCC
CTGAGGCAGAGGACACTATGTCTGGAGGGAGTCCTTTCCTGCCAGCCCCACGAGAGCTTG
GGGGAAGTGATCGACAGGATTGCTCGGGAGCAGGTACACAGGCTGGTGCTAGTGGACGAG
ACCCAGCATCTCTTGGGCGTGGTCTCCCTCTCCGACATCCTTCAGGCACTGGTGCTCAGC
CCTGCTGGCATCGATGCCCTCGGGGCC

ORF Protein

Cloned ORF protein sequence for pFN21AE5658 Download

>KIEE5658  489 aa
MEPGLEHALRRTPSWSSLGGSEHQEMSFLEQENSSSWPSPAVTSSSERIRGKRRAKALRW
TRQKSVEEGEPPGQGEGPRSRPAAESTGLEATFPKTTPLAQADPAGVGTPPTGWDCLPSD
CTASAAGSSTDDVELATEFPATEAWECELEGLLEERPALCLSPQAPFPKLGWDDELRKPG
AQIYMRFMQEHTCYDAMATSSKLVIFDTMLEIKKAFFALVANSVRAAPLWDSKKQSFVGM
LTITDFILVLHRYYRSPLVQIYEIEQHKIETWREIYLQGCFKPLVSISPNDSLFEAVYTL
IKNRIHRLPVLDPVSGNVLHILTHKRLLKFLHIFGSLLPRPSFLYRTIQDLGIGTFRDLA
VVLETAPILTALDIFVDRRVSALPVVNECGQVVGLYSRFDVIHLAAQQTYNHLDMSVGEA
LRQRTLCLEGVLSCQPHESLGEVIDRIAREQVHRLVLVDETQHLLGVVSLSDILQALVLS
PAGIDALGA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5658 Download

>pFN21AE5658  6172 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGCCCGGGCTGGAGCACGCACTGCGCAGGAC
CCCTTCCTGGAGCAGCCTTGGGGGTTCTGAGCATCAAGAGATGAGCTTCCTAGAGCAAGA
AAACAGCAGCTCATGGCCATCACCAGCTGTGACCAGCAGCTCAGAAAGAATCCGTGGGAA
ACGGAGGGCCAAAGCCTTGAGATGGACAAGGCAGAAGTCGGTGGAGGAAGGGGAGCCACC
AGGTCAGGGGGAAGGTCCCCGGTCCAGGCCAGCTGCTGAGTCCACCGGGCTGGAGGCCAC
ATTCCCCAAGACCACACCCTTGGCTCAAGCTGATCCTGCCGGGGTGGGCACTCCACCAAC
AGGGTGGGACTGCCTCCCCTCTGACTGTACAGCCTCAGCTGCAGGCTCCAGCACAGATGA
TGTGGAGCTGGCCACGGAGTTCCCAGCCACAGAGGCCTGGGAGTGTGAGCTAGAAGGCCT
GCTGGAAGAGAGGCCTGCCCTGTGCCTGTCCCCGCAGGCCCCATTTCCCAAGCTGGGCTG
GGATGACGAACTGCGGAAACCCGGCGCCCAGATCTACATGCGCTTCATGCAGGAGCACAC
CTGCTACGATGCCATGGCAACTAGCTCCAAGCTAGTCATCTTCGACACCATGCTGGAGAT
CAAGAAGGCCTTCTTTGCTCTGGTGGCCAACAGTGTGCGGGCAGCCCCTCTATGGGACAG
CAAGAAGCAGAGCTTTGTGGGGATGCTGACCATCACTGACTTCATCCTGGTGCTGCATCG
CTACTACAGGTCCCCCCTGGTCCAGATCTATGAGATTGAACAACATAAGATTGAGACCTG
GAGGGAGATCTACCTGCAAGGCTGCTTCAAGCCTCTGGTCTCCATCTCTCCTAATGATAG
CCTGTTTGAAGCTGTCTACACCCTCATCAAGAACCGGATCCATCGCCTGCCTGTTCTTGA
CCCGGTGTCAGGCAACGTACTCCACATCCTCACACACAAACGCCTGCTCAAGTTCCTGCA
CATCTTTGGTTCCCTGCTGCCCCGGCCCTCCTTCCTCTACCGCACTATCCAAGATTTGGG
CATCGGCACATTCCGAGACTTGGCTGTGGTGCTGGAGACAGCACCCATCCTGACTGCACT
GGACATCTTTGTGGACCGGCGTGTGTCTGCACTGCCTGTGGTCAACGAATGTGGTCAGGT
CGTGGGCCTCTATTCCCGCTTTGATGTGATTCACCTGGCTGCCCAGCAAACCTACAACCA
CCTGGACATGAGTGTGGGAGAAGCCCTGAGGCAGAGGACACTATGTCTGGAGGGAGTCCT
TTCCTGCCAGCCCCACGAGAGCTTGGGGGAAGTGATCGACAGGATTGCTCGGGAGCAGGT
ACACAGGCTGGTGCTAGTGGACGAGACCCAGCATCTCTTGGGCGTGGTCTCCCTCTCCGA
CATCCTTCAGGCACTGGTGCTCAGCCCTGCTGGCATCGATGCCCTCGGGGCCGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT