(Linear) (Six-base) MAPSORT of: KIEE5658.seq Check: 2974 from: 1 to: 1467 KIEE5658.orf With 182 enzymes: SgfI * December 5, 2014 13:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 583 1467 Size: 583 884 AccI GT'mk_AC Cuts at: 0 890 1467 Size: 890 577 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1405 1467 Size: 1405 62 AhdI GACnn_n'nnGTC Cuts at: 0 939 1277 1467 Size: 939 338 190 Fragments arranged by size: 939 338 190 AlwNI CAG_nnn'CTG Cuts at: 0 250 1428 1467 Size: 250 1178 39 Fragments arranged by size: 1178 250 39 AvaI C'yCGr_G Cuts at: 0 7 1343 1459 1467 Size: 7 1336 116 8 Fragments arranged by size: 1336 116 8 7 BamHI G'GATC_C Cuts at: 0 911 1467 Size: 911 556 BanI G'GyrC_C Cuts at: 0 538 1467 Size: 538 929 BanII G_rGCy'C Cuts at: 0 8 1467 Size: 8 1459 BbeI G_GCGC'C Cuts at: 0 542 1467 Size: 542 925 BbvCI CC'TCA_GC Cuts at: 0 369 1467 Size: 369 1098 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1321 1355 1467 Size: 1321 34 112 Fragments arranged by size: 1321 112 34 BglI GCCn_nnn'nGGC Cuts at: 0 427 1445 1462 1467 Size: 427 1018 17 5 Fragments arranged by size: 1018 427 17 5 BglII A'GATC_T Cuts at: 0 545 779 821 1467 Size: 545 234 42 646 Fragments arranged by size: 646 545 234 42 BlpI GC'TnA_GC Cuts at: 0 1435 1467 Size: 1435 32 Bme1580I G_kGCm'C Cuts at: 0 325 1467 Size: 325 1142 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 63 286 366 649 834 1108 1303 Size: 34 29 223 80 283 185 274 195 Cuts at: 1303 1467 Size: 164 Fragments arranged by size: 283 274 223 195 185 164 80 34 29 Bpu10I CC'TnA_GC Cuts at: 0 369 1467 Size: 369 1098 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 192 284 967 1467 Size: 192 92 683 500 Fragments arranged by size: 683 500 192 92 BsaI GGTCTCn'nnnn_ Cuts at: 0 802 858 1372 1407 1467 Size: 802 56 514 35 60 Fragments arranged by size: 802 514 60 56 35 BsaHI Gr'CG_yC Cuts at: 0 539 1467 Size: 539 928 BsaWI w'CCGG_w Cuts at: 0 908 1467 Size: 908 559 BseYI C'CCAG_C Cuts at: 0 417 507 512 1217 1382 1467 Size: 417 90 5 705 165 85 Fragments arranged by size: 705 417 165 90 85 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 976 1094 1127 1467 Size: 976 118 33 340 Fragments arranged by size: 976 340 118 33 BsiHKAI G_wGCw'C Cuts at: 0 20 572 1436 1467 Size: 20 552 864 31 Fragments arranged by size: 864 552 31 20 Bsp1286I G_dGCh'C Cuts at: 0 8 20 325 572 1436 1467 Size: 8 12 305 247 864 31 Fragments arranged by size: 864 305 247 31 12 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 583 836 1341 1467 Size: 583 253 505 126 Fragments arranged by size: 583 505 253 126 BsrFI r'CCGG_y Cuts at: 0 1130 1467 Size: 1130 337 BsrGI T'GTAC_A Cuts at: 0 361 1467 Size: 361 1106 BssSI C'ACGA_G Cuts at: 0 1309 1467 Size: 1309 158 BstAPI GCAn_nnn'nTGC Cuts at: 0 24 1467 Size: 24 1443 BstXI CCAn_nnnn'nTGG Cuts at: 0 264 294 338 1467 Size: 264 30 44 1129 Fragments arranged by size: 1129 264 44 30 BstYI r'GATC_y Cuts at: 0 545 779 821 911 1467 Size: 545 234 42 90 556 Fragments arranged by size: 556 545 234 90 42 Bsu36I CC'TnA_GG Cuts at: 0 1261 1467 Size: 1261 206 BtgI C'CryG_G Cuts at: 0 147 407 587 1467 Size: 147 260 180 880 Fragments arranged by size: 880 260 180 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 736 901 1467 Size: 736 165 566 Fragments arranged by size: 736 566 165 BtsI GCAGTG_nn' Cuts at: 0 22 1144 1467 Size: 22 1122 323 Fragments arranged by size: 1122 323 22 ClaI AT'CG_AT Cuts at: 0 1451 1467 Size: 1451 16 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1146 1181 1467 Size: 1146 35 286 Fragments arranged by size: 1146 286 35 EaeI y'GGCC_r Cuts at: 0 109 404 659 1467 Size: 109 295 255 808 Fragments arranged by size: 808 295 255 109 EarI CTCTTCn'nnn_ Cuts at: 0 455 1467 Size: 455 1012 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 63 286 366 649 834 1108 1303 Size: 34 29 223 80 283 185 274 195 Cuts at: 1303 1405 1467 Size: 102 62 Fragments arranged by size: 283 274 223 195 185 102 80 62 34 29 EcoO109I rG'GnC_Cy Cuts at: 0 32 229 492 764 1467 Size: 32 197 263 272 703 Fragments arranged by size: 703 272 263 197 32 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 660 663 723 1467 Size: 660 3 60 744 Fragments arranged by size: 744 660 60 3 FspI TGC'GCA Cuts at: 0 28 1467 Size: 28 1439 HaeII r_GCGC'y Cuts at: 0 542 1467 Size: 542 925 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 925 957 1263 1295 1371 1403 1467 Size: 925 32 306 32 76 32 64 Fragments arranged by size: 925 306 76 64 32 32 32 HincII GTy'rAC Cuts at: 0 1158 1467 Size: 1158 309 KasI G'GCGC_C Cuts at: 0 538 1467 Size: 538 929 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1436 1467 Size: 1436 31 MscI TGG'CCA Cuts at: 0 111 406 661 1467 Size: 111 295 255 806 Fragments arranged by size: 806 295 255 111 MslI CAynn'nnrTG Cuts at: 0 742 1123 1246 1467 Size: 742 381 123 221 Fragments arranged by size: 742 381 221 123 MspA1I CmG'CkG Cuts at: 0 121 247 373 1467 Size: 121 126 126 1094 Fragments arranged by size: 1094 126 126 121 NarI GG'CG_CC Cuts at: 0 539 1467 Size: 539 928 NcoI C'CATG_G Cuts at: 0 587 1467 Size: 587 880 NspI r_CATG'y Cuts at: 0 555 1467 Size: 555 912 PflMI CCAn_nnn'nTGG Cuts at: 0 1050 1467 Size: 1050 417 PfoI T'CCnGG_A Cuts at: 0 40 1467 Size: 40 1427 PpuMI rG'GwC_Cy Cuts at: 0 32 229 764 1467 Size: 32 197 535 703 Fragments arranged by size: 703 535 197 32 PstI C_TGCA'G Cuts at: 0 378 1467 Size: 378 1089 PvuII CAG'CTG Cuts at: 0 121 247 373 1467 Size: 121 126 126 1094 Fragments arranged by size: 1094 126 126 121 SexAI A'CCwGG_T Cuts at: 0 213 1467 Size: 213 1254 SfcI C'TryA_G Cuts at: 0 374 759 1467 Size: 374 385 708 Fragments arranged by size: 708 385 374 SfoI GGC'GCC Cuts at: 0 540 1467 Size: 540 927 SmaI CCC'GGG Cuts at: 0 9 1467 Size: 9 1458 SmlI C'TyrA_G Cuts at: 0 171 299 982 1467 Size: 171 128 683 485 Fragments arranged by size: 683 485 171 128 SrfI GCCC'GGGC Cuts at: 0 9 1467 Size: 9 1458 StuI AGG'CCT Cuts at: 0 430 452 468 643 1467 Size: 430 22 16 175 824 Fragments arranged by size: 824 430 175 22 16 StyI C'CwwG_G Cuts at: 0 51 293 587 1467 Size: 51 242 294 880 Fragments arranged by size: 880 294 242 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1113 1467 Size: 1113 354 TatI w'GTAC_w Cuts at: 0 361 1467 Size: 361 1106 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 335 1467 Size: 335 1132 XmaI C'CCGG_G Cuts at: 0 7 1467 Size: 7 1460 XmnI GAAnn'nnTTC Cuts at: 0 643 1467 Size: 643 824 Enzymes that do cut: AarI AccI AcuI AhdI AlwNI AvaI BamHI BanI BanII BbeI BbvCI BcgI BglI BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI EaeI EarI Eco57MI EcoO109I FalI FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NcoI NspI PflMI PfoI PpuMI PstI PvuII SexAI SfcI SfoI SmaI SmlI SrfI StuI StyI TaqII TatI XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI BciVI BclI BfrBI BmgBI BmrI BmtI BplI BsaAI BsaBI BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BssHII BstBI BstEII BstZ17I DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TstI Tth111I XbaI XhoI ZraI