Kazusa ORFeome Project
In the post-genomic era, interest is shifting from comprehensive
identification of protein-coding genes to functional and structural
analyses of the proteins encoded on the genome.
Although we have identified and sequenced more than 2000 cDNAs for human
genes, each of which was designated as “KIAA” plus a four-digit
number, many of them remain to be experimentally annotated
1.
Expression-ready cDNA clones derived from genes, where the open
reading frame (ORF*) of the gene of interest is placed under the
control of an appropriate promoter, are critical tools for functional
characterization of the gene products. Moreover, in some cases, it is
necessary to produce affinity tag-fusion proteins to manipulate
structurally and chemically diverse proteins in a general approach
suitable for large-scale proteomic analysis. Therefore, we started to
prepare a set of defined protein-expression clones for as many human
genes as possible including KIAA genes by a method called
“ORFeome cloning”.
The number of expression-ready clones will increase and be
an invaluable resource for the advancement of molecular biology and
medical science.
*Although ORF is different from the protein-coding sequence (CDS) by
definition, we hereafter use ORF rather than CDS for protein-coding
sequence to avoid unnecessary confusion.
Other Human cDNA/ORF clone resources
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HUGE (Human Unidentified Gene-Encoded large
proteins
Containing KIAA cDNA clones. Please visit HUGE.
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OC (ORFeome Collaboration) clones
As a member of OC,
we started distributing the human ORF clones
(OC clones)
from the ORFeome collection.
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Kazusa Mouse cDNA project
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Rouge (ROdent Unidentified Gene-Encoded large
proteins)
Mouse homologues of KIAA and FLJ cDNA clones. Please visit Rouge.
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Collection of Flexi Clones
