################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspI AvrII BbrPI BbsI BciVI BclI BfrI BlnI BpuAI BsaAI BsaBI BseAI BseRI BsiEI BsiWI BsmBI BsmI BspEI BspHI BspLU11I BsrBI BsrDI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HgaI HindIII HpaI KpnI KspI MamI MfeI MluI MroI MseI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tsp509I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 889 GTCTAC GT^[AC][GT]AC AcyI 538 GGCGCC G[GA]^CG[CT]C AocI 1260 CCTGAGG CC^T[ACGT]AGG Asp700I 639 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 26 TGCGCA TGC^GCA BamHI 911 GGATCC G^GATCC BanI 538 GGCGCC G^G[CT][GA]CC BanII 4 GAGCCC G[GA]GC[CT]^C BbeI 538 GGCGCC GGCGC^C BbvCI 368 CCTCAGC CCTCAGC(-5/-2) BcgI 1332 CGACAGGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 1434 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1434 GCTCAGC GC^T[ACGT]AGC BsaHI 538 GGCGCC G[GA]^CG[CT]C BsaWI 908 ACCGGA [AT]^CCGG[AT] Bsp1407I 361 TGTACA T^GTACA BspDI 1450 ATCGAT AT^CGAT BsrFI 1130 ACCGGC [GA]^CCGG[CT] BsrGI 361 TGTACA T^GTACA BssSI 1309 CACGAG CACGAG(-5/-1) BstAPI 18 GCACGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1260 CCTGAGG CC^T[ACGT]AGG CelII 1434 GCTCAGC GC^T[ACGT]AGC Cfr10I 1130 ACCGGC [GA]^CCGG[CT] ClaI 1450 ATCGAT AT^CGAT EarI 460 GAAGAG CTCTTC(1/4) Eco81I 1260 CCTGAGG CC^T[ACGT]AGG FspI 26 TGCGCA TGC^GCA HaeII 538 GGCGCC [GA]GCGC^[CT] Hin1I 538 GGCGCC G[GA]^CG[CT]C HincII 1156 GTCAAC GT[CT]^[GA]AC HindII 1156 GTCAAC GT[CT]^[GA]AC KasI 538 GGCGCC G^GCGCC Ksp632I 460 GAAGAG CTCTTC(1/4) MaeII 950 ACGT A^CGT MaeIII 124 GTGAC ^GT[ACGT]AC NarI 538 GGCGCC GG^CGCC NcoI 587 CCATGG C^CATGG NspI 551 ACATGC [GA]CATG^[CT] PflMI 1044 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 374 CTGCAG CTGCA^G SexAI 213 ACCAGGT A^CC[AT]GGT SfoI 538 GGCGCC GGC^GCC SmaI 7 CCCGGG CCC^GGG SspBI 361 TGTACA T^GTACA Tsp45I 124 GTGAC ^GT[GC]AC Van91I 1044 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 328 CCACCAACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 7 CCCGGG C^CCGGG XmaI 7 CCCGGG C^CCGGG XmnI 639 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AhdI 934 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1272 GACACTATGTC AlwNI 245 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1423 CAGGCACTG AspEI 934 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1272 GACACTATGTC BsrI 1113 ACTGG ACTGG(1/-1) 1428 ACTGG Eam1105I 934 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1272 GACACTATGTC HphI 115 TCACC GGTGA(8/7) 1206 TCACC MboII 460 GAAGA GAAGA(8/7) 614 TCTTC PleI 253 GAGTC GAGTC(4/5) 1289 GAGTC SfcI 374 CTGCAG C^T[GA][CT]AG 759 CTACAG TfiI 143 GAATC G^A[AT]TC 1203 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaI AvaII BalI BbvI BcnI BfaI BglI BglII BmyI BpmI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeI MboI MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StuI StyI TaqI TseI TspRI TthHB8I XhoII