(Circular) (Six-base) MAPSORT of: pFN21AE5658.seq Check: 6660 from: 1 to: 6172 pFN21AE5658 6172 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2588 2588 Size: 6172 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3982 278 Size: 53 83 186 936 2446 2468 Fragments arranged by size: 2468 2446 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2895 3515 1937 Size: 958 620 4594 Fragments arranged by size: 4594 958 620 Acc65I G'GTAC_C Cuts at: 3493 3493 Size: 6172 AclI AA'CG_TT Cuts at: 1563 4299 4672 1563 Size: 2736 373 3063 Fragments arranged by size: 3063 2736 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3410 4235 5619 5887 1632 Size: 1778 825 1384 268 1917 Fragments arranged by size: 1917 1778 1384 825 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5975 AflIII A'CryG_T Cuts at: 1203 5071 6048 1203 Size: 3868 977 1327 Fragments arranged by size: 3868 1327 977 AgeI A'CCGG_T Cuts at: 5003 5003 Size: 6172 AhdI GACnn_n'nnGTC Cuts at: 2944 3282 4901 2944 Size: 338 1619 4215 Fragments arranged by size: 4215 1619 338 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6172 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6172 AlwNI CAG_nnn'CTG Cuts at: 2255 3433 3915 5487 2255 Size: 1178 482 1572 2940 Fragments arranged by size: 2940 1572 1178 482 ApaLI G'TGCA_C Cuts at: 4229 5385 4229 Size: 1156 5016 ApoI r'AATT_y Cuts at: 1493 3481 3707 3859 5968 1493 Size: 1988 226 152 2109 1697 Fragments arranged by size: 2109 1988 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4726 160 Size: 872 3694 1606 Fragments arranged by size: 3694 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 AvaI C'yCGr_G Cuts at: 1870 1943 2012 3348 3464 3497 1870 Size: 73 69 1336 116 33 4545 Fragments arranged by size: 4545 1336 116 73 69 33 BamHI G'GATC_C Cuts at: 2916 3502 3869 2916 Size: 586 367 5219 Fragments arranged by size: 5219 586 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2543 3493 4948 618 Size: 325 217 627 756 950 1455 1842 Fragments arranged by size: 1842 1455 950 756 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 3491 1391 Size: 622 1478 4072 Fragments arranged by size: 4072 1478 622 BbeI G_GCGC'C Cuts at: 2547 2547 Size: 6172 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5201 BbvCI CC'TCA_GC Cuts at: 2374 2374 Size: 6172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3326 3360 4363 4397 1903 Size: 34 1389 34 1003 34 3678 Fragments arranged by size: 3678 1389 1003 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4064 5274 4064 Size: 1210 4962 BclI T'GATC_A Cuts at: 1548 1548 Size: 6172 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2432 3450 3467 Size: 107 122 71 631 605 760 1018 17 Cuts at: 3467 4783 136 Size: 1316 1525 Fragments arranged by size: 1525 1316 1018 760 631 605 122 107 71 17 BglII A'GATC_T Cuts at: 2550 2784 2826 6167 2550 Size: 234 42 3341 2555 Fragments arranged by size: 3341 2555 234 42 BlpI GC'TnA_GC Cuts at: 3440 3589 3440 Size: 149 6023 Bme1580I G_kGCm'C Cuts at: 1790 2330 4233 5389 1790 Size: 540 1903 1156 2573 Fragments arranged by size: 2573 1903 1156 540 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6172 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3903 4861 442 Size: 416 53 83 399 24 2486 958 1753 Fragments arranged by size: 2486 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6172 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2039 2068 2291 2371 2654 2839 3113 3308 Size: 800 29 223 80 283 185 274 195 Cuts at: 3308 4832 1239 Size: 1524 2579 Fragments arranged by size: 2579 1524 800 283 274 223 195 185 80 29 Bpu10I CC'TnA_GC Cuts at: 2374 2374 Size: 6172 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2197 2289 2972 4297 5162 5460 2197 Size: 92 683 1325 865 298 2909 Fragments arranged by size: 2909 1325 865 683 298 92 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2807 2863 3377 3412 4835 882 Size: 617 1308 56 514 35 1423 2219 Fragments arranged by size: 2219 1423 1308 617 514 56 35 BsaAI yAC'GTr Cuts at: 493 1204 3963 493 Size: 711 2759 2702 Fragments arranged by size: 2759 2702 711 BsaBI GATnn'nnATC Cuts at: 6166 6166 Size: 6172 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2544 3979 4361 4996 Size: 53 83 186 936 1011 1435 382 635 Cuts at: 4996 275 Size: 1451 Fragments arranged by size: 1451 1435 1011 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2913 4605 5003 5277 5424 5835 2913 Size: 1692 398 274 147 411 3250 Fragments arranged by size: 3250 1692 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5947 Fragments arranged by size: 5947 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6172 BseYI C'CCAG_C Cuts at: 2422 2512 2517 3222 3387 5375 5871 2422 Size: 90 5 705 165 1988 496 2723 Fragments arranged by size: 2723 1988 705 496 165 90 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2981 3099 3132 1163 Size: 573 1245 118 33 4203 Fragments arranged by size: 4203 1245 573 118 33 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3622 4383 5411 1540 Size: 275 186 1621 761 1028 2301 Fragments arranged by size: 2301 1621 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2025 2577 3441 4233 4318 5389 2025 Size: 552 864 792 85 1071 2808 Fragments arranged by size: 2808 1071 864 792 552 85 BsmI GAATG_Cn' Cuts at: 3683 3776 3683 Size: 93 6079 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2025 2330 2577 3441 3491 4233 Size: 399 223 12 305 247 864 50 742 Cuts at: 4233 4318 5389 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 864 742 399 305 247 223 85 50 12 BspEI T'CCGG_A Cuts at: 5835 5835 Size: 6172 BspHI T'CATG_A Cuts at: 4061 5791 4061 Size: 1730 4442 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2588 2841 3346 3527 844 Size: 1744 253 505 181 3489 Fragments arranged by size: 3489 1744 505 253 181 BsrBI CCG'CTC Cuts at: 1394 1823 4059 1394 Size: 429 2236 3507 Fragments arranged by size: 3507 2236 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4667 4841 65 Size: 1183 3419 174 1396 Fragments arranged by size: 3419 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3135 4816 5003 1700 Size: 110 1325 1681 187 2869 Fragments arranged by size: 2869 1681 1325 187 110 BsrGI T'GTAC_A Cuts at: 96 2366 96 Size: 2270 3902 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6172 BssSI C'ACGA_G Cuts at: 3314 4232 5244 3314 Size: 918 1012 4242 Fragments arranged by size: 4242 1012 918 BstAPI GCAn_nnn'nTGC Cuts at: 794 2029 794 Size: 1235 4937 BstBI TT'CG_AA Cuts at: 4976 4976 Size: 6172 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6172 BstXI CCAn_nnnn'nTGG Cuts at: 2269 2299 2343 2269 Size: 30 44 6098 Fragments arranged by size: 6098 44 30 BstYI r'GATC_y Cuts at: 1972 2550 2784 2826 2916 3502 3869 4254 4271 Size: 578 234 42 90 586 367 385 17 Cuts at: 4271 5723 5809 5821 6167 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 586 578 385 367 346 234 90 86 42 17 12 Bsu36I CC'TnA_GG Cuts at: 3266 3266 Size: 6172 BtgI C'CryG_G Cuts at: 513 1065 2004 2152 2412 2592 513 Size: 552 939 148 260 180 4093 Fragments arranged by size: 4093 939 552 260 180 148 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2741 2906 487 Size: 683 56 1515 165 3753 Fragments arranged by size: 3753 1515 683 165 56 BtsI GCAGTG_nn' Cuts at: 2027 3149 3688 4482 4502 2027 Size: 1122 539 794 20 3697 Fragments arranged by size: 3697 1122 794 539 20 ClaI AT'CG_AT Cuts at: 1554 3456 1554 Size: 1902 4270 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3151 3186 529 Size: 35 2587 35 3515 Fragments arranged by size: 3515 2587 35 35 DraI TTT'AAA Cuts at: 3476 3476 Size: 6172 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5179 809 Size: 507 3863 1802 Fragments arranged by size: 3863 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2114 2409 2664 Size: 54 1425 50 275 18 284 295 255 Cuts at: 2664 3619 4508 6137 8 Size: 955 889 1629 43 Fragments arranged by size: 1629 1425 955 889 295 284 275 255 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3619 1537 Size: 275 1807 4090 Fragments arranged by size: 4090 1807 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2460 4102 1353 Size: 1107 1642 3423 Fragments arranged by size: 3423 1642 1107 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4759 5133 5279 387 Size: 1064 138 3170 374 146 1280 Fragments arranged by size: 3170 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2068 2291 2371 2654 2839 3113 Size: 393 407 29 223 80 283 185 274 Cuts at: 3113 3308 3410 4235 4832 5619 5887 1239 Size: 195 102 825 597 787 268 1524 Fragments arranged by size: 1524 825 787 597 407 393 283 274 268 223 195 185 102 80 29 EcoO109I rG'GnC_Cy Cuts at: 2037 2234 2497 2769 2037 Size: 197 263 272 5440 Fragments arranged by size: 5440 272 263 197 EcoRI G'AATT_C Cuts at: 3481 3859 3481 Size: 378 5794 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2665 2668 2728 2665 Size: 3 60 6109 Fragments arranged by size: 6109 60 3 FspI TGC'GCA Cuts at: 2033 4678 2033 Size: 2645 3527 HaeII r_GCGC'y Cuts at: 1678 2547 5319 1678 Size: 869 2772 2531 Fragments arranged by size: 2772 2531 869 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2930 2962 3268 3300 3376 Size: 32 135 32 1358 32 306 32 76 Cuts at: 3376 3408 4813 4845 4887 4919 1373 Size: 32 1405 32 42 32 2626 Fragments arranged by size: 2626 1405 1358 306 135 76 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3163 3516 3762 669 Size: 988 281 1225 353 246 3079 Fragments arranged by size: 3079 1225 988 353 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6172 HpaI GTT'AAC Cuts at: 3762 3762 Size: 6172 KasI G'GCGC_C Cuts at: 2543 2543 Size: 6172 KpnI G_GTAC'C Cuts at: 3497 3497 Size: 6172 MfeI C'AATT_G Cuts at: 3771 3771 Size: 6172 MluI A'CGCG_T Cuts at: 6048 6048 Size: 6172 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3441 5286 5470 1305 Size: 2136 1845 184 2007 Fragments arranged by size: 2136 2007 1845 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2116 2411 2666 10 Size: 54 1425 343 284 295 255 3516 Fragments arranged by size: 3516 1425 343 295 284 255 54 MslI CAynn'nnrTG Cuts at: 518 1166 2747 3128 3251 4130 4489 4648 518 Size: 648 1581 381 123 879 359 159 2042 Fragments arranged by size: 2042 1581 879 648 381 359 159 123 MspA1I CmG'CkG Cuts at: 1606 2126 2252 2378 3588 4265 5413 5658 1606 Size: 520 126 126 1210 677 1148 245 2120 Fragments arranged by size: 2120 1210 1148 677 520 245 126 126 NarI GG'CG_CC Cuts at: 2544 2544 Size: 6172 NcoI C'CATG_G Cuts at: 513 1065 2004 2592 513 Size: 552 939 588 4093 Fragments arranged by size: 4093 939 588 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6172 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6172 NotI GC'GGCC_GC Cuts at: 3619 3619 Size: 6172 NspI r_CATG'y Cuts at: 1132 1229 2560 3530 5075 1132 Size: 97 1331 970 1545 2229 Fragments arranged by size: 2229 1545 1331 970 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6172 PciI A'CATG_T Cuts at: 5071 5071 Size: 6172 PflMI CCAn_nnn'nTGG Cuts at: 1108 3055 1108 Size: 1947 4225 PfoI T'CCnGG_A Cuts at: 2045 2045 Size: 6172 PmeI GTTT'AAAC Cuts at: 3476 3476 Size: 6172 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4241 4273 5754 5786 4241 Size: 32 1481 32 4627 Fragments arranged by size: 4627 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2037 2234 2769 2037 Size: 197 535 5440 Fragments arranged by size: 5440 535 197 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6172 PsiI TTA'TAA Cuts at: 3742 3742 Size: 6172 PstI C_TGCA'G Cuts at: 830 2383 3524 830 Size: 1553 1141 3478 Fragments arranged by size: 3478 1553 1141 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6172 PvuII CAG'CTG Cuts at: 2126 2252 2378 2126 Size: 126 126 5920 Fragments arranged by size: 5920 126 126 SalI G'TCGA_C Cuts at: 1936 3514 1936 Size: 1578 4594 SbfI CC_TGCA'GG Cuts at: 3524 3524 Size: 6172 ScaI AGT'ACT Cuts at: 4420 6132 4420 Size: 1712 4460 SexAI A'CCwGG_T Cuts at: 2218 2218 Size: 6172 SfcI C'TryA_G Cuts at: 826 1045 2379 2764 3520 4655 5336 5527 826 Size: 219 1334 385 756 1135 681 191 1471 Fragments arranged by size: 1471 1334 1135 756 681 385 219 191 SfoI GGC'GCC Cuts at: 2545 2545 Size: 6172 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6172 SmaI CCC'GGG Cuts at: 1872 2014 3499 1872 Size: 142 1485 4545 Fragments arranged by size: 4545 1485 142 SmlI C'TyrA_G Cuts at: 820 1017 1943 2176 2304 2987 4276 5177 5439 Size: 197 926 233 128 683 1289 901 262 Cuts at: 5439 820 Size: 1553 Fragments arranged by size: 1553 1289 926 901 683 262 233 197 128 SnaBI TAC'GTA Cuts at: 493 3963 493 Size: 3470 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6172 SphI G_CATG'C Cuts at: 1132 3530 1132 Size: 2398 3774 SrfI GCCC'GGGC Cuts at: 2014 2014 Size: 6172 SspI AAT'ATT Cuts at: 5 52 4096 5 Size: 47 4044 2081 Fragments arranged by size: 4044 2081 47 StuI AGG'CCT Cuts at: 1517 2435 2457 2473 2648 1517 Size: 918 22 16 175 5041 Fragments arranged by size: 5041 918 175 22 16 StyI C'CwwG_G Cuts at: 513 1065 2004 2056 2298 2592 3611 513 Size: 552 939 52 242 294 1019 3074 Fragments arranged by size: 3074 1019 939 552 294 242 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3118 4199 4216 4369 4554 4999 325 Size: 1255 1538 1081 17 153 185 445 1498 Fragments arranged by size: 1538 1498 1255 1081 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2366 4418 6130 Size: 274 80 33 51 1443 389 2052 1712 Cuts at: 6130 96 Size: 138 Fragments arranged by size: 2052 1712 1443 389 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6132 6164 6132 Size: 32 6140 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6172 XbaI T'CTAG_A Cuts at: 3508 3508 Size: 6172 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2340 2340 Size: 6172 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6172 XmaI C'CCGG_G Cuts at: 1870 2012 3497 1870 Size: 142 1485 4545 Fragments arranged by size: 4545 1485 142 XmnI GAAnn'nnTTC Cuts at: 2648 4301 2648 Size: 1653 4519 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3980 276 Size: 53 83 186 936 2446 2468 Fragments arranged by size: 2468 2446 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SwaI