LGI1 ORF clone pFN21AE4520

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE4520

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Clone info.

Species

human

Clone Name

pFN21AE4520 Sequence (with vector) Restriction Map

Product ID

FHC09103

ORF Sequence

DNA sequence (1671 bp)
Protein sequence (557 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4520

Symbol

LGI1

Alias : ADLTE, ADPAEF, ADPEAF, EPITEMPIN, EPT, ETL1, IB1099

Description

leucine-rich, glioma inactivated 1, transcript variant 1

Original Clone ID

oc94520

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1671 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 557 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7431.1 5.8e-207 100.0 LGI1 CCDS81490.1 6.3e-154 91.4 LGI1 CCDS3431.1 2.9e-115 55.9 LGI2 CCDS6025.1 5.2e-112 52.0 LGI3 CCDS76325.1 2.1e-101 100.0 LGI1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001611 116 138 PF00560 Leucine-rich repeat IPR001611 140 162 PF00560 Leucine-rich repeat IPR005492 224 267 PF03736 Leucine-rich glioma-inactivated IPR005492 270 313 PF03736 Leucine-rich glioma-inactivated IPR005492 316 364 PF03736 Leucine-rich glioma-inactivated IPR005492 365 414 PF03736 Leucine-rich glioma-inactivated IPR005492 418 461 PF03736 Leucine-rich glioma-inactivated IPR005492 464 506 PF03736 Leucine-rich glioma-inactivated IPR005492 510 549 PF03736 Leucine-rich glioma-inactivated HMMSmart IPR003591 90 113 SM00369 Leucine-rich repeat IPR003591 114 137 SM00369 Leucine-rich repeat IPR003591 138 161 SM00369 Leucine-rich repeat IPR000483 173 222 SM00082 Cysteine-rich flanking region ProfileScan IPR009039 224 267 PS50912 EAR IPR009039 270 313 PS50912 EAR IPR009039 316 364 PS50912 EAR IPR009039 365 415 PS50912 EAR IPR009039 418 462 PS50912 EAR IPR009039 463 506 PS50912 EAR IPR009039 509 552 PS50912 EAR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 19 IAYFLCLLSALLLTEG 34 PRIMARY 16
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005088 557 600512,604619 100.0 100.0 NP_001295205 509 600512,604619 91.4 100.0 NP_060646 545 608301 55.9 96.6 NP_644807 548 608302 52.0 96.1 NP_644813 537 608303 48.6 96.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4520 Download

>KIEE4520  1671 bp
ATGGAATCAGAAAGAAGCAAAAGGATGGGAAATGCCTGCATTCCCCTGAAAAGAATTGCT
TATTTCCTATGTCTCTTATCTGCGCTTTTGCTGACTGAGGGGAAGAAACCAGCGAAGCCA
AAATGCCCTGCCGTGTGTACTTGTACCAAAGATAATGCTTTATGTGAGAATGCCAGATCC
ATTCCACGCACCGTTCCTCCTGATGTTATCTCATTATCCTTTGTGAGATCTGGTTTTACT
GAAATCTCAGAAGGGAGTTTTTTATTCACGCCATCGCTGCAGCTCTTGTTATTCACATCG
AACTCCTTTGATGTGATCAGTGATGATGCTTTTATTGGTCTTCCACATCTAGAGTATTTA
TTCATAGAAAACAACAACATCAAGTCAATTTCAAGACATACTTTCCGGGGACTAAAGTCA
TTAATTCACTTGAGCCTTGCAAACAACAATCTCCAGACACTCCCAAAAGATATTTTCAAA
GGCCTGGATTCTTTAACAAATGTGGACCTGAGGGGTAATTCATTTAATTGTGACTGTAAA
CTGAAATGGCTAGTGGAATGGCTTGGCCACACCAATGCAACTGTTGAAGACATCTACTGC
GAAGGCCCCCCAGAATACAAGAAGCGCAAAATCAATAGTCTCTCCTCGAAGGATTTCGAT
TGCATCATTACAGAATTTGCAAAGTCTCAAGACCTGCCTTATCAATCATTGTCCATAGAC
ACTTTTTCTTATTTGAATGATGAGTATGTAGTCATCGCTCAGCCTTTTACTGGAAAATGC
ATTTTCCTTGAATGGGACCATGTGGAAAAGACCTTCCGGAATTATGACAACATTACAGGC
ACATCCACTGTAGTATGCAAGCCTATAGTCATTGAAACTCAGCTCTATGTTATTGTGGCC
CAGCTGTTTGGTGGCTCTCACATCTATAAGCGAGACAGTTTTGCAAATAAATTCATAAAA
ATCCAGGATATTGAAATTCTCAAAATCCGAAAACCCAATGACATTGAAACATTCAAGATT
GAAAACAACTGGTACTTTGTTGTTGCTGACAGTTCAAAAGCTGGTTTTACTACCATTTAC
AAATGGAACGGAAACGGATTCTACTCCCATCAATCCTTACACGCGTGGTACAGGGACACT
GATGTGGAATATCTAGAAATAGTCAGAACACCTCAGACACTCAGAACGCCTCATTTAATT
CTGTCTAGTAGTTCCCAGCGTCCTGTAATTTATCAGTGGAACAAAGCAACACAATTATTC
ACTAACCAAACTGACATTCCTAACATGGAGGATGTGTACGCAGTGAAGCACTTCTCAGTG
AAAGGGGACGTGTACATTTGCTTGACAAGATTCATTGGTGATTCCAAAGTCATGAAATGG
GGAGGCTCCTCGTTCCAGGATATTCAGAGGATGCCATCGCGAGGATCCATGGTGTTCCAG
CCTCTTCAAATAAATAATTACCAATATGCAATTCTTGGAAGTGATTACTCCTTTACTCAA
GTGTATAACTGGGATGCAGAGAAAGCCAAATTTGTGAAATTTCAGGAATTAAATGTTCAG
GCACCAAGATCATTCACACATGTGTCCATTAATAAGCGTAATTTTCTTTTTGCTTCCAGT
TTTAAGGGAAATACACAGATTTACAAACATGTCATAGTTGACTTAAGCGCA

ORF Protein

Cloned ORF protein sequence for pFN21AE4520 Download

>KIEE4520  557 aa
MESERSKRMGNACIPLKRIAYFLCLLSALLLTEGKKPAKPKCPAVCTCTKDNALCENARS
IPRTVPPDVISLSFVRSGFTEISEGSFLFTPSLQLLLFTSNSFDVISDDAFIGLPHLEYL
FIENNNIKSISRHTFRGLKSLIHLSLANNNLQTLPKDIFKGLDSLTNVDLRGNSFNCDCK
LKWLVEWLGHTNATVEDIYCEGPPEYKKRKINSLSSKDFDCIITEFAKSQDLPYQSLSID
TFSYLNDEYVVIAQPFTGKCIFLEWDHVEKTFRNYDNITGTSTVVCKPIVIETQLYVIVA
QLFGGSHIYKRDSFANKFIKIQDIEILKIRKPNDIETFKIENNWYFVVADSSKAGFTTIY
KWNGNGFYSHQSLHAWYRDTDVEYLEIVRTPQTLRTPHLILSSSSQRPVIYQWNKATQLF
TNQTDIPNMEDVYAVKHFSVKGDVYICLTRFIGDSKVMKWGGSSFQDIQRMPSRGSMVFQ
PLQINNYQYAILGSDYSFTQVYNWDAEKAKFVKFQELNVQAPRSFTHVSINKRNFLFASS
FKGNTQIYKHVIVDLSA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4520 Download

>pFN21AE4520  6376 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAATCAGAAAGAAGCAAAAGGATGGGAAATGC
CTGCATTCCCCTGAAAAGAATTGCTTATTTCCTATGTCTCTTATCTGCGCTTTTGCTGAC
TGAGGGGAAGAAACCAGCGAAGCCAAAATGCCCTGCCGTGTGTACTTGTACCAAAGATAA
TGCTTTATGTGAGAATGCCAGATCCATTCCACGCACCGTTCCTCCTGATGTTATCTCATT
ATCCTTTGTGAGATCTGGTTTTACTGAAATCTCAGAAGGGAGTTTTTTATTCACGCCATC
GCTGCAGCTCTTGTTATTCACATCGAACTCCTTTGATGTGATCAGTGATGATGCTTTTAT
TGGTCTTCCACATCTAGAGTATTTATTCATAGAAAACAACAACATCAAGTCAATTTCAAG
ACATACTTTCCGGGGACTAAAGTCATTAATTCACTTGAGCCTTGCAAACAACAATCTCCA
GACACTCCCAAAAGATATTTTCAAAGGCCTGGATTCTTTAACAAATGTGGACCTGAGGGG
TAATTCATTTAATTGTGACTGTAAACTGAAATGGCTAGTGGAATGGCTTGGCCACACCAA
TGCAACTGTTGAAGACATCTACTGCGAAGGCCCCCCAGAATACAAGAAGCGCAAAATCAA
TAGTCTCTCCTCGAAGGATTTCGATTGCATCATTACAGAATTTGCAAAGTCTCAAGACCT
GCCTTATCAATCATTGTCCATAGACACTTTTTCTTATTTGAATGATGAGTATGTAGTCAT
CGCTCAGCCTTTTACTGGAAAATGCATTTTCCTTGAATGGGACCATGTGGAAAAGACCTT
CCGGAATTATGACAACATTACAGGCACATCCACTGTAGTATGCAAGCCTATAGTCATTGA
AACTCAGCTCTATGTTATTGTGGCCCAGCTGTTTGGTGGCTCTCACATCTATAAGCGAGA
CAGTTTTGCAAATAAATTCATAAAAATCCAGGATATTGAAATTCTCAAAATCCGAAAACC
CAATGACATTGAAACATTCAAGATTGAAAACAACTGGTACTTTGTTGTTGCTGACAGTTC
AAAAGCTGGTTTTACTACCATTTACAAATGGAACGGAAACGGATTCTACTCCCATCAATC
CTTACACGCGTGGTACAGGGACACTGATGTGGAATATCTAGAAATAGTCAGAACACCTCA
GACACTCAGAACGCCTCATTTAATTCTGTCTAGTAGTTCCCAGCGTCCTGTAATTTATCA
GTGGAACAAAGCAACACAATTATTCACTAACCAAACTGACATTCCTAACATGGAGGATGT
GTACGCAGTGAAGCACTTCTCAGTGAAAGGGGACGTGTACATTTGCTTGACAAGATTCAT
TGGTGATTCCAAAGTCATGAAATGGGGAGGCTCCTCGTTCCAGGATATTCAGAGGATGCC
ATCGCGAGGATCCATGGTGTTCCAGCCTCTTCAAATAAATAATTACCAATATGCAATTCT
TGGAAGTGATTACTCCTTTACTCAAGTGTATAACTGGGATGCAGAGAAAGCCAAATTTGT
GAAATTTCAGGAATTAAATGTTCAGGCACCAAGATCATTCACACATGTGTCCATTAATAA
GCGTAATTTTCTTTTTGCTTCCAGTTTTAAGGGAAATACACAGATTTACAAACATGTCAT
AGTTGACTTAAGCGCAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT