################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccI AciI AclI AcyI AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BanII BbeI BbrPI BbvCI BcgI BciVI BglI BlnI BmyI BsaAI BsaBI BsaHI BsaI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I BspDI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FauI FseI FspI HaeII Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MunI NaeI NarI NdeI NgoMIV NheI NotI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccIII 815 TCCGGA T^CCGGA AflII 1662 CTTAAG C^TTAAG AocI 507 CCTGAGG CC^T[ACGT]AGG BalI 564 TGGCCA TGG^CCA BamHI 1423 GGATCC G^GATCC BanI 1560 GGCACC G^G[CT][GA]CC BclI 314 TGATCA T^GATCA BcnI 405 CCGGG CC^[GC]GG BfrI 1662 CTTAAG C^TTAAG BglII 226 AGATCT A^GATCT BlpI 757 GCTCAGC GC^T[ACGT]AGC BpmI 451 CTCCAG CTGGAG(16/14) Bpu1102I 757 GCTCAGC GC^T[ACGT]AGC BsaWI 815 TCCGGA [AT]^CCGG[AT] BseAI 815 TCCGGA T^CCGGA Bsp1407I 1331 TGTACA T^GTACA BspEI 815 TCCGGA T^CCGGA BspHI 1370 TCATGA T^CATGA BspMI 692 ACCTGC ACCTGC(4/8) BsrGI 1331 TGTACA T^GTACA BstXI 900 CCAGCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 507 CCTGAGG CC^T[ACGT]AGG CelII 757 GCTCAGC GC^T[ACGT]AGC DraII 603 AGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 1427 CCATGG C^C[GA][CT]GG EaeI 564 TGGCCA [CT]^GGCC[GA] EarI 1442 CTCTTC CTCTTC(1/4) Eco81I 507 CCTGAGG CC^T[ACGT]AGG EcoO109I 603 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoT14I 1427 CCATGG C^C[AT][AT]GG EcoT22I 777 ATGCAT ATGCA^T FbaI 314 TGATCA T^GATCA HgaI 1218 GCGTC GACGC(5/10) HincII 1657 GTTGAC GT[CT]^[GA]AC HindII 1657 GTTGAC GT[CT]^[GA]AC HphI 1357 GGTGA GGTGA(8/7) Ksp632I 1442 CTCTTC CTCTTC(1/4) MaeII 1328 ACGT A^CGT MaeIII 530 GTGAC ^GT[ACGT]AC MluI 1121 ACGCGT A^CGCGT MluNI 564 TGGCCA TGG^CCA MroI 815 TCCGGA T^CCGGA MscI 564 TGGCCA TGG^CCA MspA1I 901 CAGCTG C[AC]G^C[GT]G NciI 405 CCGGG CC^[GC]GG NcoI 1427 CCATGG C^CATGG NruI 1417 TCGCGA TCG^CGA NsiI 777 ATGCAT ATGCA^T PshAI 410 GACTAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 277 CTGCAG CTGCA^G PvuII 901 CAGCTG CAG^CTG RcaI 1370 TCATGA T^CATGA SspBI 1331 TGTACA T^GTACA StuI 480 AGGCCT AGG^CCT StyI 1427 CCATGG C^C[AT][AT]GG Tsp45I 530 GTGAC ^GT[GC]AC XcmI 900 CCAGCTGTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 1122 CGCG CG^CG 1418 CGCG AseI 420 ATTAAT AT^TAAT 1588 ATTAAT AsnI 420 ATTAAT AT^TAAT 1588 ATTAAT Asp700I 648 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1305 GAAGCACTTC AvaII 504 GGACC G^G[AT]CC 795 GGACC BbsI 586 GAAGAC GAAGAC(2/6) 338 GTCTTC BbvI 279 GCAGC GCAGC(8/12) 276 GCTGC BpuAI 586 GAAGAC GAAGAC(2/6) 338 GTCTTC BsaJI 405 CCGGGG C^C[ACGT][ACGT]GG 1427 CCATGG BseRI 642 CTCCTC GAGGAG(10/8) 1386 CTCCTC BsmI 168 GAATGC GAATGC(1/-1) 38 GCATTC BspLU11I 1578 ACATGT A^CATGT 1647 ACATGT BstUI 1122 CGCG CG^CG 1418 CGCG Cac8I 34 GCCTGC GC[ACGT]^[ACGT]GC 857 GCAAGC Fnu4HI 276 GCTGC GC^[ACGT]GC 279 GCAGC HapII 405 CCGG C^CGG 816 CCGG HpaII 405 CCGG C^CGG 816 CCGG ItaI 276 GCTGC GC^[ACGT]GC 279 GCAGC MspI 405 CCGG C^CGG 816 CCGG MvnI 1122 CGCG CG^CG 1418 CGCG MwoI 270 GCCATCGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1516 GCAGAGAAAGC NspI 1578 ACATGT [GA]CATG^[CT] 1647 ACATGT PshBI 420 ATTAAT AT^TAAT 1588 ATTAAT TseI 276 GCTGC G^C[AT]GC 279 GCAGC XbaI 348 TCTAGA T^CTAGA 1152 TCTAGA XmnI 648 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1305 GAAGCACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AcsI AfaI AflIII AluI AlwI ApoI BfaI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI BstYI CfoI Cfr13I DdeI DpnII EcoRII FokI HaeIII HhaI HinP1I HinfI MaeI MboI MboII MflI MnlI MseI MslI MvaI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I Tsp509I TspRI TthHB8I XhoII