(Linear) (Six-base) MAPSORT of: KIEE4520.seq Check: 8096 from: 1 to: 1671 KIEE4520.orf With 182 enzymes: SgfI * March 22, 2012 18:15 .. AflII C'TTAA_G Cuts at: 0 1662 1671 Size: 1662 9 AflIII A'CryG_T Cuts at: 0 1121 1328 1578 1647 1671 Size: 1121 207 250 69 24 Fragments arranged by size: 1121 250 207 69 24 AleI CACnn'nnGTG Cuts at: 0 1141 1579 1671 Size: 1141 438 92 Fragments arranged by size: 1141 438 92 ApoI r'AATT_y Cuts at: 0 673 949 974 1528 1537 1671 Size: 673 276 25 554 9 134 Fragments arranged by size: 673 554 276 134 25 9 AseI AT'TA_AT Cuts at: 0 421 1589 1671 Size: 421 1168 82 Fragments arranged by size: 1168 421 82 BamHI G'GATC_C Cuts at: 0 1423 1671 Size: 1423 248 BanI G'GyrC_C Cuts at: 0 1560 1671 Size: 1560 111 BbsI GAAGACnn'nnnn_ Cuts at: 0 331 593 1671 Size: 331 262 1078 Fragments arranged by size: 1078 331 262 BclI T'GATC_A Cuts at: 0 314 1671 Size: 314 1357 BfrBI ATG'CAT Cuts at: 0 779 1671 Size: 779 892 BglII A'GATC_T Cuts at: 0 226 1671 Size: 226 1445 BlpI GC'TnA_GC Cuts at: 0 758 1671 Size: 758 913 BmgBI CAC'GTC Cuts at: 0 1329 1671 Size: 1329 342 BmrI ACTGGGnnnn_n' Cuts at: 0 1518 1671 Size: 1518 153 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 436 1671 Size: 436 1235 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 450 671 1481 1671 Size: 450 221 810 190 Fragments arranged by size: 810 450 221 190 BsaWI w'CCGG_w Cuts at: 0 815 1671 Size: 815 856 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 180 210 1279 1309 1671 Size: 180 30 1069 30 362 Fragments arranged by size: 1069 362 180 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 633 1377 1671 Size: 633 744 294 Fragments arranged by size: 744 633 294 BseYI C'CCAG_C Cuts at: 0 899 1214 1671 Size: 899 315 457 Fragments arranged by size: 899 457 315 BsmI GAATG_Cn' Cuts at: 0 38 174 1671 Size: 38 136 1497 Fragments arranged by size: 1497 136 38 BspEI T'CCGG_A Cuts at: 0 815 1671 Size: 815 856 BspHI T'CATG_A Cuts at: 0 1370 1671 Size: 1370 301 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 701 1671 Size: 701 970 BsrGI T'GTAC_A Cuts at: 0 1331 1671 Size: 1331 340 BstXI CCAn_nnnn'nTGG Cuts at: 0 907 1671 Size: 907 764 BstYI r'GATC_y Cuts at: 0 175 226 1423 1671 Size: 175 51 1197 248 Fragments arranged by size: 1197 248 175 51 Bsu36I CC'TnA_GG Cuts at: 0 508 1671 Size: 508 1163 BtgI C'CryG_G Cuts at: 0 1427 1671 Size: 1427 244 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 257 738 1400 1671 Size: 257 481 662 271 Fragments arranged by size: 662 481 271 257 BtsI GCAGTG_nn' Cuts at: 0 1307 1671 Size: 1307 364 EaeI y'GGCC_r Cuts at: 0 564 1671 Size: 564 1107 EarI CTCTTCn'nnn_ Cuts at: 0 1448 1671 Size: 1448 223 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 436 1671 Size: 436 1235 EcoO109I rG'GnC_Cy Cuts at: 0 604 1671 Size: 604 1067 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 74 416 1671 Size: 74 342 1255 Fragments arranged by size: 1255 342 74 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 193 225 1671 Size: 193 32 1446 Fragments arranged by size: 1446 193 32 HincII GTy'rAC Cuts at: 0 1659 1671 Size: 1659 12 MluI A'CGCG_T Cuts at: 0 1121 1671 Size: 1121 550 MscI TGG'CCA Cuts at: 0 566 1671 Size: 566 1105 MslI CAynn'nnrTG Cuts at: 0 572 1141 1579 1671 Size: 572 569 438 92 Fragments arranged by size: 572 569 438 92 MspA1I CmG'CkG Cuts at: 0 903 1671 Size: 903 768 NcoI C'CATG_G Cuts at: 0 1427 1671 Size: 1427 244 NruI TCG'CGA Cuts at: 0 1419 1671 Size: 1419 252 NsiI A_TGCA'T Cuts at: 0 781 1671 Size: 781 890 NspI r_CATG'y Cuts at: 0 1582 1651 1671 Size: 1582 69 20 Fragments arranged by size: 1582 69 20 PciI A'CATG_T Cuts at: 0 1578 1647 1671 Size: 1578 69 24 Fragments arranged by size: 1578 69 24 PfoI T'CCnGG_A Cuts at: 0 962 1394 1671 Size: 962 432 277 Fragments arranged by size: 962 432 277 PshAI GACnn'nnGTC Cuts at: 0 414 1671 Size: 414 1257 PstI C_TGCA'G Cuts at: 0 281 1671 Size: 281 1390 PvuII CAG'CTG Cuts at: 0 903 1671 Size: 903 768 SfcI C'TryA_G Cuts at: 0 277 848 863 1671 Size: 277 571 15 808 Fragments arranged by size: 808 571 277 15 SmlI C'TyrA_G Cuts at: 0 429 686 1496 1662 1671 Size: 429 257 810 166 9 Fragments arranged by size: 810 429 257 166 9 StuI AGG'CCT Cuts at: 0 482 1671 Size: 482 1189 StyI C'CwwG_G Cuts at: 0 1427 1671 Size: 1427 244 TatI w'GTAC_w Cuts at: 0 136 1331 1671 Size: 136 1195 340 Fragments arranged by size: 1195 340 136 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 180 212 1415 1447 1671 Size: 180 32 1203 32 224 Fragments arranged by size: 1203 224 180 32 32 XbaI T'CTAG_A Cuts at: 0 348 1152 1671 Size: 348 804 519 Fragments arranged by size: 804 519 348 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 907 1671 Size: 907 764 XmnI GAAnn'nnTTC Cuts at: 0 652 1309 1671 Size: 652 657 362 Fragments arranged by size: 657 652 362 Enzymes that do cut: AflII AflIII AleI ApoI AseI BamHI BanI BbsI BclI BfrBI BglII BlpI BmgBI BmrI BpmI BpuEI BsaWI BsaXI BseRI BseYI BsmI BspEI BspHI BspMI BsrGI BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI Eco57MI EcoO109I FalI Hin4I HincII MluI MscI MslI MspA1I NcoI NruI NsiI NspI PciI PfoI PshAI PstI PvuII SfcI SmlI StuI StyI TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BanII BbeI BbvCI BcgI BciVI BglI Bme1580I BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MmeI NaeI NarI NdeI NgoMIV NheI NotI PacI PasI PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XhoI XmaI ZraI