(Circular) (Six-base) MAPSORT of: pFN21AE4520.seq Check: 4639 from: 1 to: 6376 pFN21AE4520 6376 bp With 182 enzymes: SgfI * March 28, 2012 14:08 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4186 278 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3719 1937 Size: 1782 4594 Acc65I G'GTAC_C Cuts at: 3697 3697 Size: 6376 AclI AA'CG_TT Cuts at: 1563 4503 4876 1563 Size: 2940 373 3063 Fragments arranged by size: 3063 2940 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4439 5823 6091 1632 Size: 2807 1384 268 1917 Fragments arranged by size: 2807 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 3667 820 Size: 197 2650 3529 Fragments arranged by size: 3529 2650 197 AflIII A'CryG_T Cuts at: 1203 3126 3333 3583 3652 5275 6252 1203 Size: 1923 207 250 69 1623 977 1327 Fragments arranged by size: 1923 1623 1327 977 250 207 69 AgeI A'CCGG_T Cuts at: 5207 5207 Size: 6376 AhdI GACnn_n'nnGTC Cuts at: 5105 5105 Size: 6376 AleI CACnn'nnGTG Cuts at: 1166 3146 3584 1166 Size: 1980 438 3958 Fragments arranged by size: 3958 1980 438 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6376 AlwNI CAG_nnn'CTG Cuts at: 4119 5691 4119 Size: 1572 4804 ApaLI G'TGCA_C Cuts at: 4433 5589 4433 Size: 1156 5220 ApoI r'AATT_y Cuts at: 1493 2678 2954 2979 3533 3542 3685 3911 4063 Size: 1185 276 25 554 9 143 226 152 Cuts at: 4063 6172 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1185 554 276 226 152 143 25 9 AseI AT'TA_AT Cuts at: 160 1032 2426 3594 4930 160 Size: 872 1394 1168 1336 1606 Fragments arranged by size: 1606 1394 1336 1168 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 AvaI C'yCGr_G Cuts at: 1870 1943 3701 1870 Size: 73 1758 4545 Fragments arranged by size: 4545 1758 73 BamHI G'GATC_C Cuts at: 3428 3706 4073 3428 Size: 278 367 5731 Fragments arranged by size: 5731 367 278 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3565 3697 5152 618 Size: 325 217 627 1778 132 1455 1842 Fragments arranged by size: 1842 1778 1455 627 325 217 132 BanII G_rGCy'C Cuts at: 1391 3695 1391 Size: 2304 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2336 2598 928 Size: 971 437 262 4706 Fragments arranged by size: 4706 971 437 262 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4567 4601 1903 Size: 34 2630 34 3678 Fragments arranged by size: 3678 2630 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4268 5478 4268 Size: 1210 5166 BclI T'GATC_A Cuts at: 1548 2319 1548 Size: 771 5605 BfrBI ATG'CAT Cuts at: 2784 2784 Size: 6376 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4987 136 Size: 107 122 71 631 605 3315 1525 Fragments arranged by size: 3315 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2231 6371 2231 Size: 4140 2236 BlpI GC'TnA_GC Cuts at: 2763 3793 2763 Size: 1030 5346 Bme1580I G_kGCm'C Cuts at: 1790 4437 5593 1790 Size: 2647 1156 2573 Fragments arranged by size: 2647 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3334 1318 Size: 2016 4360 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3523 4107 5065 Size: 416 53 83 399 24 2106 584 958 Cuts at: 5065 442 Size: 1753 Fragments arranged by size: 2106 1753 958 584 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6376 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2441 5036 1239 Size: 1202 2595 2579 Fragments arranged by size: 2595 2579 1202 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2455 2676 3486 4501 5366 5664 2455 Size: 221 810 1015 865 298 3167 Fragments arranged by size: 3167 1015 865 810 298 221 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5039 882 Size: 617 3540 2219 Fragments arranged by size: 3540 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4167 493 Size: 711 2963 2702 Fragments arranged by size: 2963 2702 711 BsaBI GATnn'nnATC Cuts at: 6370 6370 Size: 6376 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4183 4565 5200 275 Size: 53 83 186 936 2650 382 635 1451 Fragments arranged by size: 2650 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2820 4809 5207 5481 5628 6039 2820 Size: 1989 398 274 147 411 3157 Fragments arranged by size: 3157 1989 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2185 2215 3284 3314 1178 Size: 30 165 30 782 30 1069 30 4240 Fragments arranged by size: 4240 1069 782 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2638 3382 1186 Size: 1452 744 4180 Fragments arranged by size: 4180 1452 744 BseYI C'CCAG_C Cuts at: 2904 3219 5579 6075 2904 Size: 315 2360 496 3205 Fragments arranged by size: 3205 2360 496 315 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5803 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3826 4587 5615 1540 Size: 275 186 1825 761 1028 2301 Fragments arranged by size: 2301 1825 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4437 4522 5593 4437 Size: 85 1071 5220 Fragments arranged by size: 5220 1071 85 BsmI GAATG_Cn' Cuts at: 2043 2179 3887 3980 2043 Size: 136 1708 93 4439 Fragments arranged by size: 4439 1708 136 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3695 4437 4522 5593 1391 Size: 399 1905 742 85 1071 2174 Fragments arranged by size: 2174 1905 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2820 6039 2820 Size: 3219 3157 BspHI T'CATG_A Cuts at: 3375 4265 5995 3375 Size: 890 1730 3756 Fragments arranged by size: 3756 1730 890 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2706 3731 844 Size: 1862 1025 3489 Fragments arranged by size: 3489 1862 1025 BsrBI CCG'CTC Cuts at: 1394 1823 4263 1394 Size: 429 2440 3507 Fragments arranged by size: 3507 2440 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4871 5045 65 Size: 1183 3623 174 1396 Fragments arranged by size: 3623 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5020 5207 1700 Size: 110 3210 187 2869 Fragments arranged by size: 3210 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3336 96 Size: 3240 3136 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6376 BssSI C'ACGA_G Cuts at: 4436 5448 4436 Size: 1012 5364 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6376 BstBI TT'CG_AA Cuts at: 5180 5180 Size: 6376 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6376 BstXI CCAn_nnnn'nTGG Cuts at: 2912 2912 Size: 6376 BstYI r'GATC_y Cuts at: 1972 2180 2231 3428 3706 4073 4458 4475 5927 Size: 208 51 1197 278 367 385 17 1452 Cuts at: 5927 6013 6025 6371 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1197 385 367 346 278 208 86 51 17 12 Bsu36I CC'TnA_GG Cuts at: 2513 2513 Size: 6376 BtgI C'CryG_G Cuts at: 513 1065 2004 3432 513 Size: 552 939 1428 3457 Fragments arranged by size: 3457 1428 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2262 2743 3405 487 Size: 683 56 1036 481 662 3458 Fragments arranged by size: 3458 1036 683 662 481 56 BtsI GCAGTG_nn' Cuts at: 3312 3892 4686 4706 3312 Size: 580 794 20 4982 Fragments arranged by size: 4982 794 580 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6376 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6341 DraI TTT'AAA Cuts at: 3680 3680 Size: 6376 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5383 809 Size: 507 4067 1802 Fragments arranged by size: 4067 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2569 3823 4712 Size: 54 1425 50 275 18 739 1254 889 Cuts at: 4712 6341 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1254 889 739 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3823 1537 Size: 275 2011 4090 Fragments arranged by size: 4090 2011 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3453 4306 1353 Size: 2100 853 3423 Fragments arranged by size: 3423 2100 853 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4963 5337 5483 387 Size: 1064 138 3374 374 146 1280 Fragments arranged by size: 3374 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2441 4439 5036 5823 6091 1239 Size: 393 809 1998 597 787 268 1524 Fragments arranged by size: 1998 1524 809 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2609 2609 Size: 6376 EcoRI G'AATT_C Cuts at: 3685 4063 3685 Size: 378 5998 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2079 2421 2079 Size: 342 6034 FspI TGC'GCA Cuts at: 4882 4882 Size: 6376 HaeII r_GCGC'y Cuts at: 1678 5523 1678 Size: 3845 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2198 2230 5017 5049 5091 Size: 32 135 32 626 32 2787 32 42 Cuts at: 5091 5123 1373 Size: 32 2626 Fragments arranged by size: 2787 2626 626 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3664 3720 3966 669 Size: 988 281 1726 56 246 3079 Fragments arranged by size: 3079 1726 988 281 246 56 HindIII A'AGCT_T Cuts at: 748 748 Size: 6376 HpaI GTT'AAC Cuts at: 3966 3966 Size: 6376 KpnI G_GTAC'C Cuts at: 3701 3701 Size: 6376 MfeI C'AATT_G Cuts at: 3975 3975 Size: 6376 MluI A'CGCG_T Cuts at: 3126 6252 3126 Size: 3126 3250 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5490 5674 1305 Size: 4185 184 2007 Fragments arranged by size: 4185 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2571 10 Size: 54 1425 343 739 3815 Fragments arranged by size: 3815 1425 739 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2577 3146 3584 4334 4693 4852 518 Size: 648 1411 569 438 750 359 159 2042 Fragments arranged by size: 2042 1411 750 648 569 438 359 159 MspA1I CmG'CkG Cuts at: 1606 2908 3792 4469 5617 5862 1606 Size: 1302 884 677 1148 245 2120 Fragments arranged by size: 2120 1302 1148 884 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3432 513 Size: 552 939 1428 3457 Fragments arranged by size: 3457 1428 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6376 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6376 NotI GC'GGCC_GC Cuts at: 3823 3823 Size: 6376 NruI TCG'CGA Cuts at: 3424 3424 Size: 6376 NsiI A_TGCA'T Cuts at: 2786 2786 Size: 6376 NspI r_CATG'y Cuts at: 1132 1229 3587 3656 3734 5279 1132 Size: 97 2358 69 78 1545 2229 Fragments arranged by size: 2358 2229 1545 97 78 69 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6376 PciI A'CATG_T Cuts at: 3583 3652 5275 3583 Size: 69 1623 4684 Fragments arranged by size: 4684 1623 69 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6376 PfoI T'CCnGG_A Cuts at: 2967 3399 2967 Size: 432 5944 PmeI GTTT'AAAC Cuts at: 3680 3680 Size: 6376 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4445 4477 5958 5990 4445 Size: 32 1481 32 4831 Fragments arranged by size: 4831 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2419 1613 Size: 806 5570 PsiI TTA'TAA Cuts at: 3946 3946 Size: 6376 PstI C_TGCA'G Cuts at: 830 2286 3728 830 Size: 1456 1442 3478 Fragments arranged by size: 3478 1456 1442 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6376 PvuII CAG'CTG Cuts at: 2908 2908 Size: 6376 SalI G'TCGA_C Cuts at: 1936 3718 1936 Size: 1782 4594 SbfI CC_TGCA'GG Cuts at: 3728 3728 Size: 6376 ScaI AGT'ACT Cuts at: 4624 6336 4624 Size: 1712 4664 SfcI C'TryA_G Cuts at: 826 1045 2282 2853 2868 3724 4859 5540 5731 Size: 219 1237 571 15 856 1135 681 191 Cuts at: 5731 826 Size: 1471 Fragments arranged by size: 1471 1237 1135 856 681 571 219 191 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6376 SmaI CCC'GGG Cuts at: 1872 3703 1872 Size: 1831 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2434 2691 3501 3667 4480 5381 Size: 197 926 491 257 810 166 813 901 Cuts at: 5381 5643 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 813 810 491 262 257 197 166 SnaBI TAC'GTA Cuts at: 493 4167 493 Size: 3674 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6376 SphI G_CATG'C Cuts at: 1132 3734 1132 Size: 2602 3774 SspI AAT'ATT Cuts at: 5 52 4300 5 Size: 47 4248 2081 Fragments arranged by size: 4248 2081 47 StuI AGG'CCT Cuts at: 1517 2487 1517 Size: 970 5406 StyI C'CwwG_G Cuts at: 513 1065 2004 3432 3815 513 Size: 552 939 1428 383 3074 Fragments arranged by size: 3074 1428 939 552 383 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4403 4420 4573 4758 5203 325 Size: 1255 2823 17 153 185 445 1498 Fragments arranged by size: 2823 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2141 3336 4622 Size: 274 80 33 51 1443 164 1195 1286 Cuts at: 4622 6334 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1286 1195 274 164 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2185 2217 3420 3452 6336 6368 2185 Size: 32 1203 32 2884 32 2193 Fragments arranged by size: 2884 2193 1203 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6376 XbaI T'CTAG_A Cuts at: 2353 3157 3712 2353 Size: 804 555 5017 Fragments arranged by size: 5017 804 555 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2912 2912 Size: 6376 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6376 XmaI C'CCGG_G Cuts at: 1870 3701 1870 Size: 1831 4545 XmnI GAAnn'nnTTC Cuts at: 2657 3314 4505 2657 Size: 657 1191 4528 Fragments arranged by size: 4528 1191 657 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4184 276 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI