BRAP ORF clone FHC24886

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC24886

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Clone info.

Species

human

Clone Name

pFN21AE2014 Sequence (with vector) Restriction Map

Product ID

FHC24886

ORF Sequence

DNA sequence (1776 bp)
Protein sequence (592 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590810]

Gene

KIEE2014

Symbol

BRAP

Alias : BRAP2, IMP, RNF52

Description

BRCA1 associated protein

Original Clone ID

cs02914

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1776 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 592 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9154.1 1e-131 100.0 BRAP The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition BlastProDom IPR011422 124 243 PD017029 BRCA1-associated 2 HMMPfam IPR011422 149 257 PF07576 BRCA1-associated 2 IPR018957 264 303 PF00097 Zinc finger IPR001607 317 376 PF02148 Zinc finger HMMSmart IPR001841 264 303 SM00184 Zinc finger IPR001607 316 365 SM00290 Zinc finger ProfileScan IPR001841 264 304 PS50089 Zinc finger IPR001607 315 376 PS50271 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_006759 592 604986 100.0 100.0 XP_005254001 633 604986 93.4 98.5 XP_016875481 538 604986 90.9 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2014 Download

>KIEE2014  1776 bp
ATGAGTGTGTCACTGGTTGTTATCCGATTGGAGCTCGCGGAACACTCGCCTGTCCCCGCC
GGCTTCGGCTTCAGCGCCGCGGCCGGGGAAATGTCTGATGAGGAGATAAAAAAGACGACA
CTAGCCTCAGCTGTAGCCTGTTTAGAAGGCAAGTCACCAGGAGAGAAAGTAGCGATTATC
CATCAGCATCTCGGCCGTCGAGAAATGACAGATGTGATCATTGAGACCATGAAGTCCAAC
CCAGATGAACTAAAAACTACAGTGGAAGAAAGGAAGTCTTCAGAAGCCTCCCCCACTGCG
CAAAGAAGTAAAGATCACAGTAAGGAATGCATAAACGCTGCCCCAGATTCTCCGTCCAAA
CAGCTTCCAGACCAGATTTCATTCTTCAGTGGAAATCCATCAGTTGAAATAGTTCATGGT
ATTATGCACCTATATAAGACAAATAAGATGACCTCCTTAAAAGAAGATGTGCGGCGCAGT
GCCATGCTGTGTATTCTCACAGTCCCTGCTGCAATGACCAGTCATGACCTTATGAAGTTT
GTTGCCCCATTTAACGAAGTAATTGAACAAATGAAAATTATCAGAGACTCTACTCCCAAC
CAATATATGGTGCTGATAAAGTTTCGTGCACAGGCTGATGCGGATAGTTTTTATATGACA
TGCAATGGCCGCCAGTTCAACTCAATAGAAGATGACGTTTGCCAGCTAGTGTATGTGGAA
AGAGCTGAAGTGCTCAAATCTGAAGATGGCGCCAGCCTCCCAGTGATGGACCTGACTGAA
CTCCCCAAGTGCACGGTGTGTCTGGAGCGCATGGACGAGTCTGTGAATGGCATCCTCACA
ACGTTATGTAACCACAGCTTCCACAGCCAGTGTCTACAGCGCTGGGACGATACCACGTGT
CCTGTTTGCCGGTACTGTCAAACGCCCGAGCCAGTAGAAGAAAATAAGTGTTTTGAGTGT
GGTGTTCAGGAAAATCTTTGGATTTGTTTAATATGCGGCCACATAGGATGTGGACGGTAT
GTCAGTCGACATGCTTATAAGCACTTTGAGGAAACGCAGCACACGTATGCCATGCAGCTT
ACCAACCATCGAGTCTGGGACTATGCTGGAGATAACTATGTTCATCGACTGGTTGCAAGT
AAAACAGATGGAAAAATAGTACAGTATGAATGTGAGGGGGATACTTGCCAGGAAGAGAAA
ATAGATGCCTTACAGTTAGAGTATTCATATTTACTAACAAGCCAGCTGGAATCTCAGCGA
ATCTACTGGGAAAACAAGATAGTTCGGATAGAGAAGGACACAGCAGAGGAAATTAACAAC
ATGAAGACCAAGTTTAAAGAAACAATTGAGAAGTGTGATAATCTAGAGCACAAACTAAAT
GATCTCCTAAAAGAAAAGCAGTCTGTGGAAAGAAAGTGCACTCAGCTAAACACAAAAGTG
GCCAAACTCACCAACGAGCTCAAAGAGGAGCAGGAAATGAACAAGTGTTTGCGAGCCAAC
CAAGTCCTCCTGCAGAACAAGCTAAAAGAGGAGGAGAGGGTGCTGAAGGAGACCTGTGAC
CAAAAAGATCTGCAGATCACCGAGATCCAGGAGCAGCTGCGTGACGTCATGTTCTACCTG
GAGACACAGCAGAAGATCAACCATCTGCCTGCCGAGACCCGGCAGGAAATCCAGGAGGGA
CAGATCAACATCGCCATGGCCTCGGCCTCGAGCCCTGCCTCTTCGGGGGGCAGTGGGAAG
TTGCCCTCCAGGAAGGGCCGCAGCAAGAGGGGCAAG

ORF Protein

Cloned ORF protein sequence for pFN21AE2014 Download

>KIEE2014  592 aa
MSVSLVVIRLELAEHSPVPAGFGFSAAAGEMSDEEIKKTTLASAVACLEGKSPGEKVAII
HQHLGRREMTDVIIETMKSNPDELKTTVEERKSSEASPTAQRSKDHSKECINAAPDSPSK
QLPDQISFFSGNPSVEIVHGIMHLYKTNKMTSLKEDVRRSAMLCILTVPAAMTSHDLMKF
VAPFNEVIEQMKIIRDSTPNQYMVLIKFRAQADADSFYMTCNGRQFNSIEDDVCQLVYVE
RAEVLKSEDGASLPVMDLTELPKCTVCLERMDESVNGILTTLCNHSFHSQCLQRWDDTTC
PVCRYCQTPEPVEENKCFECGVQENLWICLICGHIGCGRYVSRHAYKHFEETQHTYAMQL
TNHRVWDYAGDNYVHRLVASKTDGKIVQYECEGDTCQEEKIDALQLEYSYLLTSQLESQR
IYWENKIVRIEKDTAEEINNMKTKFKETIEKCDNLEHKLNDLLKEKQSVERKCTQLNTKV
AKLTNELKEEQEMNKCLRANQVLLQNKLKEEERVLKETCDQKDLQITEIQEQLRDVMFYL
ETQQKINHLPAETRQEIQEGQINIAMASASSPASSGGSGKLPSRKGRSKRGK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2014 Download

>pFN21AE2014  6481 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGTGTGTCACTGGTTGTTATCCGATTGGAGCT
CGCGGAACACTCGCCTGTCCCCGCCGGCTTCGGCTTCAGCGCCGCGGCCGGGGAAATGTC
TGATGAGGAGATAAAAAAGACGACACTAGCCTCAGCTGTAGCCTGTTTAGAAGGCAAGTC
ACCAGGAGAGAAAGTAGCGATTATCCATCAGCATCTCGGCCGTCGAGAAATGACAGATGT
GATCATTGAGACCATGAAGTCCAACCCAGATGAACTAAAAACTACAGTGGAAGAAAGGAA
GTCTTCAGAAGCCTCCCCCACTGCGCAAAGAAGTAAAGATCACAGTAAGGAATGCATAAA
CGCTGCCCCAGATTCTCCGTCCAAACAGCTTCCAGACCAGATTTCATTCTTCAGTGGAAA
TCCATCAGTTGAAATAGTTCATGGTATTATGCACCTATATAAGACAAATAAGATGACCTC
CTTAAAAGAAGATGTGCGGCGCAGTGCCATGCTGTGTATTCTCACAGTCCCTGCTGCAAT
GACCAGTCATGACCTTATGAAGTTTGTTGCCCCATTTAACGAAGTAATTGAACAAATGAA
AATTATCAGAGACTCTACTCCCAACCAATATATGGTGCTGATAAAGTTTCGTGCACAGGC
TGATGCGGATAGTTTTTATATGACATGCAATGGCCGCCAGTTCAACTCAATAGAAGATGA
CGTTTGCCAGCTAGTGTATGTGGAAAGAGCTGAAGTGCTCAAATCTGAAGATGGCGCCAG
CCTCCCAGTGATGGACCTGACTGAACTCCCCAAGTGCACGGTGTGTCTGGAGCGCATGGA
CGAGTCTGTGAATGGCATCCTCACAACGTTATGTAACCACAGCTTCCACAGCCAGTGTCT
ACAGCGCTGGGACGATACCACGTGTCCTGTTTGCCGGTACTGTCAAACGCCCGAGCCAGT
AGAAGAAAATAAGTGTTTTGAGTGTGGTGTTCAGGAAAATCTTTGGATTTGTTTAATATG
CGGCCACATAGGATGTGGACGGTATGTCAGTCGACATGCTTATAAGCACTTTGAGGAAAC
GCAGCACACGTATGCCATGCAGCTTACCAACCATCGAGTCTGGGACTATGCTGGAGATAA
CTATGTTCATCGACTGGTTGCAAGTAAAACAGATGGAAAAATAGTACAGTATGAATGTGA
GGGGGATACTTGCCAGGAAGAGAAAATAGATGCCTTACAGTTAGAGTATTCATATTTACT
AACAAGCCAGCTGGAATCTCAGCGAATCTACTGGGAAAACAAGATAGTTCGGATAGAGAA
GGACACAGCAGAGGAAATTAACAACATGAAGACCAAGTTTAAAGAAACAATTGAGAAGTG
TGATAATCTAGAGCACAAACTAAATGATCTCCTAAAAGAAAAGCAGTCTGTGGAAAGAAA
GTGCACTCAGCTAAACACAAAAGTGGCCAAACTCACCAACGAGCTCAAAGAGGAGCAGGA
AATGAACAAGTGTTTGCGAGCCAACCAAGTCCTCCTGCAGAACAAGCTAAAAGAGGAGGA
GAGGGTGCTGAAGGAGACCTGTGACCAAAAAGATCTGCAGATCACCGAGATCCAGGAGCA
GCTGCGTGACGTCATGTTCTACCTGGAGACACAGCAGAAGATCAACCATCTGCCTGCCGA
GACCCGGCAGGAAATCCAGGAGGGACAGATCAACATCGCCATGGCCTCGGCCTCGAGCCC
TGCCTCTTCGGGGGGCAGTGGGAAGTTGCCCTCCAGGAAGGGCCGCAGCAAGAGGGGCAA
GGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
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