(Circular) (Six-base) MAPSORT of: pFN21AE2014.seq Check: 3899 from: 1 to: 6481 pFN21AE2014 6481 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3612 4291 278 Size: 53 83 186 936 2076 679 2468 Fragments arranged by size: 2468 2076 936 679 186 83 53 AccI GT'mk_AC Cuts at: 1937 2878 3031 3824 1937 Size: 941 153 793 4594 Fragments arranged by size: 4594 941 793 153 Acc65I G'GTAC_C Cuts at: 3802 3802 Size: 6481 AclI AA'CG_TT Cuts at: 1563 2846 4608 4981 1563 Size: 1283 1762 373 3063 Fragments arranged by size: 3063 1762 1283 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2059 2268 2374 2751 2766 3569 4544 5928 Size: 427 209 106 377 15 803 975 1384 Cuts at: 5928 6196 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 975 803 427 377 268 209 106 15 AfeI AGC'GCT Cuts at: 2885 2885 Size: 6481 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6284 AflIII A'CryG_T Cuts at: 1203 2900 3066 5380 6357 1203 Size: 1697 166 2314 977 1327 Fragments arranged by size: 2314 1697 1327 977 166 AgeI A'CCGG_T Cuts at: 5312 5312 Size: 6481 AhdI GACnn_n'nnGTC Cuts at: 3023 5210 3023 Size: 2187 4294 AleI CACnn'nnGTG Cuts at: 1166 3440 1166 Size: 2274 4207 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2504 3087 1184 Size: 1320 583 4578 Fragments arranged by size: 4578 1320 583 AlwNI CAG_nnn'CTG Cuts at: 2510 4224 5796 2510 Size: 1714 1572 3195 Fragments arranged by size: 3195 1714 1572 ApaLI G'TGCA_C Cuts at: 2631 2794 3421 4538 5694 2631 Size: 163 627 1117 1156 3418 Fragments arranged by size: 3418 1156 1117 627 163 ApoI r'AATT_y Cuts at: 1493 3790 4016 4168 6277 1493 Size: 2297 226 152 2109 1697 Fragments arranged by size: 2297 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5035 160 Size: 872 4003 1606 Fragments arranged by size: 4003 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 AvaI C'yCGr_G Cuts at: 1870 1943 2930 3712 3806 1870 Size: 73 987 782 94 4545 Fragments arranged by size: 4545 987 782 94 73 BamHI G'GATC_C Cuts at: 3811 4178 3811 Size: 367 6114 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2753 3802 5257 618 Size: 325 217 627 966 1049 1455 1842 Fragments arranged by size: 1842 1455 1049 966 627 325 217 BanII G_rGCy'C Cuts at: 1391 2040 3465 3719 3800 1391 Size: 649 1425 254 81 4072 Fragments arranged by size: 4072 1425 649 254 81 BbeI G_GCGC'C Cuts at: 2757 2757 Size: 6481 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2274 3335 928 Size: 971 375 1061 4074 Fragments arranged by size: 4074 1061 971 375 BbvCI CC'TCA_GC Cuts at: 2131 2131 Size: 6481 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4672 4706 1903 Size: 34 2735 34 3678 Fragments arranged by size: 3678 2735 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3178 4373 5583 3178 Size: 1195 1210 4076 Fragments arranged by size: 4076 1210 1195 BclI T'GATC_A Cuts at: 1548 2220 1548 Size: 672 5809 BfrBI ATG'CAT Cuts at: 2334 2334 Size: 6481 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5092 136 Size: 107 122 71 631 605 3420 1525 Fragments arranged by size: 3420 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3571 6476 3571 Size: 2905 3576 BlpI GC'TnA_GC Cuts at: 3898 3898 Size: 6481 Bme1580I G_kGCm'C Cuts at: 1790 2635 2798 3425 4542 5698 1790 Size: 845 163 627 1117 1156 2573 Fragments arranged by size: 2573 1156 1117 845 627 163 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6481 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2759 3280 4212 Size: 416 53 83 399 24 1342 521 932 Cuts at: 4212 5170 442 Size: 958 1753 Fragments arranged by size: 1753 1342 958 932 521 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6481 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2828 3132 3644 3736 5141 1239 Size: 1589 304 512 92 1405 2579 Fragments arranged by size: 2579 1589 1405 512 304 92 Bpu10I CC'TnA_GC Cuts at: 2131 2131 Size: 6481 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4606 5471 5769 4606 Size: 865 298 5318 Fragments arranged by size: 5318 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2222 3548 3653 5144 882 Size: 617 723 1326 105 1491 2219 Fragments arranged by size: 2219 1491 1326 723 617 105 BsaAI yAC'GTr Cuts at: 493 1204 2901 3069 4272 493 Size: 711 1697 168 1203 2702 Fragments arranged by size: 2702 1697 1203 711 168 BsaBI GATnn'nnATC Cuts at: 3692 6475 3692 Size: 2783 3698 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2754 3609 4288 4670 Size: 53 83 186 936 1221 855 679 382 Cuts at: 4670 5305 275 Size: 635 1451 Fragments arranged by size: 1451 1221 936 855 679 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4914 5312 5586 5733 6144 4914 Size: 398 274 147 411 5251 Fragments arranged by size: 5251 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2157 2187 1178 Size: 30 165 30 754 30 5472 Fragments arranged by size: 5472 754 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 3485 3548 3551 1186 Size: 934 1365 63 3 4116 Fragments arranged by size: 4116 1365 934 63 3 BseYI C'CCAG_C Cuts at: 2886 5684 6180 2886 Size: 2798 496 3187 Fragments arranged by size: 3187 2798 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5908 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2088 2200 3931 4692 5720 1540 Size: 275 186 87 112 1731 761 1028 2301 Fragments arranged by size: 2301 1731 1028 761 275 186 112 87 BsiHKAI G_wGCw'C Cuts at: 2040 2635 2739 2798 3375 3425 3465 4542 4627 Size: 595 104 59 577 50 40 1077 85 Cuts at: 4627 5698 2040 Size: 1071 2823 Fragments arranged by size: 2823 1077 1071 595 577 104 85 59 50 40 BsmI GAATG_Cn' Cuts at: 2336 3992 4085 2336 Size: 1656 93 4732 Fragments arranged by size: 4732 1656 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2635 2739 2798 3375 3425 3465 Size: 399 250 595 104 59 577 50 40 Cuts at: 3465 3719 3800 4542 4627 5698 1391 Size: 254 81 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 595 577 399 254 250 104 85 81 59 50 40 BspEI T'CCGG_A Cuts at: 6144 6144 Size: 6481 BspHI T'CATG_A Cuts at: 2527 4370 6100 2527 Size: 1843 1730 2908 Fragments arranged by size: 2908 1843 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3836 844 Size: 2992 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4368 1394 Size: 429 2545 3507 Fragments arranged by size: 3507 2545 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2523 2674 4976 5150 65 Size: 1183 1275 151 2302 174 1396 Fragments arranged by size: 2302 1396 1275 1183 174 151 BsrFI r'CCGG_y Cuts at: 1700 1810 2063 2913 5125 5312 1700 Size: 110 253 850 2212 187 2869 Fragments arranged by size: 2869 2212 850 253 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6481 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6481 BssSI C'ACGA_G Cuts at: 4541 5553 4541 Size: 1012 5469 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6481 BstBI TT'CG_AA Cuts at: 5285 5285 Size: 6481 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6481 BstYI r'GATC_y Cuts at: 1972 3571 3588 3811 4178 4563 4580 6032 6118 Size: 1599 17 223 367 385 17 1452 86 Cuts at: 6118 6130 6476 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1599 1452 385 367 346 223 86 17 17 12 BtgI C'CryG_G Cuts at: 513 1065 2082 3699 513 Size: 552 1017 1617 3295 Fragments arranged by size: 3295 1617 1017 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3679 487 Size: 683 56 2453 3289 Fragments arranged by size: 3289 2453 683 56 BtsI GCAGTG_nn' Cuts at: 2298 2488 3742 3997 4791 4811 2298 Size: 190 1254 255 794 20 3968 Fragments arranged by size: 3968 1254 794 255 190 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6481 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6446 DraI TTT'AAA Cuts at: 3340 3785 3340 Size: 445 6036 DraIII CAC_nnn'GTG Cuts at: 2802 2802 Size: 6481 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2236 5488 809 Size: 507 920 3252 1802 Fragments arranged by size: 3252 1802 920 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 2197 2671 Size: 54 1425 50 275 18 255 112 474 Cuts at: 2671 3001 3444 3928 4817 6446 8 Size: 330 443 484 889 1629 43 Fragments arranged by size: 1629 1425 889 484 474 443 330 275 255 112 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2085 2197 3928 1537 Size: 275 273 112 1731 4090 Fragments arranged by size: 4090 1731 275 273 112 EarI CTCTTCn'nnn_ Cuts at: 1353 3192 3730 4411 1353 Size: 1839 538 681 3423 Fragments arranged by size: 3423 1839 681 538 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5068 5442 5588 387 Size: 1064 138 3479 374 146 1280 Fragments arranged by size: 3479 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2038 3463 2038 Size: 1425 5056 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2059 2268 2374 2751 2766 2828 3132 Size: 393 427 209 106 377 15 62 304 Cuts at: 3132 3569 3644 3736 4544 5141 5928 6196 1239 Size: 437 75 92 808 597 787 268 1524 Fragments arranged by size: 1524 808 787 597 437 427 393 377 304 268 209 106 92 75 62 15 EcoRI G'AATT_C Cuts at: 3790 4168 3790 Size: 378 6103 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2298 2298 Size: 6481 FspI TGC'GCA Cuts at: 2304 4987 2304 Size: 2683 3798 HaeII r_GCGC'y Cuts at: 1678 2082 2757 2887 5628 1678 Size: 404 675 130 2741 2531 Fragments arranged by size: 2741 2531 675 404 130 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2022 2054 2170 2202 2342 Size: 32 135 32 450 32 116 32 140 Cuts at: 2342 2374 3009 3041 3631 3663 5122 5154 5196 Size: 32 635 32 590 32 1459 32 42 Cuts at: 5196 5228 1373 Size: 32 2626 Fragments arranged by size: 2626 1459 635 590 450 140 135 116 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3032 3825 4071 669 Size: 988 281 1094 793 246 3079 Fragments arranged by size: 3079 1094 988 793 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6481 HpaI GTT'AAC Cuts at: 4071 4071 Size: 6481 KasI G'GCGC_C Cuts at: 2753 2753 Size: 6481 KpnI G_GTAC'C Cuts at: 3806 3806 Size: 6481 MfeI C'AATT_G Cuts at: 3348 4080 3348 Size: 732 5749 MluI A'CGCG_T Cuts at: 6357 6357 Size: 6481 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2265 5595 5779 1305 Size: 960 3330 184 2007 Fragments arranged by size: 3330 2007 960 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3446 10 Size: 54 1425 343 1614 3045 Fragments arranged by size: 3045 1614 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3069 3440 3698 4439 4798 4957 Size: 648 843 1060 371 258 741 359 159 Cuts at: 4957 518 Size: 2042 Fragments arranged by size: 2042 1060 843 741 648 371 359 258 159 MspA1I CmG'CkG Cuts at: 1606 2084 2135 3250 3601 3897 4574 5722 5967 Size: 478 51 1115 351 296 677 1148 245 Cuts at: 5967 1606 Size: 2120 Fragments arranged by size: 2120 1148 1115 677 478 351 296 245 51 NaeI GCC'GGC Cuts at: 2065 2065 Size: 6481 NarI GG'CG_CC Cuts at: 2754 2754 Size: 6481 NcoI C'CATG_G Cuts at: 513 1065 3699 513 Size: 552 2634 3295 Fragments arranged by size: 3295 2634 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6481 NgoMIV G'CCGG_C Cuts at: 2063 2063 Size: 6481 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6481 NotI GC'GGCC_GC Cuts at: 3928 3928 Size: 6481 NsiI A_TGCA'T Cuts at: 2336 2336 Size: 6481 NspI r_CATG'y Cuts at: 1132 1229 2667 3038 3839 5384 1132 Size: 97 1438 371 801 1545 2229 Fragments arranged by size: 2229 1545 1438 801 371 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6481 PciI A'CATG_T Cuts at: 5380 5380 Size: 6481 PflMI CCAn_nnn'nTGG Cuts at: 1108 2611 1108 Size: 1503 4978 PfoI T'CCnGG_A Cuts at: 3591 3675 3752 3591 Size: 84 77 6320 Fragments arranged by size: 6320 84 77 PmeI GTTT'AAAC Cuts at: 3785 3785 Size: 6481 PmlI CAC'GTG Cuts at: 2901 2901 Size: 6481 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4550 4582 6063 6095 4550 Size: 32 1481 32 4936 Fragments arranged by size: 4936 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6481 PsiI TTA'TAA Cuts at: 3042 4051 3042 Size: 1009 5472 PspXI vC'TCGA_Gb Cuts at: 3712 3712 Size: 6481 PstI C_TGCA'G Cuts at: 830 3519 3579 3833 830 Size: 2689 60 254 3478 Fragments arranged by size: 3478 2689 254 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6481 PvuII CAG'CTG Cuts at: 2135 3250 3601 2135 Size: 1115 351 5015 Fragments arranged by size: 5015 1115 351 SacI G_AGCT'C Cuts at: 2040 3465 2040 Size: 1425 5056 SacII CC_GC'GG Cuts at: 2085 2085 Size: 6481 SalI G'TCGA_C Cuts at: 1936 3030 3823 1936 Size: 1094 793 4594 Fragments arranged by size: 4594 1094 793 SbfI CC_TGCA'GG Cuts at: 3833 3833 Size: 6481 ScaI AGT'ACT Cuts at: 4729 6441 4729 Size: 1712 4769 SfcI C'TryA_G Cuts at: 826 1045 2136 2262 2879 3515 3575 3829 4964 Size: 219 1091 126 617 636 60 254 1135 Cuts at: 4964 5645 5836 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1091 681 636 617 254 219 191 126 60 SfoI GGC'GCC Cuts at: 2755 2755 Size: 6481 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6481 SmaI CCC'GGG Cuts at: 1872 3808 1872 Size: 1936 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3712 4585 5486 5748 820 Size: 197 926 1769 873 901 262 1553 Fragments arranged by size: 1769 1553 926 901 873 262 197 SnaBI TAC'GTA Cuts at: 493 4272 493 Size: 3779 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6481 SphI G_CATG'C Cuts at: 1132 3839 1132 Size: 2707 3774 SspI AAT'ATT Cuts at: 5 52 4405 5 Size: 47 4353 2081 Fragments arranged by size: 4353 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6481 StyI C'CwwG_G Cuts at: 513 1065 3699 3920 513 Size: 552 2634 221 3074 Fragments arranged by size: 3074 2634 552 221 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4508 4525 4678 4863 5308 325 Size: 1255 2928 17 153 185 445 1498 Fragments arranged by size: 2928 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3163 4727 6439 Size: 274 80 33 51 1443 1186 1564 1712 Cuts at: 6439 96 Size: 138 Fragments arranged by size: 1712 1564 1443 1186 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3404 3436 6441 6473 3404 Size: 32 3005 32 3412 Fragments arranged by size: 3412 3005 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6481 XbaI T'CTAG_A Cuts at: 3367 3817 3367 Size: 450 6031 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2608 3011 2608 Size: 403 6078 XhoI C'TCGA_G Cuts at: 1943 3712 1943 Size: 1769 4712 XmaI C'CCGG_G Cuts at: 1870 3806 1870 Size: 1936 4545 XmnI GAAnn'nnTTC Cuts at: 2415 4610 2415 Size: 2195 4286 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3610 4289 276 Size: 53 83 186 936 2076 679 2468 Fragments arranged by size: 2468 2076 936 679 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI FspAI NruI PacI PpuMI PspOMI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI