(Linear) (Six-base) MAPSORT of: KIEE2014.seq Check: 278 from: 1 to: 1776 KIEE2014.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AatII G_ACGT'C Cuts at: 0 1607 1776 Size: 1607 169 AccI GT'mk_AC Cuts at: 0 873 1026 1776 Size: 873 153 750 Fragments arranged by size: 873 750 153 AclI AA'CG_TT Cuts at: 0 841 1776 Size: 841 935 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 54 263 369 746 761 1564 1776 Size: 54 209 106 377 15 803 212 Fragments arranged by size: 803 377 212 209 106 54 15 AfeI AGC'GCT Cuts at: 0 880 1776 Size: 880 896 AflIII A'CryG_T Cuts at: 0 895 1061 1776 Size: 895 166 715 Fragments arranged by size: 895 715 166 AhdI GACnn_n'nnGTC Cuts at: 0 1018 1776 Size: 1018 758 AleI CACnn'nnGTG Cuts at: 0 1435 1776 Size: 1435 341 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 499 1082 1776 Size: 499 583 694 Fragments arranged by size: 694 583 499 AlwNI CAG_nnn'CTG Cuts at: 0 505 1776 Size: 505 1271 ApaLI G'TGCA_C Cuts at: 0 626 789 1416 1776 Size: 626 163 627 360 Fragments arranged by size: 627 626 360 163 AvaI C'yCGr_G Cuts at: 0 925 1707 1776 Size: 925 782 69 Fragments arranged by size: 925 782 69 BanI G'GyrC_C Cuts at: 0 748 1776 Size: 748 1028 BanII G_rGCy'C Cuts at: 0 35 1460 1714 1776 Size: 35 1425 254 62 Fragments arranged by size: 1425 254 62 35 BbeI G_GCGC'C Cuts at: 0 752 1776 Size: 752 1024 BbsI GAAGACnn'nnnn_ Cuts at: 0 269 1330 1776 Size: 269 1061 446 Fragments arranged by size: 1061 446 269 BbvCI CC'TCA_GC Cuts at: 0 126 1776 Size: 126 1650 BciVI GTATCCnnnnn_n' Cuts at: 0 1173 1776 Size: 1173 603 BclI T'GATC_A Cuts at: 0 215 1776 Size: 215 1561 BfrBI ATG'CAT Cuts at: 0 329 1776 Size: 329 1447 BglII A'GATC_T Cuts at: 0 1566 1776 Size: 1566 210 Bme1580I G_kGCm'C Cuts at: 0 630 793 1420 1776 Size: 630 163 627 356 Fragments arranged by size: 630 627 356 163 BmrI ACTGGGnnnn_n' Cuts at: 0 754 1275 1776 Size: 754 521 501 Fragments arranged by size: 754 521 501 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 823 1127 1639 1731 1776 Size: 823 304 512 92 45 Fragments arranged by size: 823 512 304 92 45 Bpu10I CC'TnA_GC Cuts at: 0 126 1776 Size: 126 1650 BsaI GGTCTCn'nnnn_ Cuts at: 0 217 1543 1648 1776 Size: 217 1326 105 128 Fragments arranged by size: 1326 217 128 105 BsaAI yAC'GTr Cuts at: 0 896 1064 1776 Size: 896 168 712 Fragments arranged by size: 896 712 168 BsaBI GATnn'nnATC Cuts at: 0 1687 1776 Size: 1687 89 BsaHI Gr'CG_yC Cuts at: 0 749 1604 1776 Size: 749 855 172 Fragments arranged by size: 855 749 172 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 152 182 1776 Size: 152 30 1594 Fragments arranged by size: 1594 152 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 115 1480 1543 1546 1776 Size: 115 1365 63 3 230 Fragments arranged by size: 1365 230 115 63 3 BseYI C'CCAG_C Cuts at: 0 881 1776 Size: 881 895 BsiEI CG_ry'CG Cuts at: 0 83 195 1776 Size: 83 112 1581 Fragments arranged by size: 1581 112 83 BsiHKAI G_wGCw'C Cuts at: 0 35 630 734 793 1370 1420 1460 1776 Size: 35 595 104 59 577 50 40 316 Fragments arranged by size: 595 577 316 104 59 50 40 35 BsmI GAATG_Cn' Cuts at: 0 331 1776 Size: 331 1445 Bsp1286I G_dGCh'C Cuts at: 0 35 630 734 793 1370 1420 1460 1714 Size: 35 595 104 59 577 50 40 254 Cuts at: 1714 1776 Size: 62 Fragments arranged by size: 595 577 254 104 62 59 50 40 35 BspHI T'CATG_A Cuts at: 0 522 1776 Size: 522 1254 BsrDI GCAATG_nn' Cuts at: 0 518 669 1776 Size: 518 151 1107 Fragments arranged by size: 1107 518 151 BsrFI r'CCGG_y Cuts at: 0 58 908 1776 Size: 58 850 868 Fragments arranged by size: 868 850 58 BstYI r'GATC_y Cuts at: 0 1566 1583 1776 Size: 1566 17 193 Fragments arranged by size: 1566 193 17 BtgI C'CryG_G Cuts at: 0 77 1694 1776 Size: 77 1617 82 Fragments arranged by size: 1617 82 77 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1674 1776 Size: 1674 102 BtsI GCAGTG_nn' Cuts at: 0 293 483 1737 1776 Size: 293 190 1254 39 Fragments arranged by size: 1254 293 190 39 DraI TTT'AAA Cuts at: 0 1335 1776 Size: 1335 441 DraIII CAC_nnn'GTG Cuts at: 0 797 1776 Size: 797 979 DrdI GACnn_nn'nnGTC Cuts at: 0 231 1776 Size: 231 1545 EaeI y'GGCC_r Cuts at: 0 80 192 666 996 1439 1776 Size: 80 112 474 330 443 337 Fragments arranged by size: 474 443 337 330 112 80 EagI C'GGCC_G Cuts at: 0 80 192 1776 Size: 80 112 1584 Fragments arranged by size: 1584 112 80 EarI CTCTTCn'nnn_ Cuts at: 0 1187 1725 1776 Size: 1187 538 51 Fragments arranged by size: 1187 538 51 EcoICRI GAG'CTC Cuts at: 0 33 1458 1776 Size: 33 1425 318 Fragments arranged by size: 1425 318 33 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 54 263 369 746 761 823 1127 1564 Size: 54 209 106 377 15 62 304 437 Cuts at: 1564 1639 1731 1776 Size: 75 92 45 Fragments arranged by size: 437 377 304 209 106 92 75 62 54 45 15 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 293 1776 Size: 293 1483 FspI TGC'GCA Cuts at: 0 299 1776 Size: 299 1477 HaeII r_GCGC'y Cuts at: 0 77 752 882 1776 Size: 77 675 130 894 Fragments arranged by size: 894 675 130 77 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 17 49 165 197 337 369 1004 1036 Size: 17 32 116 32 140 32 635 32 Cuts at: 1036 1626 1658 1776 Size: 590 32 118 Fragments arranged by size: 635 590 140 118 116 32 32 32 32 32 17 HincII GTy'rAC Cuts at: 0 1027 1776 Size: 1027 749 KasI G'GCGC_C Cuts at: 0 748 1776 Size: 748 1028 MfeI C'AATT_G Cuts at: 0 1343 1776 Size: 1343 433 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 260 1776 Size: 260 1516 MscI TGG'CCA Cuts at: 0 1441 1776 Size: 1441 335 MslI CAynn'nnrTG Cuts at: 0 1064 1435 1693 1776 Size: 1064 371 258 83 Fragments arranged by size: 1064 371 258 83 MspA1I CmG'CkG Cuts at: 0 79 130 1245 1596 1776 Size: 79 51 1115 351 180 Fragments arranged by size: 1115 351 180 79 51 NaeI GCC'GGC Cuts at: 0 60 1776 Size: 60 1716 NarI GG'CG_CC Cuts at: 0 749 1776 Size: 749 1027 NcoI C'CATG_G Cuts at: 0 1694 1776 Size: 1694 82 NgoMIV G'CCGG_C Cuts at: 0 58 1776 Size: 58 1718 NsiI A_TGCA'T Cuts at: 0 331 1776 Size: 331 1445 NspI r_CATG'y Cuts at: 0 662 1033 1776 Size: 662 371 743 Fragments arranged by size: 743 662 371 PflMI CCAn_nnn'nTGG Cuts at: 0 606 1776 Size: 606 1170 PfoI T'CCnGG_A Cuts at: 0 1586 1670 1747 1776 Size: 1586 84 77 29 Fragments arranged by size: 1586 84 77 29 PmlI CAC'GTG Cuts at: 0 896 1776 Size: 896 880 PsiI TTA'TAA Cuts at: 0 1037 1776 Size: 1037 739 PspXI vC'TCGA_Gb Cuts at: 0 1707 1776 Size: 1707 69 PstI C_TGCA'G Cuts at: 0 1514 1574 1776 Size: 1514 60 202 Fragments arranged by size: 1514 202 60 PvuII CAG'CTG Cuts at: 0 130 1245 1596 1776 Size: 130 1115 351 180 Fragments arranged by size: 1115 351 180 130 SacI G_AGCT'C Cuts at: 0 35 1460 1776 Size: 35 1425 316 Fragments arranged by size: 1425 316 35 SacII CC_GC'GG Cuts at: 0 80 1776 Size: 80 1696 SalI G'TCGA_C Cuts at: 0 1025 1776 Size: 1025 751 SfcI C'TryA_G Cuts at: 0 131 257 874 1510 1570 1776 Size: 131 126 617 636 60 206 Fragments arranged by size: 636 617 206 131 126 60 SfoI GGC'GCC Cuts at: 0 750 1776 Size: 750 1026 SmlI C'TyrA_G Cuts at: 0 1707 1776 Size: 1707 69 StyI C'CwwG_G Cuts at: 0 1694 1776 Size: 1694 82 TatI w'GTAC_w Cuts at: 0 1158 1776 Size: 1158 618 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1399 1431 1776 Size: 1399 32 345 Fragments arranged by size: 1399 345 32 XbaI T'CTAG_A Cuts at: 0 1362 1776 Size: 1362 414 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 603 1006 1776 Size: 603 403 770 Fragments arranged by size: 770 603 403 XhoI C'TCGA_G Cuts at: 0 1707 1776 Size: 1707 69 XmnI GAAnn'nnTTC Cuts at: 0 410 1776 Size: 410 1366 ZraI GAC'GTC Cuts at: 0 1605 1776 Size: 1605 171 Enzymes that do cut: AatII AccI AclI AcuI AfeI AflIII AhdI AleI AlfI AlwNI ApaLI AvaI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrFI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI FalI FspI HaeII Hin4I HincII KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PflMI PfoI PmlI PsiI PspXI PstI PvuII SacI SacII SalI SfcI SfoI SmlI StyI TatI TstI XbaI XcmI XhoI XmnI ZraI Enzymes that do not cut: AarI Acc65I AflII AgeI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI BglI BlpI BmgBI BmtI BplI BpuEI BsaWI BsgI BsiWI BsmBI BspEI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI EciI EcoNI EcoO109I EcoRI EcoRV FseI FspAI HindIII HpaI KpnI MluI NdeI NheI NotI NruI PacI PasI PciI PmeI PpiI PpuMI PshAI PspOMI PsrI PvuI RsrII SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I XmaI