################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## Acc65I AccIII AcsI AflII AgeI AocI ApaI ApoI AscI AseI AsnI Asp718I AspI AvrII BamHI BcgI BfrI BglI BlnI BlpI Bpu1102I BsaWI BseAI BsgI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraII Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FseI HgaI HindIII HpaI KpnI MluI MroI NdeI NheI NotI NruI NspV PacI PflFI PinAI PmeI PpuMI PshAI PshBI PvuI RsrII SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AatII 1603 GACGTC GACGT^C AclI 840 AACGTT AA^CGTT AhdI 1013 GACGGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 1584 GATCC GGATC(4/5) AlwNI 500 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 878 AGCGCT AGC^GCT Asp700I 406 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1013 GACGGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 768 GGACC G^G[AT]CC AviII 297 TGCGCA TGC^GCA BalI 1439 TGGCCA TGG^CCA BanI 748 GGCGCC G^G[CT][GA]CC BbeI 748 GGCGCC GGCGC^C BbrPI 894 CACGTG CAC^GTG BbvCI 125 CCTCAGC CCTCAGC(-5/-2) BciVI 1179 GGATAC GTATCC(6/5) BclI 215 TGATCA T^GATCA BglII 1566 AGATCT A^GATCT BsaBI 1683 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 325 GAATGC GAATGC(1/-1) BspHI 522 TCATGA T^CATGA DraI 1333 TTTAAA TTT^AAA DraIII 792 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 225 GACCATGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1013 GACGGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 878 AGCGCT AGC^GCT EcoT14I 1694 CCATGG C^C[AT][AT]GG EcoT22I 327 ATGCAT ATGCA^T FauI 55 CCCGC CCCGC(4/6) FbaI 215 TGATCA T^GATCA FspI 297 TGCGCA TGC^GCA HincII 1025 GTCGAC GT[CT]^[GA]AC HindII 1025 GTCGAC GT[CT]^[GA]AC KasI 748 GGCGCC G^GCGCC KspI 77 CCGCGG CCGC^GG MamI 1683 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1343 CAATTG C^AATTG MluNI 1439 TGGCCA TGG^CCA MscI 1439 TGGCCA TGG^CCA MunI 1343 CAATTG C^AATTG NaeI 58 GCCGGC GCC^GGC NarI 748 GGCGCC GG^CGCC NcoI 1694 CCATGG C^CATGG NgoMIV 58 GCCGGC G^CCGGC NlaIV 748 GGCGCC GG[ACGT]^[ACGT]CC NsiI 327 ATGCAT ATGCA^T PaeR7I 1707 CTCGAG C^TCGAG PflMI 600 CCAATATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 894 CACGTG CAC^GTG PmlI 894 CACGTG CAC^GTG Psp1406I 840 AACGTT AA^CGTT RcaI 522 TCATGA T^CATGA SacII 77 CCGCGG CCGC^GG SalI 1025 GTCGAC G^TCGAC SfoI 748 GGCGCC GGC^GCC SmlI 1707 CTCGAG C^T[CT][GA]AG StyI 1694 CCATGG C^C[AT][AT]GG Van91I 600 CCAATATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1362 TCTAGA T^CTAGA XhoI 1707 CTCGAG C^TCGAG XmnI 406 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AccI 872 GTCTAC GT^[AC][GT]AC 1025 GTCGAC AccII 36 CGCG CG^CG 78 CGCG AcyI 748 GGCGCC G[GA]^CG[CT]C 1603 GACGTC AfaI 912 GTAC GT^AC 1159 GTAC AflIII 895 ACGTGT A^C[GA][CT]GT 1061 ACACGT AvaI 925 CCCGAG C^[CT]CG[GA]G 1707 CTCGAG BbsI 1323 GAAGAC GAAGAC(2/6) 276 GTCTTC BcnI 83 CCGGG CC^[GC]GG 1658 CCCGG BpuAI 1323 GAAGAC GAAGAC(2/6) 276 GTCTTC BsaAI 894 CACGTG [CT]AC^GT[GA] 1062 CACGTA BsaHI 748 GGCGCC G[GA]^CG[CT]C 1603 GACGTC BsiEI 80 CGGCCG CG[GA][CT]^CG 192 CGGCCG BsoBI 925 CCCGAG C^[CT]CG[GA]G 1707 CTCGAG BsrDI 511 GCAATG GCAATG(2/0) 662 GCAATG BsrFI 58 GCCGGC [GA]^CCGG[CT] 908 GCCGGT BstF5I 831 CATCC GGATG(2/0) 1006 GGATG BstUI 36 CGCG CG^CG 78 CGCG BstYI 1566 AGATCT [GA]^GATC[CT] 1583 AGATCC Cfr10I 58 GCCGGC [GA]^CCGG[CT] 908 GCCGGT Cfr13I 768 GGACC G^G[ACGT]CC 1755 GGGCC DsaI 77 CCGCGG C^C[GA][CT]GG 1694 CCATGG EagI 80 CGGCCG C^GGCCG 192 CGGCCG EarI 1719 CTCTTC CTCTTC(1/4) 1192 GAAGAG EclXI 80 CGGCCG C^GGCCG 192 CGGCCG Eco52I 80 CGGCCG C^GGCCG 192 CGGCCG FokI 831 CATCC GGATG(9/13) 1006 GGATG Hin1I 748 GGCGCC G[GA]^CG[CT]C 1603 GACGTC Ksp632I 1719 CTCTTC CTCTTC(1/4) 1192 GAAGAG MflI 1566 AGATCT [GA]^GATC[CT] 1583 AGATCC MvnI 36 CGCG CG^CG 78 CGCG NciI 83 CCGGG CC^[GC]GG 1658 CCCGG NspI 658 ACATGC [GA]CATG^[CT] 1029 ACATGC PstI 1510 CTGCAG CTGCA^G 1570 CTGCAG RsaI 912 GTAC GT^AC 1159 GTAC SacI 31 GAGCTC GAGCT^C 1456 GAGCTC Sau96I 768 GGACC G^G[ACGT]CC 1755 GGGCC XcmI 596 CCAACCAATATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 999 CCACATAGGATGTGG XhoII 1566 AGATCT [GA]^GATC[CT] 1583 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AluI Alw44I ApaLI AspHI BanII BbvI BfaI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I CfoI DdeI DpnII EaeI EcoRII Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII PleI PvuII Sau3AI ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I