THOC3 ORF clone FHC22392

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC22392

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Clone info.

Species

human

Clone Name

pFN21AE5452 Sequence (with vector) Restriction Map

Product ID

FHC22392

ORF Sequence

DNA sequence (1053 bp)
Protein sequence (351 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5452

Symbol

THOC3

Alias : THO3, hTREX45

Description

THO complex 3

Original Clone ID

oc95452

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1053 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 351 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4397.1 3.1e-114 100.0 THOC3 CCDS3012.1 3.4e-11 26.3 WDR5B CCDS55859.1 4.2e-11 29.9 POC1B CCDS31869.1 4.6e-11 29.9 POC1B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR020472 70 84 PR00320 G-protein beta WD-40 repeat IPR020472 115 129 PR00320 G-protein beta WD-40 repeat IPR020472 239 253 PR00320 G-protein beta WD-40 repeat HMMPfam IPR019781 48 81 PF00400 WD40 repeat IPR019781 95 128 PF00400 WD40 repeat IPR011659 138 157 PF07676 WD40-like Beta Propeller IPR019781 215 252 PF00400 WD40 repeat IPR019781 268 290 PF00400 WD40 repeat IPR019781 311 329 PF00400 WD40 repeat HMMSmart IPR001680 44 83 SM00320 WD40 repeat IPR001680 88 128 SM00320 WD40 repeat IPR001680 131 169 SM00320 WD40 repeat IPR001680 213 252 SM00320 WD40 repeat IPR001680 255 294 SM00320 WD40 repeat IPR001680 297 346 SM00320 WD40 repeat ProfileScan IPR019782 51 92 PS50082 WD40 repeat 2 IPR017986 51 303 PS50294 WD40-repeat-containing domain IPR019782 95 137 PS50082 WD40 repeat 2 IPR019782 220 254 PS50082 WD40 repeat 2
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_115737 351 606929 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5452 Download

>KIEE5452  1053 bp
ATGGCGGTCCCCGCTGCAGCCATGGGGCCCTCGGCGTTGGGCCAGAGCGGCCCCGGCTCG
ATGGCCCCGTGGTGCTCAGTGAGCAGCGGCCCGTCGCGCTACGTGCTTGGGATGCAGGAG
CTGTTCCGGGGCCACAGCAAGACGCGCGAGTTCCTGGCGCACAGCGCCAAGGTGCACTCG
GTGGCCTGGAGTTGCGACGGGCGTCGCCTAGCCTCGGGGTCCTTCGACAAGACGGCCAGC
GTCTTCTTGCTGGAGAAGGACCGGTTGGTCAAAGAAAACAATTATCGGGGACATGGGGAT
AGTGTGGACCAGCTTTGTTGGCATCCAAGTAATCCTGACCTATTTGTTACGGCGTCTGGA
GATAAAACCATTCGCATCTGGGATGTGAGGACTACAAAATGCATTGCCACTGTGAACACT
AAAGGGGAGAACATTAATATCTGCTGGAGTCCTGATGGGCAGACCATTGCTGTAGGCAAC
AAGGATGATGTGGTGACCTTTATTGATGCCAAGACACACCGTTCCAAAGCAGAAGAGCAG
TTCAAGTTCGAGGTCAACGAAATCTCCTGGAACAATGACAATAATATGTTCTTCCTGACA
AATGGCAATGGTTGTATCAACATCCTCAGCTACCCAGAACTGAAGCCTGTGCAGTCCATC
AACGCCCATCCTTCCAACTGCATCTGTATCAAGTTTGACCCCATGGGGAAGTACTTTGCC
ACAGGAAGTGCAGATGCTTTGGTCAGCCTCTGGGATGTGGATGAGTTAGTGTGTGTTCGG
TGCTTTTCCAGGCTGGATTGGCCTGTAAGAACCCTCAGTTTCAGCCATGATGGGAAAATG
CTGGCGTCAGCATCGGAAGATCATTTTATTGACATTGCTGAAGTGGAGACAGGGGACAAA
CTATGGGAGGTACAGTGTGAGTCTCCGACCTTCACAGTGGCGTGGCACCCCAAAAGGCCT
CTGCTGGCATTTGCCTGTGATGACAAAGACGGCAAATATGACAGCAGCCGGGAAGCCGGA
ACTGTGAAGCTGTTTGGGCTTCCTAATGATTCT

ORF Protein

Cloned ORF protein sequence for pFN21AE5452 Download

>KIEE5452  351 aa
MAVPAAAMGPSALGQSGPGSMAPWCSVSSGPSRYVLGMQELFRGHSKTREFLAHSAKVHS
VAWSCDGRRLASGSFDKTASVFLLEKDRLVKENNYRGHGDSVDQLCWHPSNPDLFVTASG
DKTIRIWDVRTTKCIATVNTKGENINICWSPDGQTIAVGNKDDVVTFIDAKTHRSKAEEQ
FKFEVNEISWNNDNNMFFLTNGNGCINILSYPELKPVQSINAHPSNCICIKFDPMGKYFA
TGSADALVSLWDVDELVCVRCFSRLDWPVRTLSFSHDGKMLASASEDHFIDIAEVETGDK
LWEVQCESPTFTVAWHPKRPLLAFACDDKDGKYDSSREAGTVKLFGLPNDS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5452 Download

>pFN21AE5452  5758 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGTCCCCGCTGCAGCCATGGGGCCCTCGGC
GTTGGGCCAGAGCGGCCCCGGCTCGATGGCCCCGTGGTGCTCAGTGAGCAGCGGCCCGTC
GCGCTACGTGCTTGGGATGCAGGAGCTGTTCCGGGGCCACAGCAAGACGCGCGAGTTCCT
GGCGCACAGCGCCAAGGTGCACTCGGTGGCCTGGAGTTGCGACGGGCGTCGCCTAGCCTC
GGGGTCCTTCGACAAGACGGCCAGCGTCTTCTTGCTGGAGAAGGACCGGTTGGTCAAAGA
AAACAATTATCGGGGACATGGGGATAGTGTGGACCAGCTTTGTTGGCATCCAAGTAATCC
TGACCTATTTGTTACGGCGTCTGGAGATAAAACCATTCGCATCTGGGATGTGAGGACTAC
AAAATGCATTGCCACTGTGAACACTAAAGGGGAGAACATTAATATCTGCTGGAGTCCTGA
TGGGCAGACCATTGCTGTAGGCAACAAGGATGATGTGGTGACCTTTATTGATGCCAAGAC
ACACCGTTCCAAAGCAGAAGAGCAGTTCAAGTTCGAGGTCAACGAAATCTCCTGGAACAA
TGACAATAATATGTTCTTCCTGACAAATGGCAATGGTTGTATCAACATCCTCAGCTACCC
AGAACTGAAGCCTGTGCAGTCCATCAACGCCCATCCTTCCAACTGCATCTGTATCAAGTT
TGACCCCATGGGGAAGTACTTTGCCACAGGAAGTGCAGATGCTTTGGTCAGCCTCTGGGA
TGTGGATGAGTTAGTGTGTGTTCGGTGCTTTTCCAGGCTGGATTGGCCTGTAAGAACCCT
CAGTTTCAGCCATGATGGGAAAATGCTGGCGTCAGCATCGGAAGATCATTTTATTGACAT
TGCTGAAGTGGAGACAGGGGACAAACTATGGGAGGTACAGTGTGAGTCTCCGACCTTCAC
AGTGGCGTGGCACCCCAAAAGGCCTCTGCTGGCATTTGCCTGTGATGACAAAGACGGCAA
ATATGACAGCAGCCGGGAAGCCGGAACTGTGAAGCTGTTTGGGCTTCCTAATGATTCTGT
TTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAG
CTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGC
AATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGAT
GAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGT
GATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT
TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAA
AACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCC
CTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTG
GCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGA
ACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAA
CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT
GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG
CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTG
GATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG
AGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG
CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACA
GAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATG
AGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACC
GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTG
AATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACG
TTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC
TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG
TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG
GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
TTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATAC
GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAA
AGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG
ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA
GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC
TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC
GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC
CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG
TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT
ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGA
CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT
CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG
CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCT
TCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTG
GGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTG
TACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGT
TTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTT
TTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCC
ATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT