################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AhdI AlwI AocI Aor51HI ApoI AscI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV FbaI FseI FspI HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI SexAI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AgeI 260 ACCGGT A^CCGGT Alw44I 172 GTGCAC G^TGCAC ApaI 25 GGGCCC GGGCC^C ApaLI 172 GTGCAC G^TGCAC AseI 433 ATTAAT AT^TAAT AsnI 433 ATTAAT AT^TAAT AvaI 213 CTCGGG C^[CT]CG[GA]G BanI 944 GGCACC G^G[CT][GA]CC BanII 25 GGGCCC G[GA]GC[CT]^C BbsI 241 GTCTTC GAAGAC(2/6) BbvCI 624 CCTCAGC CCTCAGC(-5/-2) BfaI 208 CTAG C^TAG BglI 41 GCCAGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 241 GTCTTC GAAGAC(2/6) BsaAI 100 TACGTG [CT]AC^GT[GA] BsaWI 260 ACCGGT [AT]^CCGG[AT] BsiYI 207 CCTAGCCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 207 CCTAGCCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsoBI 213 CTCGGG C^[CT]CG[GA]G BsrBI 45 GAGCGG CCGCTC(-3/-3) BsrFI 260 ACCGGT [GA]^CCGG[CT] BstEII 492 GGTGACC G^GT[ACGT]ACC BstPI 492 GGTGACC G^GT[ACGT]ACC Cfr10I 260 ACCGGT [GA]^CCGG[CT] DpnII 859 GATC ^GATC DraIII 175 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 259 GACCGGTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 233 CGGCCA [CT]^GGCC[GA] EarI 532 GAAGAG CTCTTC(1/4) EcoO65I 492 GGTGACC G^GT[ACGT]ACC EcoT22I 399 ATGCAT ATGCA^T FauI 10 CCCGC CCCGC(4/6) HaeII 163 AGCGCC [GA]GCGC^[CT] HincII 553 GTCAAC GT[CT]^[GA]AC HindII 553 GTCAAC GT[CT]^[GA]AC HphI 492 GGTGA GGTGA(8/7) Ksp632I 532 GAAGAG CTCTTC(1/4) MaeI 208 CTAG C^TAG MaeII 101 ACGT A^CGT MboI 859 GATC ^GATC MseI 434 TTAA T^TAA NdeII 859 GATC ^GATC NsiI 399 ATGCAT ATGCA^T PinAI 260 ACCGGT A^CCGGT PpuMI 217 GGGTCCT [GA]G^G[AT]CC[CT] PshAI 196 GACGGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 433 ATTAAT AT^TAAT PstI 14 CTGCAG CTGCA^G SapI 532 GAAGAGC GCTCTTC(1/4) Sau3AI 859 GATC ^GATC ScaI 710 AGTACT AGT^ACT SfiI 40 GGCCAGAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 955 AGGCCT AGG^CCT TfiI 1048 GATTC G^A[AT]TC Tru9I 434 TTAA T^TAA Tsp45I 493 GTGAC ^GT[GC]AC Tsp509I 280 AATT ^AATT ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AfaI 711 GTAC GT^AC 910 GTAC AlwNI 115 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 744 CAGCCTCTG AspHI 72 GTGCTC G[AT]GC[AT]^C 172 GTGCAC BsgI 649 GTGCAG GTGCAG(16/14) 728 GTGCAG BsiHKAI 72 GTGCTC G[AT]GC[AT]^C 172 GTGCAC BsmAI 886 GAGAC GTCTC(1/5) 921 GTCTC MaeIII 346 GTTAC ^GT[ACGT]AC 493 GTGAC MspA1I 11 CCGCTG C[AC]G^C[GT]G 84 CAGCGG NcoI 20 CCATGG C^CATGG 701 CCATGG PleI 447 GAGTC GAGTC(4/5) 919 GAGTC RsaI 711 GTAC GT^AC 910 GTAC SfcI 14 CTGCAG C^T[GA][CT]AG 470 CTGTAG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AcyI AluI AvaII BbvI BcnI BmyI BpmI BsaHI BsaJI BsmFI Bsp1286I BsrDI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI MboII MnlI MspI MvaI MvnI MwoI NciI NlaIII NlaIV Sau96I ScrFI SfaNI StyI TaqI TseI TspRI TthHB8I