(Linear) (Six-base) MAPSORT of: KIEE5452.seq Check: 3948 from: 1 to: 1053 KIEE5452.orf With 182 enzymes: SgfI * January 29, 2014 12:01 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 661 899 1053 Size: 661 238 154 Fragments arranged by size: 661 238 154 AgeI A'CCGG_T Cuts at: 0 260 1053 Size: 260 793 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 482 1053 Size: 482 571 AlwNI CAG_nnn'CTG Cuts at: 0 120 749 1053 Size: 120 629 304 Fragments arranged by size: 629 304 120 ApaI G_GGCC'C Cuts at: 0 29 1053 Size: 29 1024 ApaLI G'TGCA_C Cuts at: 0 172 1053 Size: 172 881 AseI AT'TA_AT Cuts at: 0 434 1053 Size: 434 619 AvaI C'yCGr_G Cuts at: 0 213 1053 Size: 213 840 BanI G'GyrC_C Cuts at: 0 944 1053 Size: 944 109 BanII G_rGCy'C Cuts at: 0 29 1053 Size: 29 1024 BbsI GAAGACnn'nnnn_ Cuts at: 0 234 1053 Size: 234 819 BbvCI CC'TCA_GC Cuts at: 0 625 1053 Size: 625 428 BfrBI ATG'CAT Cuts at: 0 401 1053 Size: 401 652 BglI GCCn_nnn'nGGC Cuts at: 0 47 1053 Size: 47 1006 Bme1580I G_kGCm'C Cuts at: 0 29 176 1053 Size: 29 147 877 Fragments arranged by size: 877 147 29 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 207 271 377 465 1053 Size: 207 64 106 88 588 Fragments arranged by size: 588 207 106 88 64 Bpu10I CC'TnA_GC Cuts at: 0 625 1053 Size: 625 428 BsaAI yAC'GTr Cuts at: 0 102 1053 Size: 102 951 BsaHI Gr'CG_yC Cuts at: 0 201 352 844 1053 Size: 201 151 492 209 Fragments arranged by size: 492 209 201 151 BsaWI w'CCGG_w Cuts at: 0 260 1053 Size: 260 793 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 547 577 898 928 1053 Size: 547 30 321 30 125 Fragments arranged by size: 547 321 125 30 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 670 749 1053 Size: 670 79 304 Fragments arranged by size: 670 304 79 BsiHKAI G_wGCw'C Cuts at: 0 76 176 1053 Size: 76 100 877 Fragments arranged by size: 877 100 76 Bsp1286I G_dGCh'C Cuts at: 0 29 76 176 1053 Size: 29 47 100 877 Fragments arranged by size: 877 100 47 29 BsrBI CCG'CTC Cuts at: 0 47 1053 Size: 47 1006 BsrDI GCAATG_nn' Cuts at: 0 401 464 612 872 1053 Size: 401 63 148 260 181 Fragments arranged by size: 401 260 181 148 63 BsrFI r'CCGG_y Cuts at: 0 260 1053 Size: 260 793 BstEII G'GTnAC_C Cuts at: 0 492 1053 Size: 492 561 BtgI C'CryG_G Cuts at: 0 20 67 701 1053 Size: 20 47 634 352 Fragments arranged by size: 634 352 47 20 DraIII CAC_nnn'GTG Cuts at: 0 180 1053 Size: 180 873 DrdI GACnn_nn'nnGTC Cuts at: 0 265 1053 Size: 265 788 EaeI y'GGCC_r Cuts at: 0 233 1053 Size: 233 820 EarI CTCTTCn'nnn_ Cuts at: 0 527 1053 Size: 527 526 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 207 271 377 465 661 899 1053 Size: 207 64 106 88 196 238 154 Fragments arranged by size: 238 207 196 154 106 88 64 EcoO109I rG'GnC_Cy Cuts at: 0 25 26 218 1053 Size: 25 1 192 835 Fragments arranged by size: 835 192 25 1 HaeII r_GCGC'y Cuts at: 0 167 1053 Size: 167 886 HincII GTy'rAC Cuts at: 0 555 1053 Size: 555 498 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 698 949 1053 Size: 698 251 104 Fragments arranged by size: 698 251 104 MspA1I CmG'CkG Cuts at: 0 13 86 1053 Size: 13 73 967 Fragments arranged by size: 967 73 13 NcoI C'CATG_G Cuts at: 0 20 701 1053 Size: 20 681 352 Fragments arranged by size: 681 352 20 NsiI A_TGCA'T Cuts at: 0 403 1053 Size: 403 650 PfoI T'CCnGG_A Cuts at: 0 565 1053 Size: 565 488 PpuMI rG'GwC_Cy Cuts at: 0 218 1053 Size: 218 835 PshAI GACnn'nnGTC Cuts at: 0 200 1053 Size: 200 853 PspOMI G'GGCC_C Cuts at: 0 25 1053 Size: 25 1028 PstI C_TGCA'G Cuts at: 0 18 1053 Size: 18 1035 SapI GCTCTTCn'nnn_ Cuts at: 0 527 1053 Size: 527 526 ScaI AGT'ACT Cuts at: 0 712 1053 Size: 712 341 SfcI C'TryA_G Cuts at: 0 14 470 1053 Size: 14 456 583 Fragments arranged by size: 583 456 14 SfiI GGCCn_nnn'nGGCC Cuts at: 0 47 1053 Size: 47 1006 StuI AGG'CCT Cuts at: 0 957 1053 Size: 957 96 StyI C'CwwG_G Cuts at: 0 20 167 701 1053 Size: 20 147 534 352 Fragments arranged by size: 534 352 147 20 TatI w'GTAC_w Cuts at: 0 710 1053 Size: 710 343 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 898 930 1053 Size: 898 32 123 Fragments arranged by size: 898 123 32 Enzymes that do cut: AcuI AgeI AlfI AlwNI ApaI ApaLI AseI AvaI BanI BanII BbsI BbvCI BfrBI BglI Bme1580I BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BsgI BsiHKAI Bsp1286I BsrBI BsrDI BsrFI BstEII BtgI DraIII DrdI EaeI EarI Eco57MI EcoO109I HaeII HincII MmeI MspA1I NcoI NsiI PfoI PpuMI PshAI PspOMI PstI SapI ScaI SfcI SfiI StuI StyI TatI TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AhdI AleI AloI ApoI AscI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI BseRI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI KasI KpnI MfeI MluI MscI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI SexAI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI ZraI