(Circular) (Six-base) MAPSORT of: pFN21AE5452.seq Check: 1623 from: 1 to: 5758 pFN21AE5452 5758 bp With 182 enzymes: SgfI * January 30, 2014 17:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3568 278 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3101 1937 Size: 1164 4594 Acc65I G'GTAC_C Cuts at: 3079 3079 Size: 5758 AclI AA'CG_TT Cuts at: 1563 3885 4258 1563 Size: 2322 373 3063 Fragments arranged by size: 3063 2322 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2666 2904 3821 5205 5473 1632 Size: 1034 238 917 1384 268 1917 Fragments arranged by size: 1917 1384 1034 917 268 238 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5561 AflIII A'CryG_T Cuts at: 1203 4657 5634 1203 Size: 3454 977 1327 Fragments arranged by size: 3454 1327 977 AgeI A'CCGG_T Cuts at: 2265 4589 2265 Size: 2324 3434 AhdI GACnn_n'nnGTC Cuts at: 4487 4487 Size: 5758 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5758 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2487 1184 Size: 1303 4455 AlwNI CAG_nnn'CTG Cuts at: 2125 2754 3501 5073 2125 Size: 629 747 1572 2810 Fragments arranged by size: 2810 1572 747 629 ApaI G_GGCC'C Cuts at: 2034 2034 Size: 5758 ApaLI G'TGCA_C Cuts at: 2177 3815 4971 2177 Size: 1638 1156 2964 Fragments arranged by size: 2964 1638 1156 ApoI r'AATT_y Cuts at: 1493 3067 3293 3445 5554 1493 Size: 1574 226 152 2109 1697 Fragments arranged by size: 2109 1697 1574 226 152 AseI AT'TA_AT Cuts at: 160 1032 2439 4312 160 Size: 872 1407 1873 1606 Fragments arranged by size: 1873 1606 1407 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 AvaI C'yCGr_G Cuts at: 1870 1943 2218 3083 1870 Size: 73 275 865 4545 Fragments arranged by size: 4545 865 275 73 BamHI G'GATC_C Cuts at: 3088 3455 3088 Size: 367 5391 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2949 3079 4534 618 Size: 325 217 627 1162 130 1455 1842 Fragments arranged by size: 1842 1455 1162 627 325 217 130 BanII G_rGCy'C Cuts at: 1391 2034 3077 1391 Size: 643 1043 4072 Fragments arranged by size: 4072 1043 643 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2239 928 Size: 971 340 4447 Fragments arranged by size: 4447 971 340 BbvCI CC'TCA_GC Cuts at: 2630 2630 Size: 5758 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3949 3983 1903 Size: 34 2012 34 3678 Fragments arranged by size: 3678 2012 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3650 4860 3650 Size: 1210 4548 BclI T'GATC_A Cuts at: 1548 1548 Size: 5758 BfrBI ATG'CAT Cuts at: 2406 2406 Size: 5758 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2052 4369 136 Size: 107 122 71 631 605 380 2317 1525 Fragments arranged by size: 2317 1525 631 605 380 122 107 71 BglII A'GATC_T Cuts at: 5753 5753 Size: 5758 BlpI GC'TnA_GC Cuts at: 3175 3175 Size: 5758 Bme1580I G_kGCm'C Cuts at: 1790 2034 2181 3819 4975 1790 Size: 244 147 1638 1156 2573 Fragments arranged by size: 2573 1638 1156 244 147 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5758 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3489 4447 442 Size: 416 53 83 399 24 2072 958 1753 Fragments arranged by size: 2072 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5758 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2212 2276 2382 2470 4418 1239 Size: 973 64 106 88 1948 2579 Fragments arranged by size: 2579 1948 973 106 88 64 Bpu10I CC'TnA_GC Cuts at: 2630 2630 Size: 5758 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3883 4748 5046 3883 Size: 865 298 4595 Fragments arranged by size: 4595 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4421 882 Size: 617 2922 2219 Fragments arranged by size: 2922 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2107 3549 493 Size: 711 903 1442 2702 Fragments arranged by size: 2702 1442 903 711 BsaBI GATnn'nnATC Cuts at: 5752 5752 Size: 5758 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2206 2357 2849 3565 Size: 53 83 186 936 673 151 492 716 Cuts at: 3565 3947 4582 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 716 673 635 492 382 186 151 83 53 BsaWI w'CCGG_w Cuts at: 2265 4191 4589 4863 5010 5421 2265 Size: 1926 398 274 147 411 2602 Fragments arranged by size: 2602 1926 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2552 2582 2903 2933 1178 Size: 30 165 30 1149 30 321 30 4003 Fragments arranged by size: 4003 1149 321 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5758 BseYI C'CCAG_C Cuts at: 4961 5457 4961 Size: 496 5262 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2675 2754 1163 Size: 573 939 79 4167 Fragments arranged by size: 4167 939 573 79 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3208 3969 4997 1540 Size: 275 186 1207 761 1028 2301 Fragments arranged by size: 2301 1207 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2081 2181 3819 3904 4975 2081 Size: 100 1638 85 1071 2864 Fragments arranged by size: 2864 1638 1071 100 85 BsmI GAATG_Cn' Cuts at: 3269 3362 3269 Size: 93 5665 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2081 2181 3077 3819 3904 4975 Size: 399 244 47 100 896 742 85 1071 Cuts at: 4975 1391 Size: 2174 Fragments arranged by size: 2174 1071 896 742 399 244 100 85 47 BspEI T'CCGG_A Cuts at: 5421 5421 Size: 5758 BspHI T'CATG_A Cuts at: 3647 5377 3647 Size: 1730 4028 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3113 844 Size: 2269 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2052 3645 1394 Size: 429 229 1593 3507 Fragments arranged by size: 3507 1593 429 229 BsrDI GCAATG_nn' Cuts at: 65 1248 2406 2469 2617 2877 4253 4427 65 Size: 1183 1158 63 148 260 1376 174 1396 Fragments arranged by size: 1396 1376 1183 1158 260 174 148 63 BsrFI r'CCGG_y Cuts at: 1700 1810 2265 4402 4589 1700 Size: 110 455 2137 187 2869 Fragments arranged by size: 2869 2137 455 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5758 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5758 BssSI C'ACGA_G Cuts at: 3818 4830 3818 Size: 1012 4746 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5758 BstBI TT'CG_AA Cuts at: 4562 4562 Size: 5758 BstEII G'GTnAC_C Cuts at: 1184 2497 1184 Size: 1313 4445 BstYI r'GATC_y Cuts at: 1972 3088 3455 3840 3857 5309 5395 5407 5753 Size: 1116 367 385 17 1452 86 12 346 Cuts at: 5753 1972 Size: 1977 Fragments arranged by size: 1977 1452 1116 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2025 2072 2706 513 Size: 552 939 21 47 634 3565 Fragments arranged by size: 3565 939 634 552 47 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5019 Fragments arranged by size: 5019 683 56 BtsI GCAGTG_nn' Cuts at: 3274 4068 4088 3274 Size: 794 20 4944 Fragments arranged by size: 4944 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5758 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5723 DraI TTT'AAA Cuts at: 3062 3062 Size: 5758 DraIII CAC_nnn'GTG Cuts at: 2185 2185 Size: 5758 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2270 4765 809 Size: 507 954 2495 1802 Fragments arranged by size: 2495 1802 954 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2238 3205 4094 Size: 54 1425 50 275 18 408 967 889 Cuts at: 4094 5723 8 Size: 1629 43 Fragments arranged by size: 1629 1425 967 889 408 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3205 1537 Size: 275 1393 4090 Fragments arranged by size: 4090 1393 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2532 3688 1353 Size: 1179 1156 3423 Fragments arranged by size: 3423 1179 1156 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4345 4719 4865 387 Size: 1064 138 2756 374 146 1280 Fragments arranged by size: 2756 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2212 2276 2382 2470 2666 2904 3821 Size: 393 580 64 106 88 196 238 917 Cuts at: 3821 4418 5205 5473 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 917 787 597 580 393 268 238 196 106 88 64 EcoO109I rG'GnC_Cy Cuts at: 2030 2031 2223 2030 Size: 1 192 5565 Fragments arranged by size: 5565 192 1 EcoRI G'AATT_C Cuts at: 3067 3445 3067 Size: 378 5380 FspI TGC'GCA Cuts at: 4264 4264 Size: 5758 HaeII r_GCGC'y Cuts at: 1678 2172 4905 1678 Size: 494 2733 2531 Fragments arranged by size: 2733 2531 494 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4399 4431 4473 4505 1373 Size: 32 135 32 2827 32 42 32 2626 Fragments arranged by size: 2827 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2560 3102 3348 669 Size: 988 281 622 542 246 3079 Fragments arranged by size: 3079 988 622 542 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5758 HpaI GTT'AAC Cuts at: 3348 3348 Size: 5758 KpnI G_GTAC'C Cuts at: 3083 3083 Size: 5758 MfeI C'AATT_G Cuts at: 3357 3357 Size: 5758 MluI A'CGCG_T Cuts at: 5634 5634 Size: 5758 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2703 2954 4872 5056 1305 Size: 1398 251 1918 184 2007 Fragments arranged by size: 2007 1918 1398 251 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3936 Fragments arranged by size: 3936 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3716 4075 4234 518 Size: 648 2550 359 159 2042 Fragments arranged by size: 2550 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 2091 3174 3851 4999 5244 1606 Size: 412 73 1083 677 1148 245 2120 Fragments arranged by size: 2120 1148 1083 677 412 245 73 NcoI C'CATG_G Cuts at: 513 1065 2004 2025 2706 513 Size: 552 939 21 681 3565 Fragments arranged by size: 3565 939 681 552 21 NdeI CA'TA_TG Cuts at: 387 387 Size: 5758 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5758 NotI GC'GGCC_GC Cuts at: 3205 3205 Size: 5758 NsiI A_TGCA'T Cuts at: 2408 2408 Size: 5758 NspI r_CATG'y Cuts at: 1132 1229 3116 4661 1132 Size: 97 1887 1545 2229 Fragments arranged by size: 2229 1887 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5758 PciI A'CATG_T Cuts at: 4657 4657 Size: 5758 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5758 PfoI T'CCnGG_A Cuts at: 2570 2570 Size: 5758 PmeI GTTT'AAAC Cuts at: 3062 3062 Size: 5758 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3827 3859 5340 5372 3827 Size: 32 1481 32 4213 Fragments arranged by size: 4213 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2223 2223 Size: 5758 PshAI GACnn'nnGTC Cuts at: 1613 2205 1613 Size: 592 5166 PsiI TTA'TAA Cuts at: 3328 3328 Size: 5758 PspOMI G'GGCC_C Cuts at: 2030 2030 Size: 5758 PstI C_TGCA'G Cuts at: 830 2023 3110 830 Size: 1193 1087 3478 Fragments arranged by size: 3478 1193 1087 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5758 SalI G'TCGA_C Cuts at: 1936 3100 1936 Size: 1164 4594 SapI GCTCTTCn'nnn_ Cuts at: 2532 2532 Size: 5758 SbfI CC_TGCA'GG Cuts at: 3110 3110 Size: 5758 ScaI AGT'ACT Cuts at: 2717 4006 5718 2717 Size: 1289 1712 2757 Fragments arranged by size: 2757 1712 1289 SfcI C'TryA_G Cuts at: 826 1045 2019 2475 3106 4241 4922 5113 826 Size: 219 974 456 631 1135 681 191 1471 Fragments arranged by size: 1471 1135 974 681 631 456 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2052 2052 Size: 5758 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5758 SmaI CCC'GGG Cuts at: 1872 3085 1872 Size: 1213 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3862 4763 5025 820 Size: 197 926 1919 901 262 1553 Fragments arranged by size: 1919 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3549 493 Size: 3056 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5758 SphI G_CATG'C Cuts at: 1132 3116 1132 Size: 1984 3774 SspI AAT'ATT Cuts at: 5 52 3682 5 Size: 47 3630 2081 Fragments arranged by size: 3630 2081 47 StuI AGG'CCT Cuts at: 1517 2962 1517 Size: 1445 4313 StyI C'CwwG_G Cuts at: 513 1065 2004 2025 2172 2706 3197 513 Size: 552 939 21 147 534 491 3074 Fragments arranged by size: 3074 939 552 534 491 147 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3785 3802 3955 4140 4585 325 Size: 1255 2205 17 153 185 445 1498 Fragments arranged by size: 2205 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2715 4004 5716 Size: 274 80 33 51 1443 738 1289 1712 Cuts at: 5716 96 Size: 138 Fragments arranged by size: 1712 1443 1289 738 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2903 2935 5718 5750 2903 Size: 32 2783 32 2911 Fragments arranged by size: 2911 2783 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5758 XbaI T'CTAG_A Cuts at: 3094 3094 Size: 5758 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5758 XmaI C'CCGG_G Cuts at: 1870 3083 1870 Size: 1213 4545 XmnI GAAnn'nnTTC Cuts at: 3887 3887 Size: 5758 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3566 276 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI XcmI