FHC01498

Gene/Protein Characteristic Table for FHC01498

Species

human

Clone Name

pFN21AB5244 Sequence (with vector) Restriction Map

Product ID

FHC01498

Corresponding
Flexi Clone PID

FXC01498

Original Clone PID

ORK01498

ORF Sequence

DNA sequence (1068 bp)
Protein sequence (356 aa)

Linker Type

Flexi type

The extra Val codon is attached to the 3'-end of the ORF, because a Pme I site ...

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385071]

Gene

KIBB5244

Symbol

NEUROD1

Alias : BETA2, BHF-1, MODY6, NEUROD, bHLHa3

Description

neurogenic differentiation 1

Original Clone ID

bm02588

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1068 bp
Restriction map A Restriction map B

Features of the protein sequence

Description

Length: 356 aa
Result of homology search against nr database ( FASTA output, Multiple alignment) Entry Exp ID% Protein Source Q13562 1.5e-115 100.0 Neurogenic diff... Homo sapiens AAV38535 1.5e-115 100.0 neurogenic diff... synthetic construct BAA11558 2.7e-115 99.7 NeuroD [Homo sa... Homo sapiens AAV38536 2.7e-115 99.7 neurogenic diff... Homo sapiens XP_001497909 3.3e-115 99.7 similar to neur... Equus caballus The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001092 102 154 PF00010 Basic helix-loop-helix dimerisation region bHLH HMMSmart IPR001092 107 159 SM00353 Basic helix-loop-helix dimerisation region bHLH ProfileScan IPR001092 102 154 PS50888 Basic helix-loop-helix dimerisation region bHLH
Result of homology search against disease genes in OMIM database

(FASTA output, Multiple alignment)

H_id	H_len (aa)	OMIM_No.	ID (%)	Q_ali/Q_len (%)
NP_002491	356	125853,601724	100.0	100.0
NP_067014	331	611635	77.8	99.4
NP_006151	382	601725	69.5	98.6
NP_073565	337	611513	71.8	100.0