Restriction map for
pFN21AB5244
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AvrII
BamHI BbeI BbrPI BcgI BciVI
BclI BfrI BglI BglII BlnI
BsaAI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmFI BsmI BspDI
BspEI BspLU11I BsrDI BsrFI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI Hin1I HincII
HindII HindIII HpaI HphI KasI
KspI MaeIII MfeI MluI MroI
MslI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I StuI SwaI TfiI
Tsp45I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 694 GGTACC G^GTACC
AlwNI 403 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 929 GGGCCC GGGCC^C
Asp718I 694 GGTACC G^GTACC
AspHI 97 GAGCAC G[AT]GC[AT]^C
AspI 490 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 323 CCCGGG C^[CT]CG[GA]G
AviII 371 TGCGCA TGC^GCA
BalI 434 TGGCCA TGG^CCA
BbsI 715 GTCTTC GAAGAC(2/6)
BfaI 361 CTAG C^TAG
BlpI 828 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 828 GCTCAGC GC^T[ACGT]AGC
BpuAI 715 GTCTTC GAAGAC(2/6)
BsaBI 226 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 495 GGTCTC GGTCTC(1/5)
BsiHKAI 97 GAGCAC G[AT]GC[AT]^C
BsoBI 323 CCCGGG C^[CT]CG[GA]G
Bsp1407I 645 TGTACA T^GTACA
BsrGI 645 TGTACA T^GTACA
BssSI 100 CACGAG CACGAG(-5/-1)
BstF5I 225 GGATG GGATG(2/0)
CelII 828 GCTCAGC GC^T[ACGT]AGC
DraI 295 TTTAAA TTT^AAA
FokI 225 GGATG GGATG(9/13)
FspI 371 TGCGCA TGC^GCA
HapII 324 CCGG C^CGG
HpaII 324 CCGG C^CGG
KpnI 694 GGTACC GGTAC^C
MaeI 361 CTAG C^TAG
MaeII 722 ACGT A^CGT
MamI 226 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 434 TGGCCA TGG^CCA
MscI 434 TGGCCA TGG^CCA
MspA1I 962 CCGCTG C[AC]G^C[GT]G
MspI 324 CCGG C^CGG
NcoI 698 CCATGG C^CATGG
PflFI 490 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 70 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 911 CTGCAG CTGCA^G
SfaNI 833 GCATC GCATC(5/9)
SmaI 323 CCCGGG CCC^GGG
SphI 338 GCATGC GCATG^C
SspBI 645 TGTACA T^GTACA
SspI 994 AATATT AAT^ATT
Tth111I 490 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 536 CCACCACCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 323 CCCGGG C^CCGGG
XmaI 323 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 250 CGCG CG^CG
354 CGCG
Aor51HI 631 AGCGCT AGC^GCT
753 AGCGCT
BbvCI 40 CCTCAGC CCTCAGC(-5/-2)
816 CCTCAGC
BcnI 323 CCCGG CC^[GC]GG
324 CCGGG
BpmI 286 CTGGAG CTGGAG(16/14)
757 CTGGAG
BsmBI 246 GAGACG CGTCTC(1/5)
303 GAGACG
BspMI 368 ACCTGC ACCTGC(4/8)
617 ACCTGC
BsrBI 826 CCGCTC CCGCTC(-3/-3)
328 GAGCGG
BstUI 250 CGCG CG^CG
354 CGCG
BstXI 539 CCACCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
663 CCAGTCGCCTGG
BstYI 464 AGATCC [GA]^GATC[CT]
980 AGATCC
DsaI 698 CCATGG C^C[GA][CT]GG
939 CCACGG
EaeI 434 TGGCCA [CT]^GGCC[GA]
626 CGGCCA
Eco47III 631 AGCGCT AGC^GCT
753 AGCGCT
EcoNI 195 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
369 CCTGCGCAAGG
EcoT14I 48 CCAAGG C^C[AT][AT]GG
698 CCATGG
FauI 822 CCCGC CCCGC(4/6)
553 GCGGG
MflI 464 AGATCC [GA]^GATC[CT]
980 AGATCC
MseI 296 TTAA T^TAA
725 TTAA
MvnI 250 CGCG CG^CG
354 CGCG
NciI 323 CCCGG CC^[GC]GG
324 CCGGG
NspI 338 GCATGC [GA]CATG^[CT]
605 ACATGC
PpuMI 51 AGGTCCT [GA]G^G[AT]CC[CT]
191 AGGACCT
SexAI 491 ACCTGGT A^CC[AT]GGT
545 ACCTGGT
SfcI 744 CTACAG C^T[GA][CT]AG
911 CTGCAG
StyI 48 CCAAGG C^C[AT][AT]GG
698 CCATGG
Tru9I 296 TTAA T^TAA
725 TTAA
Tsp509I 299 AATT ^AATT
886 AATT
TspRI 155 CACTG CA[GC]TG[ACGT][ACGT]^
920 CACTG
XhoII 464 AGATCC [GA]^GATC[CT]
980 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BanII BbvI BmyI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BspHI BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII Fnu4HI HaeII
HaeIII HgaI HhaI HinP1I HinfI
ItaI Ksp632I MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
PleI RcaI RsaI Sau3AI Sau96I
ScrFI TaqI TseI TthHB8I