Restriction map for
pFN21AB5244
(Linear) (Six-base) MAPSORT of: KIBB5244.seq Check: 9759 from: 1 to: 1068
KIBB5244.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 626 1068
Size: 626 442
Acc65I G'GTAC_C
Cuts at: 0 694 1068
Size: 694 374
AfeI AGC'GCT
Cuts at: 0 633 755 1068
Size: 633 122 313
Fragments arranged by size:
633 313 122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 926 1068
Size: 397 529 142
Fragments arranged by size:
529 397 142
AlwNI CAG_nnn'CTG
Cuts at: 0 408 1068
Size: 408 660
ApaI G_GGCC'C
Cuts at: 0 933 1068
Size: 933 135
AvaI C'yCGr_G
Cuts at: 0 323 1068
Size: 323 745
BanI G'GyrC_C
Cuts at: 0 381 694 958 1068
Size: 381 313 264 110
Fragments arranged by size:
381 313 264 110
BanII G_rGCy'C
Cuts at: 0 456 764 933 1068
Size: 456 308 169 135
Fragments arranged by size:
456 308 169 135
BbsI GAAGACnn'nnnn_
Cuts at: 0 708 1068
Size: 708 360
BbvCI CC'TCA_GC
Cuts at: 0 41 817 1068
Size: 41 776 251
Fragments arranged by size:
776 251 41
BlpI GC'TnA_GC
Cuts at: 0 829 1068
Size: 829 239
Bme1580I G_kGCm'C
Cuts at: 0 933 1044 1068
Size: 933 111 24
Fragments arranged by size:
933 111 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 674 1068
Size: 674 394
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 168 1068
Size: 168 900
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 778 1068
Size: 307 471 290
Fragments arranged by size:
471 307 290
Bpu10I CC'TnA_GC
Cuts at: 0 41 474 590 817 1068
Size: 41 433 116 227 251
Fragments arranged by size:
433 251 227 116 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 501 1068
Size: 501 567
BsaBI GATnn'nnATC
Cuts at: 0 230 1068
Size: 230 838
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 481 511 640 670 1068
Size: 481 30 129 30 398
Fragments arranged by size:
481 398 129 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 160 187 190 217 235 1068
Size: 109 51 27 3 27 18 833
Fragments arranged by size:
833 109 51 27 27 18 3
BseYI C'CCAG_C
Cuts at: 0 1043 1068
Size: 1043 25
BsiHKAI G_wGCw'C
Cuts at: 0 101 1068
Size: 101 967
BsmBI CGTCTCn'nnnn_
Cuts at: 0 240 297 1068
Size: 240 57 771
Fragments arranged by size:
771 240 57
Bsp1286I G_dGCh'C
Cuts at: 0 101 456 764 933 1044 1068
Size: 101 355 308 169 111 24
Fragments arranged by size:
355 308 169 111 101 24
BspHI T'CATG_A
Cuts at: 0 1023 1034 1062 1068
Size: 1023 11 28 6
Fragments arranged by size:
1023 28 11 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 377 626 1068
Size: 377 249 442
Fragments arranged by size:
442 377 249
BsrBI CCG'CTC
Cuts at: 0 330 828 1068
Size: 330 498 240
Fragments arranged by size:
498 330 240
BsrGI T'GTAC_A
Cuts at: 0 645 1068
Size: 645 423
BssSI C'ACGA_G
Cuts at: 0 100 1068
Size: 100 968
BstXI CCAn_nnnn'nTGG
Cuts at: 0 546 670 1068
Size: 546 124 398
Fragments arranged by size:
546 398 124
BstYI r'GATC_y
Cuts at: 0 464 980 1068
Size: 464 516 88
Fragments arranged by size:
516 464 88
BtgI C'CryG_G
Cuts at: 0 698 939 1068
Size: 698 241 129
Fragments arranged by size:
698 241 129
DraI TTT'AAA
Cuts at: 0 297 1068
Size: 297 771
EaeI y'GGCC_r
Cuts at: 0 434 626 1068
Size: 434 192 442
Fragments arranged by size:
442 434 192
EarI CTCTTCn'nnn_
Cuts at: 0 194 206 212 1068
Size: 194 12 6 856
Fragments arranged by size:
856 194 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 674 722 858 1068
Size: 674 48 136 210
Fragments arranged by size:
674 210 136 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 778 1068
Size: 307 471 290
Fragments arranged by size:
471 307 290
EcoNI CCTnn'n_nnAGG
Cuts at: 0 199 373 1068
Size: 199 174 695
Fragments arranged by size:
695 199 174
EcoO109I rG'GnC_Cy
Cuts at: 0 52 192 929 1068
Size: 52 140 737 139
Fragments arranged by size:
737 140 139 52
FspI TGC'GCA
Cuts at: 0 373 1068
Size: 373 695
HaeII r_GCGC'y
Cuts at: 0 361 635 757 1068
Size: 361 274 122 311
Fragments arranged by size:
361 311 274 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 264 296 481 513 1068
Size: 264 32 185 32 555
Fragments arranged by size:
555 264 185 32 32
KpnI G_GTAC'C
Cuts at: 0 698 1068
Size: 698 370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 1068
Size: 440 628
MscI TGG'CCA
Cuts at: 0 436 1068
Size: 436 632
MspA1I CmG'CkG
Cuts at: 0 964 1068
Size: 964 104
NcoI C'CATG_G
Cuts at: 0 698 1068
Size: 698 370
NspI r_CATG'y
Cuts at: 0 342 609 1068
Size: 342 267 459
Fragments arranged by size:
459 342 267
PpuMI rG'GwC_Cy
Cuts at: 0 52 192 1068
Size: 52 140 876
Fragments arranged by size:
876 140 52
PshAI GACnn'nnGTC
Cuts at: 0 74 1068
Size: 74 994
PspOMI G'GGCC_C
Cuts at: 0 929 1068
Size: 929 139
PstI C_TGCA'G
Cuts at: 0 915 1068
Size: 915 153
SexAI A'CCwGG_T
Cuts at: 0 491 545 1068
Size: 491 54 523
Fragments arranged by size:
523 491 54
SfcI C'TryA_G
Cuts at: 0 744 911 1068
Size: 744 167 157
Fragments arranged by size:
744 167 157
SmaI CCC'GGG
Cuts at: 0 325 1068
Size: 325 743
SphI G_CATG'C
Cuts at: 0 342 1068
Size: 342 726
SspI AAT'ATT
Cuts at: 0 996 1068
Size: 996 72
StyI C'CwwG_G
Cuts at: 0 48 698 1068
Size: 48 650 370
Fragments arranged by size:
650 370 48
TatI w'GTAC_w
Cuts at: 0 645 1068
Size: 645 423
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 640 672 850 882 1068
Size: 640 32 178 32 186
Fragments arranged by size:
640 186 178 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 493 1068
Size: 493 575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 543 1068
Size: 543 525
XmaI C'CCGG_G
Cuts at: 0 323 1068
Size: 323 745
Enzymes that do cut:
AarI Acc65I AfeI AlfI AlwNI ApaI AvaI BanI
BanII BbsI BbvCI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BsaI BsaBI BsaXI BseRI BseYI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrGI BssSI BstXI BstYI
BtgI DraI EaeI EarI EciI Eco57MI EcoNI EcoO109I
FspI HaeII Hin4I KpnI MmeI MscI MspA1I NcoI
NspI PpuMI PshAI PspOMI PstI SexAI SfcI SmaI
SphI SspI StyI TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AcuI AflII AflIII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmtI BpuEI BsaAI BsaHI BsaWI BsgI
BsiEI BsiWI BsmI BspEI BsrDI BsrFI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI FseI
FspAI HincII HindIII HpaI KasI MfeI MluI MslI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SrfI StuI SwaI TaqII
XbaI XhoI XmnI ZraI