(Circular) (Six-base) MAPSORT of: pFN21AB5244.seq Check: 5688 from: 1 to: 5773 pFN21AB5244 5773 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2631 2631 Size: 5773 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3583 278 Size: 53 83 186 936 2047 2468 Fragments arranged by size: 2468 2047 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3116 1937 Size: 1179 4594 Acc65I G'GTAC_C Cuts at: 2699 3094 2699 Size: 395 5378 AclI AA'CG_TT Cuts at: 1563 3900 4273 1563 Size: 2337 373 3063 Fragments arranged by size: 3063 2337 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3836 5220 5488 1632 Size: 2204 1384 268 1917 Fragments arranged by size: 2204 1917 1384 268 AfeI AGC'GCT Cuts at: 2638 2760 2638 Size: 122 5651 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5576 AflIII A'CryG_T Cuts at: 1203 4672 5649 1203 Size: 3469 977 1327 Fragments arranged by size: 3469 1327 977 AgeI A'CCGG_T Cuts at: 4604 4604 Size: 5773 AhdI GACnn_n'nnGTC Cuts at: 4502 4502 Size: 5773 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5773 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2402 2931 1184 Size: 1218 529 4026 Fragments arranged by size: 4026 1218 529 AlwNI CAG_nnn'CTG Cuts at: 2413 3516 5088 2413 Size: 1103 1572 3098 Fragments arranged by size: 3098 1572 1103 ApaI G_GGCC'C Cuts at: 2938 2938 Size: 5773 ApaLI G'TGCA_C Cuts at: 3830 4986 3830 Size: 1156 4617 ApoI r'AATT_y Cuts at: 1493 3082 3308 3460 5569 1493 Size: 1589 226 152 2109 1697 Fragments arranged by size: 2109 1697 1589 226 152 AseI AT'TA_AT Cuts at: 160 1032 4327 160 Size: 872 3295 1606 Fragments arranged by size: 3295 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 AvaI C'yCGr_G Cuts at: 1870 1943 2328 3098 1870 Size: 73 385 770 4545 Fragments arranged by size: 4545 770 385 73 BamHI G'GATC_C Cuts at: 3103 3470 3103 Size: 367 5406 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2386 2699 2963 3094 4549 Size: 325 217 627 599 313 264 131 1455 Cuts at: 4549 618 Size: 1842 Fragments arranged by size: 1842 1455 627 599 325 313 264 217 131 BanII G_rGCy'C Cuts at: 1391 2461 2769 2938 3092 1391 Size: 1070 308 169 154 4072 Fragments arranged by size: 4072 1070 308 169 154 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2713 928 Size: 971 814 3988 Fragments arranged by size: 3988 971 814 BbvCI CC'TCA_GC Cuts at: 2046 2822 2046 Size: 776 4997 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3964 3998 1903 Size: 34 2027 34 3678 Fragments arranged by size: 3678 2027 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3665 4875 3665 Size: 1210 4563 BclI T'GATC_A Cuts at: 1548 1548 Size: 5773 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4384 136 Size: 107 122 71 631 605 2712 1525 Fragments arranged by size: 2712 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5768 5768 Size: 5773 BlpI GC'TnA_GC Cuts at: 2834 3190 2834 Size: 356 5417 Bme1580I G_kGCm'C Cuts at: 1790 2938 3049 3834 4990 1790 Size: 1148 111 785 1156 2573 Fragments arranged by size: 2573 1156 1148 785 111 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5773 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2679 3504 4462 Size: 416 53 83 399 24 1262 825 958 Cuts at: 4462 442 Size: 1753 Fragments arranged by size: 1753 1262 958 825 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5773 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2173 2173 Size: 5773 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2312 2783 4433 1239 Size: 1073 471 1650 2579 Fragments arranged by size: 2579 1650 1073 471 Bpu10I CC'TnA_GC Cuts at: 2046 2479 2595 2822 2046 Size: 433 116 227 4997 Fragments arranged by size: 4997 433 227 116 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3898 4763 5061 3898 Size: 865 298 4610 Fragments arranged by size: 4610 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2506 4436 882 Size: 617 1007 1930 2219 Fragments arranged by size: 2219 1930 1007 617 BsaAI yAC'GTr Cuts at: 493 1204 3564 493 Size: 711 2360 2702 Fragments arranged by size: 2702 2360 711 BsaBI GATnn'nnATC Cuts at: 2235 5767 2235 Size: 3532 2241 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3580 3962 4597 275 Size: 53 83 186 936 2047 382 635 1451 Fragments arranged by size: 2047 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4206 4604 4878 5025 5436 4206 Size: 398 274 147 411 4543 Fragments arranged by size: 4543 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2486 2516 2645 2675 1178 Size: 30 165 30 1083 30 129 30 4276 Fragments arranged by size: 4276 1083 165 129 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 2165 2192 2195 2222 2240 1186 Size: 928 51 27 3 27 18 4719 Fragments arranged by size: 4719 928 51 27 27 18 3 BseYI C'CCAG_C Cuts at: 3048 4976 5472 3048 Size: 1928 496 3349 Fragments arranged by size: 3349 1928 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5200 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3223 3984 5012 1540 Size: 275 186 1222 761 1028 2301 Fragments arranged by size: 2301 1222 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2106 3834 3919 4990 2106 Size: 1728 85 1071 2889 Fragments arranged by size: 2889 1728 1071 85 BsmI GAATG_Cn' Cuts at: 3284 3377 3284 Size: 93 5680 BsmBI CGTCTCn'nnnn_ Cuts at: 2245 2302 2245 Size: 57 5716 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2461 2769 2938 3049 3092 3834 Size: 399 316 355 308 169 111 43 742 Cuts at: 3834 3919 4990 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 355 316 308 169 111 85 43 BspEI T'CCGG_A Cuts at: 5436 5436 Size: 5773 BspHI T'CATG_A Cuts at: 3028 3039 3067 3662 5392 3028 Size: 11 28 595 1730 3409 Fragments arranged by size: 3409 1730 595 28 11 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2382 2631 3128 844 Size: 1538 249 497 3489 Fragments arranged by size: 3489 1538 497 249 BsrBI CCG'CTC Cuts at: 1394 1823 2335 2833 3660 1394 Size: 429 512 498 827 3507 Fragments arranged by size: 3507 827 512 498 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4268 4442 65 Size: 1183 3020 174 1396 Fragments arranged by size: 3020 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4417 4604 1700 Size: 110 2607 187 2869 Fragments arranged by size: 2869 2607 187 110 BsrGI T'GTAC_A Cuts at: 96 2650 96 Size: 2554 3219 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5773 BssSI C'ACGA_G Cuts at: 2105 3833 4845 2105 Size: 1728 1012 3033 Fragments arranged by size: 3033 1728 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5773 BstBI TT'CG_AA Cuts at: 4577 4577 Size: 5773 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5773 BstXI CCAn_nnnn'nTGG Cuts at: 2551 2675 2551 Size: 124 5649 BstYI r'GATC_y Cuts at: 1972 2469 2985 3103 3470 3855 3872 5324 5410 Size: 497 516 118 367 385 17 1452 86 Cuts at: 5410 5422 5768 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 516 497 385 367 346 118 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2703 2944 513 Size: 552 1638 241 3342 Fragments arranged by size: 3342 1638 552 241 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5034 Fragments arranged by size: 5034 683 56 BtsI GCAGTG_nn' Cuts at: 3289 4083 4103 3289 Size: 794 20 4959 Fragments arranged by size: 4959 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5773 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5738 DraI TTT'AAA Cuts at: 2302 3077 2302 Size: 775 4998 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4780 809 Size: 507 3464 1802 Fragments arranged by size: 3464 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2439 2631 3220 Size: 54 1425 50 275 18 609 192 589 Cuts at: 3220 4109 5738 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 609 589 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3220 1537 Size: 275 1408 4090 Fragments arranged by size: 4090 1408 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2199 2211 2217 3703 1353 Size: 846 12 6 1486 3423 Fragments arranged by size: 3423 1486 846 12 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2679 2727 2863 4360 4734 4880 Size: 1064 138 1090 48 136 1497 374 146 Cuts at: 4880 387 Size: 1280 Fragments arranged by size: 1497 1280 1090 1064 374 146 138 136 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2312 2783 3836 4433 5220 5488 1239 Size: 393 680 471 1053 597 787 268 1524 Fragments arranged by size: 1524 1053 787 680 597 471 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2204 2378 2204 Size: 174 5599 EcoO109I rG'GnC_Cy Cuts at: 2057 2197 2934 2057 Size: 140 737 4896 Fragments arranged by size: 4896 737 140 EcoRI G'AATT_C Cuts at: 3082 3460 3082 Size: 378 5395 FspI TGC'GCA Cuts at: 2378 4279 2378 Size: 1901 3872 HaeII r_GCGC'y Cuts at: 1678 2366 2640 2762 4920 1678 Size: 688 274 122 2158 2531 Fragments arranged by size: 2531 2158 688 274 122 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2269 2301 2486 2518 4414 Size: 32 135 32 697 32 185 32 1896 Cuts at: 4414 4446 4488 4520 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1896 697 185 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3117 3363 669 Size: 988 281 1179 246 3079 Fragments arranged by size: 3079 1179 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5773 HpaI GTT'AAC Cuts at: 3363 3363 Size: 5773 KpnI G_GTAC'C Cuts at: 2703 3098 2703 Size: 395 5378 MfeI C'AATT_G Cuts at: 3372 3372 Size: 5773 MluI A'CGCG_T Cuts at: 5649 5649 Size: 5773 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2445 4887 5071 1305 Size: 1140 2442 184 2007 Fragments arranged by size: 2442 2007 1140 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2441 10 Size: 54 1425 343 609 3342 Fragments arranged by size: 3342 1425 609 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3731 4090 4249 518 Size: 648 2565 359 159 2042 Fragments arranged by size: 2565 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2969 3189 3866 5014 5259 1606 Size: 1363 220 677 1148 245 2120 Fragments arranged by size: 2120 1363 1148 677 245 220 NcoI C'CATG_G Cuts at: 513 1065 2703 513 Size: 552 1638 3583 Fragments arranged by size: 3583 1638 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5773 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5773 NotI GC'GGCC_GC Cuts at: 3220 3220 Size: 5773 NspI r_CATG'y Cuts at: 1132 1229 2347 2614 3131 4676 1132 Size: 97 1118 267 517 1545 2229 Fragments arranged by size: 2229 1545 1118 517 267 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5773 PciI A'CATG_T Cuts at: 4672 4672 Size: 5773 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5773 PmeI GTTT'AAAC Cuts at: 3077 3077 Size: 5773 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3842 3874 5355 5387 3842 Size: 32 1481 32 4228 Fragments arranged by size: 4228 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2057 2197 2057 Size: 140 5633 PshAI GACnn'nnGTC Cuts at: 1613 2079 1613 Size: 466 5307 PsiI TTA'TAA Cuts at: 3343 3343 Size: 5773 PspOMI G'GGCC_C Cuts at: 2934 2934 Size: 5773 PstI C_TGCA'G Cuts at: 830 2920 3125 830 Size: 2090 205 3478 Fragments arranged by size: 3478 2090 205 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5773 SalI G'TCGA_C Cuts at: 1936 3115 1936 Size: 1179 4594 SbfI CC_TGCA'GG Cuts at: 3125 3125 Size: 5773 ScaI AGT'ACT Cuts at: 4021 5733 4021 Size: 1712 4061 SexAI A'CCwGG_T Cuts at: 2496 2550 2496 Size: 54 5719 SfcI C'TryA_G Cuts at: 826 1045 2749 2916 3121 4256 4937 5128 826 Size: 219 1704 167 205 1135 681 191 1471 Fragments arranged by size: 1704 1471 1135 681 219 205 191 167 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5773 SmaI CCC'GGG Cuts at: 1872 2330 3100 1872 Size: 458 770 4545 Fragments arranged by size: 4545 770 458 SmlI C'TyrA_G Cuts at: 820 1017 1943 3877 4778 5040 820 Size: 197 926 1934 901 262 1553 Fragments arranged by size: 1934 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3564 493 Size: 3071 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5773 SphI G_CATG'C Cuts at: 1132 2347 3131 1132 Size: 1215 784 3774 Fragments arranged by size: 3774 1215 784 SspI AAT'ATT Cuts at: 5 52 3001 3697 5 Size: 47 2949 696 2081 Fragments arranged by size: 2949 2081 696 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5773 StyI C'CwwG_G Cuts at: 513 1065 2053 2703 3212 513 Size: 552 988 650 509 3074 Fragments arranged by size: 3074 988 650 552 509 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3800 3817 3970 4155 4600 325 Size: 1255 2220 17 153 185 445 1498 Fragments arranged by size: 2220 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2650 4019 5731 Size: 274 80 33 51 1443 673 1369 1712 Cuts at: 5731 96 Size: 138 Fragments arranged by size: 1712 1443 1369 673 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2645 2677 2855 2887 5733 5765 2645 Size: 32 178 32 2846 32 2653 Fragments arranged by size: 2846 2653 178 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2498 1316 Size: 1182 4591 XbaI T'CTAG_A Cuts at: 3109 3109 Size: 5773 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2548 2548 Size: 5773 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5773 XmaI C'CCGG_G Cuts at: 1870 2328 3098 1870 Size: 458 770 4545 Fragments arranged by size: 4545 770 458 XmnI GAAnn'nnTTC Cuts at: 3902 3902 Size: 5773 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3581 276 Size: 53 83 186 936 2047 2468 Fragments arranged by size: 2468 2047 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI