HUGE

Gene/Protein Characteristic Table for KIAA1451

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00230
Accession No. :	AB040884
Description :	Oxysterol-binding protein-related protein 8.
HUGO Gene Name :
Clone Name :	bg00289 [Vector Info]
Flexi ORF Clone :	pF1KA1451
Source :	Human adult brain
Note :	We replaced fh12482, former representative clones for KIAA1451 with bg00289. (2002/5/10)

Features of the cloned DNA sequence	Description

Length: 6928 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 4107 bp

Genome contig ID gi89161190r_75169711
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
AATTTTTAAAAAGAGCAAAAAAAAAAAAAAAAAAA
Flanking genome sequence
(54430 - 54381)
----+----*----+----*----+----*----+----*----+----*
AAAGACTCAAGTCCTTATCCTACATACTGTGGAAAGTTCAGTGCCTTTCC

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 12 r 75224141 75477391 25 99.4 Perfect prediction

Features of the protein sequence	Description

Length: 939 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_509228	99.9	oxysterol-bindi...	Pan troglodytes
XP_001082052	99.1	similar to oxys...	Macaca mulatta
Q9BZF1	100.0	Oxysterol-bindi...	Homo sapiens
CAI46050	99.8	hypothetical pr...	Homo sapiens
CAH91148	99.7	hypothetical pr...	Pongo abelii

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB040967	6e-87	55.6	KIAA1534
AB018315	9.6e-16	26.9	KIAA0772
AB014604	2.6e-10	23.8	KIAA0704
AB051451	3.6e-10	25.3	KIAA1664

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR001849 199 315 PF00169 Pleckstrin-like

IPR000648 425 835 PF01237 Oxysterol-binding protein

HMMSmart IPR001849 199 317 SM00233 Pleckstrin-like

ProfileScan IPR001849 198 315 PS50003 Pleckstrin-like

ScanRegExp IPR000648 560 570 PS01013 Oxysterol-binding protein

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001849	199	315	PF00169	Pleckstrin-like
IPR000648	425	835	PF01237	Oxysterol-binding protein
HMMSmart	IPR001849	199	317	SM00233	Pleckstrin-like
ProfileScan	IPR001849	198	315	PS50003	Pleckstrin-like
ScanRegExp	IPR000648	560	570	PS01013	Oxysterol-binding protein

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 918 QKDYFIIFLLILLQVIINFMFK 939 PRIMARY 22

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	918	QKDYFIIFLLILLQVIINFMFK	939	PRIMARY	22

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: TAATCCTATACTTGGCGAGAC

Primer_r
: ACTTAGACTTAGCCAGGATAC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 12

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: CTTCACACATGGACCGTAAAC
Primer_r	: TACTAAATGGTGGATGATGGC
PCR product length	: 200 bp
PCR conditions	: 95 °C15 sec62 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	4107 bp
Genome contig ID	gi89161190r_75169711
PolyA signal sequence (None)	+--------+--------+----*----+---- AATTTTTAAAAAGAGCAAAAAAAAAAAAAAAAAAA
Flanking genome sequence (54430 - 54381)	----+--------+--------+--------+--------+----* AAAGACTCAAGTCCTTATCCTACATACTGTGGAAAGTTCAGTGCCTTTCC

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	12	r	75224141	75477391	25	99.4	Perfect prediction

Primer_f	: TAATCCTATACTTGGCGAGAC
Primer_r	: ACTTAGACTTAGCCAGGATAC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1451

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information