A Database of Human Unidentified Gene-Encoded Large Proteins Analyzed
by Kazusa Human cDNA Project


Thank you for your interest in our HUGE Protein Database

The HUGE protein database has been created to publicize the fruits of our Human cDNA project at the Kazusa DNA Research Institute. In this project, we plan to sequence and analyze long (>4 kb) human cDNAs and to establish methods by using the sequence data how to predict the primary structure of proteins of various biological activities. Currently, we focus on the analysis of cDNA clones encoding particularly large proteins (>50 kDa). The basic concept underlying our project and the strategies employed have been described elsewhere (Ohara et al., 1997). Our HUGE protein database contains various types of information derived from the predicted primary structure data of newly identified human proteins.

The HUGE protein database are expected to cover various sets of large human proteins of hitherto unidentified functions. They are likely to be involved in cellular structure/motility (such as cytoskeleton, membrane skeleton, and motor proteins), gene expression and nucleic acid metabolism, cell signaling/communication (such as cellular adhesion, signal transduction, channels, and receptors), and so on.


IMPORTANT NOTICE!!
Date: 18th December 2006
Regarding distribution of KIAA/FLJ cDNA clones:

Till now, we have distributed as many as 7,000 KIAA/FLJ cDNA clones free of charge to research communities all over the world. We are very happy to learn that a large number of valuable research papers have consequently been published that described functions associated with the gene(s) contained in the KIAA/FLJ clones distributed. Starting January 2007, however, we have to ask you to pay the shipping and handling costs on the per-clone basis (Japanese ¥ 15,000/clone or US$ 150/clone). This is partly because we have recently begun distribution of protein-expressing clones for value.

Please understand our new policy and refer to the new ordering procedure ( Japan || Other countries) when requesting our cDNA clone(s).

Other Human cDNA/ORF clone resources

Kazusa Mouse cDNA project

  • Rouge (ROdent Unidentified Gene-Encoded large proteins)
     Mouse homologues of KIAA and FLJ cDNA clones. Please visit Rouge.

REFERENCES

  • Kikuno, R., Nagase, T., Nakayama, M. et al., HUGE: a database for human KIAA proteins, a 2004 update integrating HUGEppi and ROUGE. Nucleic Acids Res. 32, D502-504, (2004).

  • Ohara, O., Nagase, T., Ishikawa, K.-I. et al., Construction and characterization of human brain cDNA libraries suitable for analysis of cDNA clones encoding relatively large proteins. DNA Res., 4, 53-59, (1997).

  • Bibliography

ACKNOWLEDGMENTS

We thank Takatsugu Hirokawa, Seah Boon-Chieng, and Shigeki Mitaku for allowing us to use the SOSUI program for the prediction of transmembrane helical regions.


DISCLAIMERS


RELATED DATABASES

How to obtain Kazusa clone(s) Japan || Othercountries
Send a message to office AT kazusa.or.jp