##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AspI AviII BbvCI BcgI
BlpI Bpu1102I BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrBI BssHII BssSI CelII ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FseI FspI
KpnI MluI MroI NaeI NgoMIV
NheI NotI NruI PacI PflFI
PinAI PmeI Psp1406I PvuI RsrII
SalI SbfI SexAI SfiI SgrAI
SmaI SpeI Sse8387I StuI SwaI
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccII 26 CGCG CG^CG
AcyI 21 GGCGCC G[GA]^CG[CT]C
Alw44I 6734 GTGCAC G^TGCAC
AocI 4378 CCTTAGG CC^T[ACGT]AGG
ApaLI 6734 GTGCAC G^TGCAC
Asp700I 4395 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 2223 CTCGAG C^[CT]CG[GA]G
BalI 6626 TGGCCA TGG^CCA
BamHI 845 GGATCC G^GATCC
BanI 21 GGCGCC G^G[CT][GA]CC
BanII 72 GAGCTC G[GA]GC[CT]^C
BbeI 21 GGCGCC GGCGC^C
BbsI 954 GTCTTC GAAGAC(2/6)
BcnI 18 CCCGG CC^[GC]GG
BglI 2506 GCCAACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 954 GTCTTC GAAGAC(2/6)
BsaHI 21 GGCGCC G[GA]^CG[CT]C
BsgI 107 CTGCAC GTGCAG(16/14)
BsoBI 2223 CTCGAG C^[CT]CG[GA]G
BsrDI 2143 GCAATG GCAATG(2/0)
Bst1107I 3095 GTATAC GTA^TAC
BstAPI 4082 GCAATTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2703 TTCGAA TT^CGAA
BstUI 26 CGCG CG^CG
BstXI 694 CCAAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3095 GTATAC GTA^TAC
Bsu36I 4378 CCTTAGG CC^T[ACGT]AGG
Eco81I 4378 CCTTAGG CC^T[ACGT]AGG
EcoRI 1881 GAATTC G^AATTC
EcoT22I 4959 ATGCAT ATGCA^T
FauI 59 CCCGC CCCGC(4/6)
Hin1I 21 GGCGCC G[GA]^CG[CT]C
KasI 21 GGCGCC G^GCGCC
KspI 25 CCGCGG CCGC^GG
MfeI 3102 CAATTG C^AATTG
MluNI 6626 TGGCCA TGG^CCA
MscI 6626 TGGCCA TGG^CCA
MunI 3102 CAATTG C^AATTG
MvnI 26 CGCG CG^CG
NarI 21 GGCGCC GG^CGCC
NciI 18 CCCGG CC^[GC]GG
NcoI 2399 CCATGG C^CATGG
NsiI 4959 ATGCAT ATGCA^T
NspV 2703 TTCGAA TT^CGAA
PaeR7I 2223 CTCGAG C^TCGAG
PpuMI 2403 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 3634 CAGCTG CAG^CTG
SacI 72 GAGCTC GAGCT^C
SacII 25 CCGCGG CCGC^GG
ScaI 5477 AGTACT AGT^ACT
SfoI 21 GGCGCC GGC^GCC
SfuI 2703 TTCGAA TT^CGAA
SnaBI 4056 TACGTA TAC^GTA
XbaI 3660 TCTAGA T^CTAGA
XhoI 2223 CTCGAG C^TCGAG
XmnI 4395 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AspHI 72 GAGCTC G[AT]GC[AT]^C
6734 GTGCAC
AvrII 1984 CCTAGG C^CTAGG
2933 CCTAGG
BbrPI 4761 CACGTG CAC^GTG
6399 CACGTG
BglII 2854 AGATCT A^GATCT
4484 AGATCT
BlnI 1984 CCTAGG C^CTAGG
2933 CCTAGG
BmyI 72 GAGCTC G[AGT]GC[ACT]^C
6734 GTGCAC
BpmI 3737 CTCCAG CTGGAG(16/14)
1904 CTGGAG
BsaBI 323 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6100 GATTTATATC
BsiHKAI 72 GAGCTC G[AT]GC[AT]^C
6734 GTGCAC
BsmI 730 GAATGC GAATGC(1/-1)
5545 GCATTC
Bsp1286I 72 GAGCTC G[AGT]GC[ACT]^C
6734 GTGCAC
BsrFI 44 ACCGGC [GA]^CCGG[CT]
2728 ACCGGC
BstEII 4729 GGTGACC G^GT[ACGT]ACC
5538 GGTAACC
BstPI 4729 GGTGACC G^GT[ACGT]ACC
5538 GGTAACC
CfoI 22 GCGC GCG^C
286 GCGC
Cfr10I 44 ACCGGC [GA]^CCGG[CT]
2728 ACCGGC
DraII 6 GGGGCCT [GA]G^G[ACGT]CC[CT]
2403 GGGACCC
DraIII 3824 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
5868 CACCTTGTG
EaeI 2730 CGGCCA [CT]^GGCC[GA]
6626 TGGCCA
EcoNI 1408 CCTATCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1495 CCTATCTGAGG
EcoO109I 6 GGGGCCT [GA]G^G[ACGT]CC[CT]
2403 GGGACCC
EcoO65I 4729 GGTGACC G^GT[ACGT]ACC
5538 GGTAACC
HaeII 21 GGCGCC [GA]GCGC^[CT]
285 AGCGCC
HgaI 55 GCGTC GACGC(5/10)
2484 GCGTC
HhaI 22 GCGC GCG^C
286 GCGC
HinP1I 22 GCGC G^CGC
286 GCGC
HindIII 300 AAGCTT A^AGCTT
4473 AAGCTT
MamI 323 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6100 GATTTATATC
MspA1I 25 CCGCGG C[AC]G^C[GT]G
3634 CAGCTG
NdeI 3071 CATATG CA^TATG
4437 CATATG
PflMI 645 CCAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
790 CCATCCTTTGG
PmaCI 4761 CACGTG CAC^GTG
6399 CACGTG
PmlI 4761 CACGTG CAC^GTG
6399 CACGTG
PstI 6053 CTGCAG CTGCA^G
6691 CTGCAG
SapI 6392 GAAGAGC GCTCTTC(1/4)
2699 GCTCTTC
SphI 4957 GCATGC GCATG^C
6586 GCATGC
Van91I 645 CCAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
790 CCATCCTTTGG
XcmI 1300 CCATGAAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5550 CCATTGAAAAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
AseI AsnI AvaII BbvI BciVI
BclI BfaI BfrI BsaAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1407I BspHI BspLU11I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DsaI EarI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PshAI
PshBI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII