Gene/Protein Characteristic Table for KIAA0167
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Product ID ORK00439
Accession No D79989
Description ArfGAP with GTPase domain, ankyrin repeat and PH domain 2, transcript variant 2
Clone name ha01026
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (3950 bp)
Predicted protein sequence (844 aa)
Flexi ORF Clone FXC00439
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0167 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 3950 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 1351 bp
Genome contig ID gi89161190r_56305251
PolyA signal sequence
(ATTAAA,-21)
+----*----+----*----+----*----+----
CTTCTTCAACAGCAATTAAAGAGGAAGTGATTTTG
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
TCTAGGGGGTGAGCTGCTGGTCTGTTCTTGGAGGCAGGTCAGGGCATGGC

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 12 r 56405251 56422209 18 100.0 Perfect prediction
Features of the protein sequence
Description

Length: 844 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
AAL04171 0 100.0 centaurin gamma...
Homo sapiens
XP_001116290 0 98.6 centaurin, gamm...
Macaca mulatta
XP_001917890 0 96.8 centaurin, gamm...
Equus caballus
XP_576238 0 95.8 similar to Cent...
Rattus norvegicus
EAW97048 6.2e-170 97.7 centaurin, gamm...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB029022 7.3e-42 55.8 KIAA1099
AB075855 3.4e-36 48.5 KIAA1975
AB051503 4.4e-10 38.9 KIAA1716
D26069 2.3e-09 38.7 KIAA0041
AB033075 4.2e-09 29.8 KIAA1249
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR001806 79 100 PR00449 Ras GTPase
IPR001806 216 238 PR00449 Ras GTPase
IPR001164 595 614 PR00405 Arf GTPase activating protein
IPR001164 614 631 PR00405 Arf GTPase activating protein
IPR001164 635 656 PR00405 Arf GTPase activating protein
IPR002110 743 755 PR01415 Ankyrin
IPR002110 755 767 PR01415 Ankyrin
HMMPfam IPR013684 80 190 PF08477 Miro-like
IPR001849 349 562 PF00169 Pleckstrin-like
IPR001164 583 703 PF01412 Arf GTPase activating protein
IPR002110 742 774 PF00023 Ankyrin
IPR002110 775 800 PF00023 Ankyrin
HMMSmart IPR003577 76 244 SM00173 Ras small GTPase
IPR003579 79 241 SM00175 Ras small GTPase
IPR001849 349 564 SM00233 Pleckstrin-like
IPR001164 583 703 SM00105 Arf GTPase activating protein
ProfileScan IPR001849 348 562 PS50003 Pleckstrin-like
IPR001164 583 703 PS50115 Arf GTPase activating protein
IPR002110 742 774 PS50088 Ankyrin
IPR002110 742 799 PS50297 Ankyrin
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 12
Experimental conditions
Panel name Genebridge 4
Primer_f AAACTGGGTGATAGGGCGGAC
Primer_r AAGATTCACACCACTGCCTCC
PCR product length 215 bp
PCR conditions 95 °C15 sec66 °C60 sec30 cycles
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