A Database of Human Unidentified Gene-Encoded Large Proteins Analyzed
by Kazusa Human cDNA Project
Thank you for your interest in our HUGE Protein Database
The HUGE protein database has been created to publicize the fruits of
our Human cDNA project at the Kazusa DNA Research Institute. In this
project, we plan to sequence and analyze long (>4 kb) human cDNAs and to
establish methods by using the sequence data how to predict the primary
structure of proteins of various biological activities. Currently, we focus
on the analysis of cDNA clones encoding particularly large
proteins (>50 kDa). The basic concept underlying our project and the
strategies employed have been described elsewhere (Ohara et al.,
1997). Our HUGE protein database contains various types of
information derived from the predicted primary structure data of newly
identified human proteins.
The HUGE protein database are expected to cover various sets of large human
proteins of hitherto unidentified functions. They are likely to be involved
in cellular structure/motility (such as cytoskeleton, membrane skeleton,
and motor proteins), gene expression and nucleic acid metabolism, cell
signaling/communication (such as cellular adhesion, signal transduction,
channels, and receptors), and so on.
- Kikuno, R., Nagase, T., Nakayama, M. et al.,
HUGE: a database for human KIAA proteins, a 2004 update integrating HUGEppi and ROUGE. Nucleic Acids Res. 32, D502-504, (2004).
- Ohara, O., Nagase, T., Ishikawa, K.-I. et al., Construction and
characterization of human brain cDNA libraries suitable for analysis of
cDNA clones encoding relatively large proteins. DNA Res., 4,
We thank Takatsugu Hirokawa, Seah Boon-Chieng, and Shigeki Mitaku for
allowing us to use the SOSUI program for the prediction of transmembrane
Database for Clone Resources
KOP (Kazusa ORFeome Project) clones
Flexi ORF clones and Flexi Halotag ORF clones which include the defined open reading
frame are available.
Please visit KOP.
OC (ORFeome Collaboration) clones
As a member of OC,
we started distributing the human ORF clones
from the ORFeome collection.
Kazusa Mouse cDNA project
Rouge (ROdent Unidentified Gene-Encoded large proteins)
Mouse homologues of KIAA and FLJ cDNA clones. Please visit Rouge.
New site-specific recombination systems
VCre/VloxP and SCre/SloxP system
Please visit SSR.