Gene/Protein Characteristic Table for KIAA1516
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Product ID ORK01171
Accession No AB040949
Description phospholipase C, epsilon 1, transcript variant 1
Clone name fh10631s2
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (7989 bp)
Predicted protein sequence (2304 aa)
Flexi ORF Clone FXC01171
Source Human fetal brain
Rouge ID mKIAA1516 by Kazusa Mouse cDNA Project
Note We replaced fh10631, former representative clones for KIAA1516 with fh10631s2. (2003/8/28)
Features of the cloned cDNA sequence
Description

Length: 7989 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 446 bp
Genome contig ID gi89161187f_95643800
PolyA signal sequence
(AATAAA,-25)
+----*----+----*----+----*----+----
GCTGTATTCCAATAAAACTTTATTTATGGACACTG
Flanking genome sequence
(434338 - 434387)
----+----*----+----*----+----*----+----*----+----*
AAATTGGAATTTCATATATTTTTCACATGTCACAAATACTCTTGTTTTGA

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 10 f 95743736 96078136 33 100.0 Perfect prediction
Features of the protein sequence
Description

Length: 2304 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
AAI51855 0 100.0 Phospholipase C...
Homo sapiens
Q9P212 0 100.0 1-phosphatidyli...
Homo sapiens
CAH70739 0 100.0 phospholipase C...
Homo sapiens
AAG17145 0 99.9 phosphoinositid...
Homo sapiens
XP_001149565 0 99.3 pancreas-enrich...
Pan troglodytes
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB028992 9.4e-25 33.6 KIAA1069
AB011153 5.3e-24 27.6 KIAA0581
AB007919 6.7e-23 32.6 KIAA0450
AB075844 1.1e-22 30.5 KIAA1964
AB029015 6.2e-22 27.9 KIAA1092
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
BlastProDom IPR013841 1405 1569 PD001202 Phosphatidylinositol-specific phospholipase C
IPR013841 1752 1844 PD001202 Phosphatidylinositol-specific phospholipase C
FPrintScan IPR001192 1399 1417 PR00390 Phosphoinositide-specific phospholipase C
IPR001192 1425 1445 PR00390 Phosphoinositide-specific phospholipase C
IPR001192 1526 1543 PR00390 Phosphoinositide-specific phospholipase C
IPR001192 1786 1807 PR00390 Phosphoinositide-specific phospholipase C
IPR001192 1807 1825 PR00390 Phosphoinositide-specific phospholipase C
IPR001192 1956 1966 PR00390 Phosphoinositide-specific phospholipase C
HMMPfam IPR001895 553 727 PF00617 Guanine-nucleotide dissociation stimulator CDC25
IPR015359 1330 1381 PF09279 EF-hand-like
IPR000909 1395 1543 PF00388 Phosphatidylinositol-specific phospholipase C
IPR001711 1756 1848 PF00387 Phosphatidylinositol-specific phospholipase C
IPR000008 1873 1947 PF00168 C2 calcium-dependent membrane targeting
IPR000159 2137 2240 PF00788 Ras-association
HMMSmart IPR001895 530 833 SM00147 Guanine-nucleotide dissociation stimulator CDC25
IPR000909 1394 1542 SM00148 Phosphatidylinositol-specific phospholipase C
IPR001711 1731 1848 SM00149 Phosphatidylinositol-specific phospholipase C
IPR000008 1872 1970 SM00239 C2 calcium-dependent membrane targeting
IPR000159 2137 2240 SM00314 Ras-association
ProfileScan IPR001895 533 792 PS50009 Guanine-nucleotide dissociation stimulator CDC25
IPR000909 1394 1542 PS50007 Phosphatidylinositol-specific phospholipase C
IPR001711 1757 1847 PS50008 Phosphatidylinositol-specific phospholipase C
IPR000008 1862 1946 PS50004 C2 calcium-dependent membrane targeting
IPR000159 2137 2240 PS50200 Ras-association
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f CAAGAAGCTCACCAAGTCAAC
Primer_r CTGTCGGTAATCCATTGTGTC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 10
Experimental conditions
Panel name unigene
Primer_f -
Primer_r -
PCR product length -
PCR conditions -
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