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SpecieshumanProduct IDFHC01171Cloning SiteSgfI-PmeISymbolPLCE1
Alias : NPHS3, PLCE, PPLCDescriptionphospholipase C, epsilon 1, transcript variant 1
Characterization of the cloned ORF
N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand
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Length: 6906 bp
Restriction map A
Restriction map B
Genomic Structure
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Length: 2302 aa
Result of homology search against CCDS protein database (
FASTA output,
Multiple alignment)
Entry Exp ID% symbol CCDS41552.1 0 100.0 PLCE1 CCDS53555.1 0 96.5 PLCE1 CCDS54447.1 7.2e-29 36.8 PLCB4 CCDS13105.1 2.4e-28 36.9 PLCB4 CCDS13104.1 2.4e-28 36.9 PLCB4
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
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Result of InterProScan
Search method interpro_ID From To Entry Definition FPrintScan IPR001192 1397 1415 PR00390 Phosphoinositide phospholipase C IPR001192 1423 1443 PR00390 Phosphoinositide phospholipase C IPR001192 1524 1541 PR00390 Phosphoinositide phospholipase C IPR001192 1784 1805 PR00390 Phosphoinositide phospholipase C IPR001192 1805 1823 PR00390 Phosphoinositide phospholipase C IPR001192 1954 1964 PR00390 Phosphoinositide phospholipase C HMMPfam IPR001895 532 715 PF00617 Guanine-nucleotide dissociation stimulator CDC25 IPR015359 1328 1384 PF09279 Phospholipase C IPR000909 1395 1541 PF00388 Phospholipase C IPR001711 1752 1845 PF00387 Phospholipase C IPR000008 1873 1945 PF00168 C2 calcium-dependent membrane targeting IPR000159 2136 2238 PF00788 Ras-association HMMSmart IPR001895 528 831 SM00147 Guanine-nucleotide dissociation stimulator CDC25 IPR000909 1392 1540 SM00148 Phospholipase C IPR001711 1729 1846 SM00149 Phospholipase C IPR000008 1870 1968 SM00239 C2 calcium-dependent membrane targeting IPR000159 2135 2238 SM00314 Ras-association ProfileScan IPR001895 531 790 PS50009 Guanine-nucleotide dissociation stimulator CDC25 IPR000909 1392 1540 PS50007 Phospholipase C IPR001711 1755 1845 PS50008 Phospholipase C IPR018029 1860 1944 PS50004 C2 membrane targeting protein IPR000159 2135 2238 PS50200 Ras-association
Result of homology search against disease genes in OMIM database(FASTA output,
Multiple alignment)
H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_057425 2302 608414,610725 100.0 100.0 NP_001159451 1994 608414,610725 96.5 86.4 XP_006717952 2300 608414,610725 98.7 100.0 XP_006717948 2316 608414,610725 99.4 100.0 XP_011538151 2316 608414,610725 99.4 100.0 Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.comCloned ORF Nucleotide sequence for pFN21AA1516 Download>KIAA1516 6906 bp ATGACTTCTGAAGAAATGACAGCTTCTGTTCTCATACCTGTGACTCAGAGAAAAGTGGTT TCTGCCCAGTCGGCTGCAGATGAAAGTAGTGAAAAGGTCTCAGACATCAATATTTCAAAA GCACATACTGTCAGACGAAGTGGGGAGACTTCTCATACCATCTCACAACTGAACAAACTT AAAGAAGAACCTTCTGGAAGCAACTTGCCAAAGATTCTCTCAATAGCGAGGGAGAAAATA GTGAGTGATGAGAACAGTAATGAAAAATGTTGGGAGAAAATCATGCCAGATTCTGCGAAA AACCTTAACATTAACTGCAACAACATATTGAGAAACCATCAGCATGGCCTTCCTCAGAGA CAATTTTATGAAATGTACAACTCTGTTGCTGAGGAAGACTTGTGTTTAGAAACTGGAATT CCTTCTCCACTGGAAAGAAAGGTGTTCCCTGGAATTCAACTGGAACTAGACAGACCTTCC ATGGGCATTAGTCCTTTAGGAAATCAGTCAGTGATCATAGAGACAGGCAGAGCACACCCT GACAGCAGAAGGGCAGTATTTCATTTTCATTATGAAGTTGACAGAAGAATGTCAGACACT TTCTGTACCCTATCAGAAAACTTAATTTTAGACGATTGTGGAAATTGTGTACCACTACCT GGGGGTGAGGAGAAGCAAAAGAAAAACTATGTGGCATATACCTGTAAACTGATGGAATTG GCCAAAAATTGTGATAATAAGAATGAGCAGCTGCAGTGTGATCATTGTGACACCTTGAAT GATAAATACTTTTGCTTTGAAGGCTCTTGTGAGAAGGTTGACATGGTATATTCAGGTGAT AGCTTTTGTAGGAAAGACTTTACTGACAGTCAAGCTGCCAAGACCTTTTTGAGCCATTTT GAGGACTTCCCTGATAATTGTGATGATGTAGAAGAAGACGCTTTTAAAAGCAAAAAGGAG CGATCCACTTTGTTAGTCAGGAGATTCTGTAAAAATGACAGAGAAGTTAAGAAATCTGTG TATACTGGAACAAGAGCAATTGTGAGAACTCTGCCTTCTGGCCACATTGGGCTGACTGCA TGGAGTTACATAGATCAGAAGAGAAATGGTCCCTTACTGCCTTGTGGGAGAGTAATGGAA CCCCCGTCAACAGTGGAGATAAGGCAAGATGGGAGCCAACGTCTGTCAGAAGCCCAGTGG TATCCTATCTACAATGCAGTGAGAAGAGAAGAAACAGAAAATACAGTTGGATCTCTACTC CATTTCCTCACCAAGCTCCCAGCCTCCGAGACAGCCCATGGAAGGATAAGCGTTGGTCCA TGCTTAAAGCAATGTGTCCGAGACACTGTATGTGAGTATCGCGCCACCCTCCAAAGGACT TCAATATCGCAGTACATCACCGGTTCTCTCCTAGAAGCAACCACGTCTTTGGGAGCAAGA AGTGGCCTTCTCAGTACTTTTGGAGGATCCACTGGACGAATGATGCTGAAAGAACGCCAG CCAGGCCCCTCTGTGGCCAATTCCAATGCCCTCCCTTCAAGTTCAGCTGGGATCAGCAAG GAGCTGATCGATCTGCAGCCTCTCATCCAGTTCCCAGAGGAAGTCGCCAGCATCCTGATG GAGCAAGAGCAGACTATTTACCGCAGGGTCTTGCCAGTCGACTACCTTTGCTTCTTAACA CGGGACTTGGGCACTCCTGAATGCCAGAGCTCCTTGCCCTGCCTCAAAGCATCCATCTCA GCGTCGATTCTTACCACTCAGAATGGAGAGCACAATGCCCTTGAAGATCTGGTGATGAGG TTTAATGAGGTGAGCTCCTGGGTGACATGGCTGATCCTCACGGCAGGCTCCATGGAGGAG AAGCGAGAAGTCTTTTCATATTTGGTGCATGTGGCCAAATGCTGCTGGAACATGGGCAAC TACAACGCTGTCATGGAGTTCTTGGCTGGCCTCAGGTCAAGAAAAGTTTTAAAAATGTGG CAGTTCATGGACCAGTCTGATATTGAGACCATGAGGAGCCTGAAGGATGCTATGGCCCAG CATGAGTCCTCTTGTGAGTACAGAAAGGTGGTGACACGTGCCCTGCACATCCCTGGCTGT AAGGTGGTTCCATTCTGTGGGGTGTTTCTGAAGGAGCTCTGTGAAGTGCTTGACGGCGCC TCCGGTCTCATGAAGCTTTGCCCGCGGTACAATTCCCAAGAAGAAACTTTAGAGTTTGTA GCAGATTACAGTGGACAAGATAATTTCTTACAACGAGTGGGACAAAATGGCTTAAAGAAT TCGGAGAAGGAGTCCACTGTCAACAGCATCTTTCAGGTCATCCGGAGCTGCAATCGAAGT CTGGAGACAGACGAGGAGGACAGCCCCAGTGAAGGAAACAGCTCCAGGAAAAGCTCCTTG AAGGATAAAAGCCGATGGCAGTTTATAATTGGAGATTTGTTGGATTCAGACAATGACATC TTTGAGCAATCCAAAGAATACGACTCTCATGGTTCAGAGGACTCACAGAAGGCCTTCGAC CATGGGACGGAGCTCATCCCTTGGTACGTGCTGTCCATCCAAGCCGATGTGCACCAGTTC CTGCTGCAGGGGGCCACGGTCATCCACTACGACCAGGACACACACCTCTCTGCCCGCTGC TTCCTCCAGCTTCAGCCCGACAATAGCACCTTGACCTGGGTAAAGCCCACAACTGCCTCC CCAGCCAGCAGTAAAGCAAAACTTGGTGTACTTAATAACACAGCTGAGCCTGGAAAATTC CCACTACTGGGTAATGCTGGATTAAGTAGCCTGACGGAAGGGGTCTTGGATCTTTTTGCA GTGAAGGCTGTATACATGGGCCACCCTGGCATTGATATACACACTGTGTGTGTTCAGAAC AAACTGGGTAGCATGTTCCTGTCAGAGACTGGTGTGACATTGCTCTATGGGCTTCAGACC ACAGACAACAGATTATTGCACTTCGTGGCACCAAAGCACACAGCTAAAATGCTCTTCAGC GGATTATTGGAACTCACTAGAGCTGTGAGAAAGATGAGGAAATTCCCTGACCAAAGACAG CAGTGGCTGCGGAAACAGTACGTCAGCCTTTATCAGGAGGATGGACGGTATGAAGGCCCA ACTTTGGCTCACGCTGTGGAGTTGTTTGGTGGCAGACGGTGGAGTGCTCGAAACCCCAGC CCCGGAACATCAGCAAAGAATGCTGAGAAGCCCAATATGCAGAGAAACAATACCCTGGGC ATAAGCACTACCAAGAAAAAGAAGAAAATCCTCATGAGGGGTGAGAGTGGAGAGGTAACT GACGATGAGATGGCAACCCGAAAGGCCAAGATGCACAAAGAGTGTCGAAGCCGGAGTGGT TCTGATCCTCAAGACATTAATGAACAAGAAGAATCAGAGGTGAATGCCATCGCTAACCCT CCAAACCCCCTCCCTTCCAGAAGAGCCCACTCTTTGACCACAGCTGGGTCCCCCAACTTG GCTGCCGGGACGTCATCTCCCATCAGGCCAGTGTCCTCCCCTGTGCTGTCTTCTTCAAAC AAGAGCCCATCCAGTGCTTGGAGCAGTAGTAGCTGGCACGGGCGGATCAAAGGCGGCATG AAGGGATTTCAGAGCTTCATGGTTTCAGATAGCAACATGAGTTTTGTTGAATTTGTTGAG CTGTTCAAATCATTCAGTGTCAGGAGCCGCAAGGACCTGAAGGATCTGTTTGATGTCTAT GCAGTGCCCTGCAACCGATCTGGCTCCGAGTCAGCCCCACTCTACACCAACCTGACAATT GATGAAAACACCAGCGATCTTCAGCCTGACCTAGATCTGTTGACCAGAAATGTCTCGGAT TTGGGGTTGTTCATTAAGAGTAAACAGCAGCTATCGGACAACCAGAGGCAGATATCTGAT GCCATTGCTGCTGCAAGCATTGTGACAAATGGCACTGGGATTGAGAGCACATCTCTGGGC ATTTTTGGGGTGGGCATACTTCAGCTCAACGATTTCCTCGTGAATTGCCAAGGAGAACAC TGCACTTATGATGAAATCCTCAGCATCATCCAGAAGTTCGAGCCTAGCATCAGTATGTGT CATCAGGGACTAATGTCATTTGAAGGGTTTGCCAGGTTTCTGATGGATAAAGAAAATTTT GCCTCAAAAAATGATGAGTCACAGGAGAACATTAAAGAACTGCAGCTACCCCTCTCATAC TATTACATCGAATCTTCGCACAATACCTACCTCACGGGCCATCAGCTCAAAGGAGAATCC TCGGTAGAACTCTACAGCCAGGTCCTTTTGCAAGGCTGTCGAAGTGTAGAATTGGACTGC TGGGACGGAGACGATGGGATGCCCATCATTTATCATGGACATACGCTGACAACCAAGATC CCCTTCAAGGAAGTGGTTGAAGCCATTGATCGCAGTGCCTTCATCAACTCTGACCTGCCA ATCATCATATCGATTGAGAACCACTGTTCATTGCCTCAGCAACGAAAAATGGCAGAAATT TTCAAGACTGTGTTTGGAGAAAAGCTGGTGACTAAATTCTTATTTGAGACTGATTTCTCA GATGATCCAATGCTTCCTTCACCTGACCAACTCAGAAAGAAAGTTCTTCTTAAAAACAAG AAGCTAAAAGCCCATCAGACGCCAGTGGATATCTTAAAGCAAAAGGCTCATCAGTTAGCA TCTATGCAAGTGCAGGCTTATAATGGTGGGAATGCCAACCCCCGACCTGCCAATAATGAG GAAGAGGAAGATGAGGAGGACGAATATGATTATGACTATGAATCCCTTTCTGATGACAAC ATTCTGGAAGACAGACCTGAAAATAAATCATGTAATGACAAGCTTCAGTTTGAATATAAT GAAGAAATCCCAAAGAGGATAAAGAAAGCAGATAACTCTGCTTGCAACAAAGGAAAGGTT TATGATATGGAACTGGGAGAAGAATTTTATCTTGATCAGAATAAAAAGGAAAGCAGACAG ATTGCACCAGAGCTTTCTGACCTTGTAATCTATTGTCAAGCAGTAAAATTTCCAGGACTG TCAACTCTAAATGCATCTGGCTCTAGCAGAGGAAAAGAAAGGAAAAGCAGGAAGTCCATT TTTGGCAACAATCCGGGCAGAATGAGCCCAGGGGAGACAGCATCATTTAACAAAACATCT GGAAAAAGTTCCTGTGAAGGCATTCGACAGACCTGGGAGGAATCTTCTTCCCCTCTCAAC CCAACCACGTCCCTCAGTGCTATCATTAGAACTCCCAAATGTTATCATATCTCGTCGCTG AATGAAAATGCCGCCAAACGTCTGTGTCGCAGGTATTCTCAGAAACTGACCCAGCACACC GCCTGTCAGCTGCTGAGAACTTACCCTGCTGCCACCCGCATCGACTCTTCCAACCCGAAC CCCCTCATGTTCTGGCTCCATGGGATACAGCTTGTGGCACTCAACTACCAGACTGATGAT CTCCCTTTACATTTAAATGCTGCAATGTTTGAGGCAAATGGTGGTTGTGGTTATGTATTG AAACCTCCAGTTCTGTGGGACAAGAACTGCCCCATGTATCAGAAGTTTTCTCCACTAGAA AGAGATCTGGACAGCATGGATCCTGCAGTCTATTCTTTAACTATTGTCTCTGGTCAGAAT GTGTGCCCCAGTAATAGCATGGGAAGCCCGTGCATTGAAGTCGACGTCCTGGGCATGCCT CTGGACAGCTGCCATTTCCGCACAAAGCCCATCCATCGAAACACCCTGAACCCCATGTGG AACGAGCAGTTTCTGTTCCGCGTTCACTTCGAAGATCTTGTATTTCTTCGTTTTGCAGTT GTGGAAAACAATAGTTCAGCGGTAACTGCTCAGAGAATCATTCCACTGAAAGCTTTAAAA CGAGGATATCGACATCTTCAGCTGCGAAACCTTCACAATGAAGTCTTGGAGATTTCTAGT TTATTCATTAACAGCAGAAGGATGGAAGAAAATTCCTCTGGCAATACCATGTCAGCCTCT TCGATGTTTAATACAGAAGAAAGAAAATGTTTGCAGACTCACAGAGTCACGGTGCATGGG GTCCCAGGGCCAGAGCCCTTTACCGTTTTCACTATTAATGGAGGCACCAAGGCAAAGCAG CTTCTGCAGCAAATTCTGACAAATGAACAAGACATCAAACCTGTTACCACAGACTATTTT TTGATGGAAGAAAAATATTTTATATCTAAAGAAAAGAATGAATGTAGGAAACAACCATTC CAGAGAGCCATTGGTCCAGAAGAGGAGATCATGCAAATTTTAAGCAGCTGGTTTCCAGAA GAGGGATACATGGGCAGGATTGTCTTAAAAACCCAGCAGGAAAACCTAGAAGAGAAAAAC ATTGTTCAAGATGACAAAGAGGTGATCTTGAGCTCAGAGGAGGAGAGTTTCTTTGTCCAA GTGCATGATGTTTCTCCAGAGCAACCTCGAACAGTCATCAAAGCACCCCGCGTCAGCACT GCACAGGATGTCATTCAGCAGACCTTATGCAAAGCCAAATATTCCTACAGCATCCTGAGC AACCCCAATCCAAGCGACTATGTGCTTTTGGAAGAGGTGGTGAAAGACACTACCAACAAG AAGACTACCACACCAAAGTCCTCTCAGCGGGTCCTTCTGGATCAGGAGTGTGTGTTTCAA GCCCAAAGCAAGTGGAAAGGTGCAGGAAAATTCATCCTTAAGCTAAAGGAGCAGGTGCAG GCATCTCGAGAAGATAAAAAGAAAGGCATTTCTTTCGCAAGTGAACTCAAGAAGCTCACC AAGTCAACTAAACAGCCCCGAGGACTTACATCACCTTCTCAGCTCTTGACCTCAGAAAGT ATCCAAACCAAGGAGGAGAAACCTGTGGGTGGCTTGTCCTCCAGTGACACAATGGATTAC CGACAG
Cloned ORF protein sequence for pFN21AA1516 Download>KIAA1516 2302 aa MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKL KEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQR QFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHP DSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMEL AKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHF EDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTA WSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLL HFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGAR SGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILM EQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMR FNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMW QFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGA SGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRS LETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFD HGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTAS PASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQN KLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQ QWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLG ISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANP PNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGM KGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTI DENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLG IFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENF ASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDC WDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEI FKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLA SMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDDNILEDRPENKSCNDKLQFEYN EEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGL STLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLN PTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPN PLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLE RDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMW NEQFLFRVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISS LFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQ LLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPE EGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVST AQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQ AQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSES IQTKEEKPVGGLSSSDTMDYRQ
Nucleotide Sequence (with vector) for pFN21AA1516 Download>pFN21AA1516 11611 bp TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA CTTTCAGAGCGATAACGCGATCGCCATGACTTCTGAAGAAATGACAGCTTCTGTTCTCAT ACCTGTGACTCAGAGAAAAGTGGTTTCTGCCCAGTCGGCTGCAGATGAAAGTAGTGAAAA GGTCTCAGACATCAATATTTCAAAAGCACATACTGTCAGACGAAGTGGGGAGACTTCTCA TACCATCTCACAACTGAACAAACTTAAAGAAGAACCTTCTGGAAGCAACTTGCCAAAGAT TCTCTCAATAGCGAGGGAGAAAATAGTGAGTGATGAGAACAGTAATGAAAAATGTTGGGA GAAAATCATGCCAGATTCTGCGAAAAACCTTAACATTAACTGCAACAACATATTGAGAAA CCATCAGCATGGCCTTCCTCAGAGACAATTTTATGAAATGTACAACTCTGTTGCTGAGGA AGACTTGTGTTTAGAAACTGGAATTCCTTCTCCACTGGAAAGAAAGGTGTTCCCTGGAAT TCAACTGGAACTAGACAGACCTTCCATGGGCATTAGTCCTTTAGGAAATCAGTCAGTGAT CATAGAGACAGGCAGAGCACACCCTGACAGCAGAAGGGCAGTATTTCATTTTCATTATGA AGTTGACAGAAGAATGTCAGACACTTTCTGTACCCTATCAGAAAACTTAATTTTAGACGA TTGTGGAAATTGTGTACCACTACCTGGGGGTGAGGAGAAGCAAAAGAAAAACTATGTGGC ATATACCTGTAAACTGATGGAATTGGCCAAAAATTGTGATAATAAGAATGAGCAGCTGCA GTGTGATCATTGTGACACCTTGAATGATAAATACTTTTGCTTTGAAGGCTCTTGTGAGAA GGTTGACATGGTATATTCAGGTGATAGCTTTTGTAGGAAAGACTTTACTGACAGTCAAGC TGCCAAGACCTTTTTGAGCCATTTTGAGGACTTCCCTGATAATTGTGATGATGTAGAAGA AGACGCTTTTAAAAGCAAAAAGGAGCGATCCACTTTGTTAGTCAGGAGATTCTGTAAAAA TGACAGAGAAGTTAAGAAATCTGTGTATACTGGAACAAGAGCAATTGTGAGAACTCTGCC TTCTGGCCACATTGGGCTGACTGCATGGAGTTACATAGATCAGAAGAGAAATGGTCCCTT ACTGCCTTGTGGGAGAGTAATGGAACCCCCGTCAACAGTGGAGATAAGGCAAGATGGGAG CCAACGTCTGTCAGAAGCCCAGTGGTATCCTATCTACAATGCAGTGAGAAGAGAAGAAAC AGAAAATACAGTTGGATCTCTACTCCATTTCCTCACCAAGCTCCCAGCCTCCGAGACAGC CCATGGAAGGATAAGCGTTGGTCCATGCTTAAAGCAATGTGTCCGAGACACTGTATGTGA GTATCGCGCCACCCTCCAAAGGACTTCAATATCGCAGTACATCACCGGTTCTCTCCTAGA AGCAACCACGTCTTTGGGAGCAAGAAGTGGCCTTCTCAGTACTTTTGGAGGATCCACTGG ACGAATGATGCTGAAAGAACGCCAGCCAGGCCCCTCTGTGGCCAATTCCAATGCCCTCCC TTCAAGTTCAGCTGGGATCAGCAAGGAGCTGATCGATCTGCAGCCTCTCATCCAGTTCCC AGAGGAAGTCGCCAGCATCCTGATGGAGCAAGAGCAGACTATTTACCGCAGGGTCTTGCC AGTCGACTACCTTTGCTTCTTAACACGGGACTTGGGCACTCCTGAATGCCAGAGCTCCTT GCCCTGCCTCAAAGCATCCATCTCAGCGTCGATTCTTACCACTCAGAATGGAGAGCACAA TGCCCTTGAAGATCTGGTGATGAGGTTTAATGAGGTGAGCTCCTGGGTGACATGGCTGAT CCTCACGGCAGGCTCCATGGAGGAGAAGCGAGAAGTCTTTTCATATTTGGTGCATGTGGC CAAATGCTGCTGGAACATGGGCAACTACAACGCTGTCATGGAGTTCTTGGCTGGCCTCAG GTCAAGAAAAGTTTTAAAAATGTGGCAGTTCATGGACCAGTCTGATATTGAGACCATGAG GAGCCTGAAGGATGCTATGGCCCAGCATGAGTCCTCTTGTGAGTACAGAAAGGTGGTGAC ACGTGCCCTGCACATCCCTGGCTGTAAGGTGGTTCCATTCTGTGGGGTGTTTCTGAAGGA GCTCTGTGAAGTGCTTGACGGCGCCTCCGGTCTCATGAAGCTTTGCCCGCGGTACAATTC CCAAGAAGAAACTTTAGAGTTTGTAGCAGATTACAGTGGACAAGATAATTTCTTACAACG AGTGGGACAAAATGGCTTAAAGAATTCGGAGAAGGAGTCCACTGTCAACAGCATCTTTCA GGTCATCCGGAGCTGCAATCGAAGTCTGGAGACAGACGAGGAGGACAGCCCCAGTGAAGG AAACAGCTCCAGGAAAAGCTCCTTGAAGGATAAAAGCCGATGGCAGTTTATAATTGGAGA TTTGTTGGATTCAGACAATGACATCTTTGAGCAATCCAAAGAATACGACTCTCATGGTTC AGAGGACTCACAGAAGGCCTTCGACCATGGGACGGAGCTCATCCCTTGGTACGTGCTGTC CATCCAAGCCGATGTGCACCAGTTCCTGCTGCAGGGGGCCACGGTCATCCACTACGACCA GGACACACACCTCTCTGCCCGCTGCTTCCTCCAGCTTCAGCCCGACAATAGCACCTTGAC CTGGGTAAAGCCCACAACTGCCTCCCCAGCCAGCAGTAAAGCAAAACTTGGTGTACTTAA TAACACAGCTGAGCCTGGAAAATTCCCACTACTGGGTAATGCTGGATTAAGTAGCCTGAC GGAAGGGGTCTTGGATCTTTTTGCAGTGAAGGCTGTATACATGGGCCACCCTGGCATTGA TATACACACTGTGTGTGTTCAGAACAAACTGGGTAGCATGTTCCTGTCAGAGACTGGTGT GACATTGCTCTATGGGCTTCAGACCACAGACAACAGATTATTGCACTTCGTGGCACCAAA GCACACAGCTAAAATGCTCTTCAGCGGATTATTGGAACTCACTAGAGCTGTGAGAAAGAT GAGGAAATTCCCTGACCAAAGACAGCAGTGGCTGCGGAAACAGTACGTCAGCCTTTATCA GGAGGATGGACGGTATGAAGGCCCAACTTTGGCTCACGCTGTGGAGTTGTTTGGTGGCAG ACGGTGGAGTGCTCGAAACCCCAGCCCCGGAACATCAGCAAAGAATGCTGAGAAGCCCAA TATGCAGAGAAACAATACCCTGGGCATAAGCACTACCAAGAAAAAGAAGAAAATCCTCAT GAGGGGTGAGAGTGGAGAGGTAACTGACGATGAGATGGCAACCCGAAAGGCCAAGATGCA CAAAGAGTGTCGAAGCCGGAGTGGTTCTGATCCTCAAGACATTAATGAACAAGAAGAATC AGAGGTGAATGCCATCGCTAACCCTCCAAACCCCCTCCCTTCCAGAAGAGCCCACTCTTT GACCACAGCTGGGTCCCCCAACTTGGCTGCCGGGACGTCATCTCCCATCAGGCCAGTGTC CTCCCCTGTGCTGTCTTCTTCAAACAAGAGCCCATCCAGTGCTTGGAGCAGTAGTAGCTG GCACGGGCGGATCAAAGGCGGCATGAAGGGATTTCAGAGCTTCATGGTTTCAGATAGCAA CATGAGTTTTGTTGAATTTGTTGAGCTGTTCAAATCATTCAGTGTCAGGAGCCGCAAGGA CCTGAAGGATCTGTTTGATGTCTATGCAGTGCCCTGCAACCGATCTGGCTCCGAGTCAGC CCCACTCTACACCAACCTGACAATTGATGAAAACACCAGCGATCTTCAGCCTGACCTAGA TCTGTTGACCAGAAATGTCTCGGATTTGGGGTTGTTCATTAAGAGTAAACAGCAGCTATC GGACAACCAGAGGCAGATATCTGATGCCATTGCTGCTGCAAGCATTGTGACAAATGGCAC TGGGATTGAGAGCACATCTCTGGGCATTTTTGGGGTGGGCATACTTCAGCTCAACGATTT CCTCGTGAATTGCCAAGGAGAACACTGCACTTATGATGAAATCCTCAGCATCATCCAGAA GTTCGAGCCTAGCATCAGTATGTGTCATCAGGGACTAATGTCATTTGAAGGGTTTGCCAG GTTTCTGATGGATAAAGAAAATTTTGCCTCAAAAAATGATGAGTCACAGGAGAACATTAA AGAACTGCAGCTACCCCTCTCATACTATTACATCGAATCTTCGCACAATACCTACCTCAC GGGCCATCAGCTCAAAGGAGAATCCTCGGTAGAACTCTACAGCCAGGTCCTTTTGCAAGG CTGTCGAAGTGTAGAATTGGACTGCTGGGACGGAGACGATGGGATGCCCATCATTTATCA TGGACATACGCTGACAACCAAGATCCCCTTCAAGGAAGTGGTTGAAGCCATTGATCGCAG TGCCTTCATCAACTCTGACCTGCCAATCATCATATCGATTGAGAACCACTGTTCATTGCC TCAGCAACGAAAAATGGCAGAAATTTTCAAGACTGTGTTTGGAGAAAAGCTGGTGACTAA ATTCTTATTTGAGACTGATTTCTCAGATGATCCAATGCTTCCTTCACCTGACCAACTCAG AAAGAAAGTTCTTCTTAAAAACAAGAAGCTAAAAGCCCATCAGACGCCAGTGGATATCTT AAAGCAAAAGGCTCATCAGTTAGCATCTATGCAAGTGCAGGCTTATAATGGTGGGAATGC CAACCCCCGACCTGCCAATAATGAGGAAGAGGAAGATGAGGAGGACGAATATGATTATGA CTATGAATCCCTTTCTGATGACAACATTCTGGAAGACAGACCTGAAAATAAATCATGTAA TGACAAGCTTCAGTTTGAATATAATGAAGAAATCCCAAAGAGGATAAAGAAAGCAGATAA CTCTGCTTGCAACAAAGGAAAGGTTTATGATATGGAACTGGGAGAAGAATTTTATCTTGA TCAGAATAAAAAGGAAAGCAGACAGATTGCACCAGAGCTTTCTGACCTTGTAATCTATTG TCAAGCAGTAAAATTTCCAGGACTGTCAACTCTAAATGCATCTGGCTCTAGCAGAGGAAA AGAAAGGAAAAGCAGGAAGTCCATTTTTGGCAACAATCCGGGCAGAATGAGCCCAGGGGA GACAGCATCATTTAACAAAACATCTGGAAAAAGTTCCTGTGAAGGCATTCGACAGACCTG GGAGGAATCTTCTTCCCCTCTCAACCCAACCACGTCCCTCAGTGCTATCATTAGAACTCC CAAATGTTATCATATCTCGTCGCTGAATGAAAATGCCGCCAAACGTCTGTGTCGCAGGTA TTCTCAGAAACTGACCCAGCACACCGCCTGTCAGCTGCTGAGAACTTACCCTGCTGCCAC CCGCATCGACTCTTCCAACCCGAACCCCCTCATGTTCTGGCTCCATGGGATACAGCTTGT GGCACTCAACTACCAGACTGATGATCTCCCTTTACATTTAAATGCTGCAATGTTTGAGGC AAATGGTGGTTGTGGTTATGTATTGAAACCTCCAGTTCTGTGGGACAAGAACTGCCCCAT GTATCAGAAGTTTTCTCCACTAGAAAGAGATCTGGACAGCATGGATCCTGCAGTCTATTC TTTAACTATTGTCTCTGGTCAGAATGTGTGCCCCAGTAATAGCATGGGAAGCCCGTGCAT TGAAGTCGACGTCCTGGGCATGCCTCTGGACAGCTGCCATTTCCGCACAAAGCCCATCCA TCGAAACACCCTGAACCCCATGTGGAACGAGCAGTTTCTGTTCCGCGTTCACTTCGAAGA TCTTGTATTTCTTCGTTTTGCAGTTGTGGAAAACAATAGTTCAGCGGTAACTGCTCAGAG AATCATTCCACTGAAAGCTTTAAAACGAGGATATCGACATCTTCAGCTGCGAAACCTTCA CAATGAAGTCTTGGAGATTTCTAGTTTATTCATTAACAGCAGAAGGATGGAAGAAAATTC CTCTGGCAATACCATGTCAGCCTCTTCGATGTTTAATACAGAAGAAAGAAAATGTTTGCA GACTCACAGAGTCACGGTGCATGGGGTCCCAGGGCCAGAGCCCTTTACCGTTTTCACTAT TAATGGAGGCACCAAGGCAAAGCAGCTTCTGCAGCAAATTCTGACAAATGAACAAGACAT CAAACCTGTTACCACAGACTATTTTTTGATGGAAGAAAAATATTTTATATCTAAAGAAAA GAATGAATGTAGGAAACAACCATTCCAGAGAGCCATTGGTCCAGAAGAGGAGATCATGCA AATTTTAAGCAGCTGGTTTCCAGAAGAGGGATACATGGGCAGGATTGTCTTAAAAACCCA GCAGGAAAACCTAGAAGAGAAAAACATTGTTCAAGATGACAAAGAGGTGATCTTGAGCTC AGAGGAGGAGAGTTTCTTTGTCCAAGTGCATGATGTTTCTCCAGAGCAACCTCGAACAGT CATCAAAGCACCCCGCGTCAGCACTGCACAGGATGTCATTCAGCAGACCTTATGCAAAGC CAAATATTCCTACAGCATCCTGAGCAACCCCAATCCAAGCGACTATGTGCTTTTGGAAGA GGTGGTGAAAGACACTACCAACAAGAAGACTACCACACCAAAGTCCTCTCAGCGGGTCCT TCTGGATCAGGAGTGTGTGTTTCAAGCCCAAAGCAAGTGGAAAGGTGCAGGAAAATTCAT CCTTAAGCTAAAGGAGCAGGTGCAGGCATCTCGAGAAGATAAAAAGAAAGGCATTTCTTT CGCAAGTGAACTCAAGAAGCTCACCAAGTCAACTAAACAGCCCCGAGGACTTACATCACC TTCTCAGCTCTTGACCTCAGAAAGTATCCAAACCAAGGAGGAGAAACCTGTGGGTGGCTT GTCCTCCAGTGACACAATGGATTACCGACAGGTTTAAACGAATTCGGGCTCGGTACCCGG GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC ACGATGCGTCCGGCGTAGAGGATCGAGATCT
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