##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
Acc65I AclI Aor51HI ApaI Asp718I
AspI AviII AvrII BlnI BsiEI
BsiWI BsrBI BstEII BstPI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I FseI FspI HpaI KpnI
MluI NdeI NotI NruI PacI
PflFI PmeI Psp1406I PvuI RsrII
SbfI SexAI SfiI SnaBI SpeI
Sse8387I Tth111I
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccIII 2955 TCCGGA T^CCGGA
AflII 7331 CTTAAG C^TTAAG
AgeI 2033 ACCGGT A^CCGGT
AhdI 207 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3203 GTGCAC G^TGCAC
AocI 2584 CCTCAGG CC^T[ACGT]AGG
ApaLI 3203 GTGCAC G^TGCAC
AscI 61 GGCGCGCC GG^CGCGCC
AspEI 207 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2789 GGCGCC GGCGC^C
BbrPI 2709 CACGTG CAC^GTG
BfrI 7331 CTTAAG C^TTAAG
BlpI 3377 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3377 GCTGAGC GC^T[ACGT]AGC
BsaBI 4972 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2955 TCCGGA T^CCGGA
BsmBI 4962 GAGACG CGTCTC(1/5)
Bsp1407I 1008 TGTACA T^GTACA
BspEI 2955 TCCGGA T^CCGGA
BspLU11I 7676 ACATGT A^CATGT
BsrGI 1008 TGTACA T^GTACA
BssSI 4631 CTCGTG CACGAG(-5/-1)
BstBI 6422 TTCGAA TT^CGAA
Bsu36I 2584 CCTCAGG CC^T[ACGT]AGG
CelII 3377 GCTGAGC GC^T[ACGT]AGC
Eam1105I 207 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2584 CCTCAGG CC^T[ACGT]AGG
EcoT22I 5685 ATGCAT ATGCA^T
HaeII 2789 GGCGCC [GA]GCGC^[CT]
KasI 2789 GGCGCC G^GCGCC
KspI 2816 CCGCGG CCGC^GG
MamI 4972 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2955 TCCGGA T^CCGGA
NaeI 243 GCCGGC GCC^GGC
NarI 2789 GGCGCC GG^CGCC
NgoMIV 243 GCCGGC G^CCGGC
NheI 189 GCTAGC G^CTAGC
NsiI 5685 ATGCAT ATGCA^T
NspV 6422 TTCGAA TT^CGAA
PaeR7I 7359 CTCGAG C^TCGAG
PinAI 2033 ACCGGT A^CCGGT
PmaCI 2709 CACGTG CAC^GTG
PmlI 2709 CACGTG CAC^GTG
PshAI 4722 GACTAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 2816 CCGCGG CCGC^GG
ScaI 2087 AGTACT AGT^ACT
SfoI 2789 GGCGCC GGC^GCC
SfuI 6422 TTCGAA TT^CGAA
SgrAI 242 CGCCGGCG C[GA]^CCGG[CT]G
SphI 6327 GCATGC GCATG^C
SspBI 1008 TGTACA T^GTACA
StuI 3144 AGGCCT AGG^CCT
SwaI 6105 ATTTAAAT ATTT^AAAT
XbaI 422 TCTAGA T^CTAGA
XhoI 7359 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AatII 4123 GACGTC GACGT^C
6317 GACGTC
AflIII 2708 ACACGT A^C[GA][CT]GT
7676 ACATGT
AseI 4010 ATTAAT AT^TAAT
6728 ATTAAT
AsnI 4010 ATTAAT AT^TAAT
6728 ATTAAT
BamHI 2099 GGATCC G^GATCC
6232 GGATCC
BsaAI 2709 CACGTG [CT]AC^GT[GA]
3179 TACGTG
BspDI 2201 ATCGAT AT^CGAT
5083 ATCGAT
BsrFI 243 GCCGGC [GA]^CCGG[CT]
2033 ACCGGT
BssHII 62 GCGCGC G^CGCGC
200 GCGCGC
Bst1107I 1654 GTATAC GTA^TAC
3464 GTATAC
BstZ17I 1654 GTATAC GTA^TAC
3464 GTATAC
Cfr10I 243 GCCGGC [GA]^CCGG[CT]
2033 ACCGGT
ClaI 2201 ATCGAT AT^CGAT
5083 ATCGAT
EcoNI 3214 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3721 CCTTTATCAGG
MfeI 1671 CAATTG C^AATTG
4410 CAATTG
MunI 1671 CAATTG C^AATTG
4410 CAATTG
PshBI 4010 ATTAAT AT^TAAT
6728 ATTAAT
SalI 2291 GTCGAC G^TCGAC
6314 GTCGAC
SapI 4074 GAAGAGC GCTCTTC(1/4)
3625 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcgI BciVI BclI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoO109I EcoRI EcoRII EcoRV
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI