Gene/Protein Characteristic Table for KIAA0581
Order Kazusa clone(s) from : Japan || Other countries
Product ID ORK01979
Accession No AB011153
Description phospholipase C, beta 1 (phosphoinositide-specific), transcript variant 1
Clone name pf10976
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (7092 bp)
Predicted protein sequence (1218 aa)
Flexi ORF Clone FXC01979
Source Human brain (hippocampus)
Rouge ID mKIAA0581 by Kazusa Mouse cDNA Project
Note We replaced hj00610 and hj00610s1, former representative clones for KIAA0581 with pf10976. (2002/5/10,2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 7092 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 3054 bp
Genome contig ID gi51511747f_7960912
PolyA signal sequence
(AATAAA,-24)
+----*----+----*----+----*----+----
CCAAAGGAAAGAATAAAAATTTCTTAACACAACCC
Flanking genome sequence
(852640 - 852689)
----+----*----+----*----+----*----+----*----+----*
AGCGGTTATTTCTTGGGGTCAGAGTCAACCTTCTTTTGACAGGGAAAGTG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 20 f 8060912 8813550 32 99.5 Perfect prediction
Features of the protein sequence
Description

Length: 1218 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q9NQ66 0 100.0 1-phosphatidyli...
Homo sapiens
XP_001116017 0 99.2 similar to phos...
Macaca mulatta
XP_001170216 0 99.7 phosphoinositid...
Pan troglodytes
P10894 0 97.5 1-phosphatidyli...
Bos taurus
1611219A 0 97.4 phospholipase C...
Bos taurus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB040949 3.6e-14 27.7 KIAA1516
AB007919 6.4e-14 30.5 KIAA0450
AB029015 2e-12 31.0 KIAA1092
AB028992 5.4e-11 30.5 KIAA1069
AB075844 7e-11 30.9 KIAA1964
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
BlastProDom IPR013841 329 654 PD001202 Phosphatidylinositol-specific phospholipase C
FPrintScan IPR001192 323 341 PR00390 Phosphoinositide-specific phospholipase C
IPR001192 349 369 PR00390 Phosphoinositide-specific phospholipase C
IPR001192 453 470 PR00390 Phosphoinositide-specific phospholipase C
IPR001192 596 617 PR00390 Phosphoinositide-specific phospholipase C
IPR001192 617 635 PR00390 Phosphoinositide-specific phospholipase C
IPR001192 764 774 PR00390 Phosphoinositide-specific phospholipase C
HMMPfam IPR015359 226 317 PF09279 EF-hand-like
IPR000909 319 470 PF00388 Phosphatidylinositol-specific phospholipase C
IPR001711 541 658 PF00387 Phosphatidylinositol-specific phospholipase C
IPR000008 680 763 PF00168 C2 calcium-dependent membrane targeting
IPR014815 999 1191 PF08703 PLC-beta
HMMSmart IPR000909 318 469 SM00148 Phosphatidylinositol-specific phospholipase C
IPR001711 542 658 SM00149 Phosphatidylinositol-specific phospholipase C
IPR000008 679 778 SM00239 C2 calcium-dependent membrane targeting
ProfileScan IPR000909 318 469 PS50007 Phosphatidylinositol-specific phospholipase C
IPR001711 542 658 PS50008 Phosphatidylinositol-specific phospholipase C
IPR000008 665 763 PS50004 C2 calcium-dependent membrane targeting
Expression profile
Description

RT-PCR
Experimental conditions
Primer_f TGATGGATTGGACGTTGTGAC
Primer_r CAAGTGGAAGAAACATCATGC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 20
Experimental conditions
Panel name GeneBridge 4
Primer_f AGGGCCATTTGCTTGCATTAC
Primer_r TCACAACGTCCAATCCATCAC
PCR product length 85 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
Order Kazusa clone(s) from : Japan || Other countries
Back to the HUGE Protein Database homepage
Send a message to office AT kazusa.or.jp