KIAA1465

Product ID	ORK00844
Accession No	AB040898
Description	immunoglobulin superfamily containing leucine-rich repeat 2, transcript variant 2
Clone name	fh13187
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4815 bp) Predicted protein sequence (785 aa)
HaloTag ORF Clone	FHC00844
Flexi ORF Clone	FXC00844
Source	Human fetal brain
Rouge ID	mKIAA1465 by Kazusa Mouse cDNA Project
Note	We replaced fj00601, former representative clones for KIAA1465 with fh13187. (2002/5/10)

Length: 4815 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	1808 bp
Genome contig ID	gi51511731f_72108768
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- CGCGTCTGTATGCAGTCAATAAAACAATCGATTTG
Flanking genome sequence (107428 - 107477)	----+--------+--------+--------+--------+----* AACTGGGCTCGGTGACTTTTTGTCAGGGGACAGAGGGAGGAGGCCTGGGC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	15	f	72208768	72216194	4	100.0	Perfect prediction

Length: 785 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q6UXK2	100.0	Immunoglobulin ...	Homo sapiens
BAF83156	99.9	unnamed protein...	Homo sapiens
BAG53336	99.9	unnamed protein...	Homo sapiens
BAG52525	99.7	unnamed protein...	Homo sapiens
XP_001096467	97.5	similar to slit...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
D86983	1.8e-10	28.4	KIAA0230
AB011537	2e-09	28.6	KIAA0813
AB011538	2.1e-08	31.4	KIAA0814
AB007865	1.6e-07	26.2	KIAA0405
AB067491	6.7e-07	26.4	KIAA1904

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001611	141	154	PR00019	Leucine-rich repeat
FPrintScan	IPR001611	162	175	PR00019	Leucine-rich repeat
HMMPfam	IPR001611	92	114	PF00560	Leucine-rich repeat
	IPR001611	116	138	PF00560	Leucine-rich repeat
	IPR001611	140	162	PF00560	Leucine-rich repeat
	IPR001611	164	186	PF00560	Leucine-rich repeat
	IPR001611	188	210	PF00560	Leucine-rich repeat
	IPR000483	248	271	PF01463	Cysteine-rich flanking region
	IPR013098	281	412	PF07679	Immunoglobulin I-set
HMMSmart	IPR003591	89	113	SM00369	Leucine-rich repeat
	IPR003591	114	137	SM00369	Leucine-rich repeat
	IPR003591	138	161	SM00369	Leucine-rich repeat
	IPR003591	162	185	SM00369	Leucine-rich repeat
	IPR003591	186	209	SM00369	Leucine-rich repeat
	IPR000483	221	271	SM00082	Cysteine-rich flanking region
ProfileScan	IPR007110	273	411	PS50835	Immunoglobulin-like

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1	GHNTCVYLAWAIFPALP	17	SECONDARY	17
	2	36	ILGAAMFPLRALWLVWALLGVAG	58	PRIMARY	23
	3	633	IVAVSVFLLVLATVPLLGAACCH	655	PRIMARY	23

Primer_f	TGTTACACTGCATCGCCGACG
Primer_r	GCGTCAGCAAATCCCCATCTC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	CCR
Primer_f	TGTTACACTGCATCGCCGACG
Primer_r	GCGTCAGCAAATCCCCATCTC
PCR product length	162 bp
PCR conditions	95 °C15 sec66 °C60 sec30 cycles