Gene/Protein Characteristic Table for KIAA0677
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Product ID ORK00602
Accession No AB014577
Description lysine (K)-specific demethylase 4A
Clone name hk02635
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4417 bp)
Predicted protein sequence (1073 aa)
Flexi ORF Clone FXC00602
Source Human adult brain
Rouge ID mKIAA0677 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 4417 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Features of the protein sequence
Description

Length: 1073 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
O75164 0 100.0 JmjC domain-con...
Homo sapiens
CAH71021 0 99.9 jumonji domain ...
Homo sapiens
Q5RD88 0 99.4 JmjC domain-con...
Pongo abelii
XP_001498241 0 96.2 similar to JmjC...
Equus caballus
XP_851005 0 96.2 similar to Jumo...
Canis lupus fam...
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB018323 1.2e-94 55.2 KIAA0780
AB020683 3.9e-93 50.9 KIAA0876
D87072 4.9e-17 33.6 KIAA0234
AB033112 9.7e-14 37.5 KIAA1286
D87076 5.7e-13 34.7 KIAA0239
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR003349 24 69 PF02375 Transcription factor jumonji
IPR013129 184 300 PF02373 Transcription factor jumonji
IPR001965 744 778 PF00628 Zinc finger
HMMSmart IPR003349 22 64 SM00545 Transcription factor jumonji
IPR003347 151 317 SM00558 Transcription factor jumonji/aspartyl beta-hydroxylase
IPR001965 718 776 SM00249 Zinc finger
IPR001965 838 894 SM00249 Zinc finger
IPR002999 906 963 SM00333 Tudor
IPR002999 964 1020 SM00333 Tudor
ProfileScan IPR003349 23 65 PS51183 Transcription factor jumonji
IPR003347 151 317 PS51184 Transcription factor jumonji/aspartyl beta-hydroxylase
Expression profile
Description

RT-PCR
Experimental conditions
Primer_f CCGCTGTAGTGAAAGGACGAG
Primer_r GTTGACCCCTCTATGGACTGG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 1
Experimental conditions
Panel name GeneBridge 4
Primer_f CCGCTGTAGTGAAAGGACGAG
Primer_r GTTGACCCCTCTATGGACTGG
PCR product length 121 bp
PCR conditions 95 °C15 sec66 °C60 sec30 cycles
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