Gene/Protein Characteristic Table for KIAA0045
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Product ID ORK07212
Accession No D28476
Description thyroid hormone receptor interactor 12, transcript variant 3
Clone name ha00941
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (6428 bp)
Predicted protein sequence (2005 aa)
Source Myeloblast cell line (KG-1)
Features of the cloned cDNA sequence
Description

Length: 6428 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 340 bp
Genome contig ID gi89161199r_230240174
PolyA signal sequence
(AATAAA,-22)
+----*----+----*----+----*----+----
TCCAGGCTGGAACAATAAATGTGGCTGTGCAGTTT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AATTCTGGGCTGTGTGTATCCATCTAGCTAAGTCTGCAGTTTTCTTTCCT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 2 r 230340174 230494899 41 99.9 Perfect prediction
Features of the protein sequence
Description

Length: 2005 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q14669 0 100.0 Probable E3 ubi...
Homo sapiens
XP_001138519 0 99.9 thyroid hormone...
Pan troglodytes
EAW70903 0 100.0 thyroid hormone...
Homo sapiens
EAW70908 0 99.7 thyroid hormone...
Homo sapiens
XP_850221 0 99.1 similar to thyr...
Canis lupus fam...
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB032957 5.3e-13 30.5 KIAA1131
AB037741 2.9e-07 25.4 KIAA1320
AB046845 1e-06 25.4 KIAA1625
AB007899 1.4e-06 24.1 KIAA0439
D42055 1.6e-06 27.4 KIAA0093
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR004170 762 797 PF02825 WWE
IPR000569 1649 2005 PF00632 HECT
HMMSmart IPR000569 1616 2005 SM00119 HECT
ProfileScan IPR004170 762 849 PS50918 WWE
IPR000569 1608 2005 PS50237 HECT
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 2
Experimental conditions
Panel name Stanford G3
Primer_f TTTGATAATGAGCAGCAGAGG
Primer_r ATTACAGAGGGCAAGAAGTCA
PCR product length 155 bp
PCR conditions 95 °C15 sec66 °C60 sec30 cycles
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