Gene/Protein Characteristic Table for KIAA1131
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Product ID ORK05410
Accession No AB032957
Description HECT domain containing E3 ubiquitin protein ligase 1
Clone name pf08614
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (7320 bp)
Predicted protein sequence (2168 aa)
Source Human brain (hippocampus)
Rouge ID mKIAA1131 by Kazusa Mouse cDNA Project
Note We replaced hh01054, former representative clones for KIAA1131 with pf08614. (2005/08/06)
Features of the cloned cDNA sequence
Description

Length: 7320 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 812 bp
Genome contig ID gi51511730r_30539075
PolyA signal sequence
(ATTAAA,-19)
+----*----+----*----+----*----+----
ATGCTCAACAATAATCATTAAAATGTTTGCAGCGT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ACAGAACTGTGTCATGTGACTATTTCAATGCAGTCATCAGACTTAAGAGC

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 14 r 30639075 30708431 35 99.9 Perfect prediction
Features of the protein sequence
Description

Length: 2168 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
EAW65950 0 100.0 HECT domain con...
Homo sapiens
NP_056197 0 99.9 HECT domain con...
Homo sapiens
XP_001489913 0 99.7 HECT domain con...
Equus caballus
NP_659037 0 98.8 HECT domain con...
Mus musculus
XP_001114983 0 99.3 similar to HECT...
Macaca mulatta
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
D28476 7.2e-18 30.4 KIAA0045
AB037741 2e-11 32.9 KIAA1320
D13635 3.1e-10 32.7 KIAA0010
AB007899 3.5e-10 32.4 KIAA0439
AB002310 1e-08 33.3 KIAA0312
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR002110 18 30 PR01415 Ankyrin
IPR002110 30 42 PR01415 Ankyrin
HMMPfam IPR002110 1 16 PF00023 Ankyrin
IPR002110 17 52 PF00023 Ankyrin
IPR012919 665 798 PF07738 Sad1/UNC-like
IPR010606 835 896 PF06701 Mib_herc2
IPR000569 1737 2168 PF00632 HECT
HMMSmart IPR000569 1701 2168 SM00119 HECT
ProfileScan IPR002110 1 41 PS50297 Ankyrin
IPR000569 1709 2168 PS50237 HECT
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f GCCTCAAGAGCAATAGTATGG
Primer_r ATTCTCAGCCCATTCCATCAG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 14
Experimental conditions
Panel name GeneBridge 4
Primer_f CTTTTTAATGATGGCCTGCAC
Primer_r GCACTTAGACCAGGTTTTCCC
PCR product length 204 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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