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Order Kazusa clone(s) from : |
| Product ID | ORK00077 |
|---|---|
| Accession No | AB007899 |
| Description | neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase, transcript variant c |
| Clone name | hj00462 |
| Vector information | |
| cDNA sequence | DNA sequence (4879 bp) Predicted protein sequence (995 aa) |
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HaloTag ORF Clone |
FHC00077
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| Flexi ORF Clone | FXC00077 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0439
by Kazusa Mouse cDNA Project
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Length: 4879 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | Warning | No warning |
Length: 995 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| FPrintScan | IPR000008 | 77 | 89 | PR00360 | C2 calcium-dependent membrane targeting |
| IPR000008 | 108 | 121 | PR00360 | C2 calcium-dependent membrane targeting | |
| HMMPfam | IPR000008 | 79 | 149 | PF00168 | C2 calcium-dependent membrane targeting |
| IPR001202 | 235 | 264 | PF00397 | WW/Rsp5/WWP | |
| IPR001202 | 407 | 436 | PF00397 | WW/Rsp5/WWP | |
| IPR001202 | 519 | 548 | PF00397 | WW/Rsp5/WWP | |
| IPR001202 | 570 | 599 | PF00397 | WW/Rsp5/WWP | |
| IPR000569 | 689 | 994 | PF00632 | HECT | |
| HMMSmart | IPR000008 | 53 | 164 | SM00239 | C2 calcium-dependent membrane targeting |
| IPR001202 | 234 | 266 | SM00456 | WW/Rsp5/WWP | |
| IPR001202 | 406 | 438 | SM00456 | WW/Rsp5/WWP | |
| IPR001202 | 518 | 550 | SM00456 | WW/Rsp5/WWP | |
| IPR001202 | 569 | 601 | SM00456 | WW/Rsp5/WWP | |
| IPR000569 | 658 | 994 | SM00119 | HECT | |
| ProfileScan | IPR000008 | 69 | 149 | PS50004 | C2 calcium-dependent membrane targeting |
| IPR001202 | 233 | 266 | PS50020 | WW/Rsp5/WWP | |
| IPR001202 | 405 | 438 | PS50020 | WW/Rsp5/WWP | |
| IPR001202 | 517 | 550 | PS50020 | WW/Rsp5/WWP | |
| IPR001202 | 568 | 601 | PS50020 | WW/Rsp5/WWP | |
| IPR000569 | 660 | 994 | PS50237 | HECT | |
| ScanRegExp | IPR001202 | 239 | 264 | PS01159 | WW/Rsp5/WWP |
| IPR001202 | 411 | 436 | PS01159 | WW/Rsp5/WWP | |
| IPR001202 | 523 | 548 | PS01159 | WW/Rsp5/WWP | |
| IPR001202 | 574 | 599 | PS01159 | WW/Rsp5/WWP |
RT-PCR
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Experimental conditions| Primer_f | GGTCGTTCAACTCCCACACTG |
|---|---|
| Primer_r | ACTTGACTTGATCCTCTGAGC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 18
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | GGTCGTTCAACTCCCACACTG |
| Primer_r | ACTTGACTTGATCCTCTGAGC |
| PCR product length | 159 bp |
| PCR conditions | 95 °C 15 sec 64 °C 60 sec 30 cycles |