BCO1 ORF clone pFN21AE6748

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE6748

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Clone info.

Species

human

Clone Name

pFN21AE6748 Sequence (with vector) Restriction Map

Product ID

FHC25785

ORF Sequence

DNA sequence (1641 bp)
Protein sequence (547 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6748

Symbol

BCO1

Alias : BCDO, BCDO1, BCMO, BCMO1, BCO

Description

beta-carotene oxygenase 1

Original Clone ID

oc96748

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1641 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 547 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10934.1 0 99.8 BCO1 CCDS41716.1 1.5e-96 41.5 BCO2 CCDS8358.2 1.6e-96 41.5 BCO2 CCDS58182.1 5.1e-95 41.3 BCO2 CCDS643.1 1.5e-93 39.7 RPE65 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR004294 6 519 PF03055 Carotenoid oxygenase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_059125 547 115300,605748 99.8 100.0 XP_016878775 478 115300,605748 87.2 100.0 XP_011521411 480 115300,605748 87.6 100.0 NP_001032367 545 611740 41.7 92.0 NP_114144 579 611740 41.7 92.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6748 Download

>KIEE6748  1641 bp
ATGGATATAATATTTGGCAGGAATAGGAAAGAACAGCTGGAGCCTGTGAGGGCCAAAGTG
ACAGGCAAGATTCCAGCATGGCTGCAGGGAACCCTGCTCCGCAACGGGCCTGGGATGCAC
ACAGTTGGGGAGTCCAGATACAACCATTGGTTCGACGGCCTTGCCCTGCTCCACAGCTTC
ACCATCAGAGACGGTGAAGTCTATTACAGGAGCAAATACCTGAGAAGCGATACCTACAAC
ACCAATATTGAGGCAAACAGGATTGTGGTGTCTGAGTTTGGAACAATGGCCTATCCGGAC
CCCTGCAAAAACATATTTTCCAAAGCTTTCTCCTACTTGTCTCACACCATCCCCGATTTC
ACCGACAACTGCCTGATCAACATCATGAAGTGCGGAGAAGACTTCTACGCGACCTCAGAG
ACCAATTACATCAGGAAAATCAACCCACAGACTCTGGAAACCCTGGAGAAGGTTGATTAT
CGTAAATACGTGGCGGTAAATCTGGCAACGTCACATCCCCATTATGATGAGGCTGGAAAT
GTTCTAAACATGGGCACATCCATTGTGGAAAAGGGGAAGACAAAGTATGTGATTTTTAAG
ATCCCTGCCACAGTACCAGAGGGCAAGAAGCAGGGGAAGAGCCCCTGGAAGCACACAGAG
GTGTTCTGCTCCATCCCATCCCGCTCCCTGCTCTCCCCAAGCTACTACCACAGCTTTGGA
GTCACCGAGAACTATGTCATCTTCCTTGAGCAGCCTTTCAGGTTGGATATTCTCAAGATG
GCAACCGCATACATCCGGAGTATGAGCTGGGCCTCCTGCCTGGCTTTCCACAGGGAGGAG
AAGACTTATATCCACATCATCGACCAAAGGACCAGGCAGCCTGTGCAGACCAAGTTTTAC
ACAGACGCCATGGTGGTCTTCCATCACGTCAACGCCTACGAAGAGGACGGCTGCATCGTG
TTTGACGTCATTGCCTACGAGGACAACAGCCTCTACCAGCTCTTCTACCTGGCCAACCTG
AACCAGGACTTCAAGGAGAACTCCAGGCTCACCTCGGTCCCCACCCTCAGGAGGTTTGCC
GTGCCCCTCCACGTGGACAAGAATGCAGAAGTGGGCACAAATTTAATCAAAGTGGCATCT
ACAACAGCCACGGCCCTGAAGGAAGAAGATGGCCAAGTCTACTGCCAGCCGGAATTTCTT
TATGAAGGCTTAGAGCTTCCACGGGTCAATTATGCTCACAATGGAAAGCAATACCGATAT
GTCTTTGCTACAGGAGTTCAGTGGAGTCCAATCCCAACCAAGATAATAAAATATGACATT
CTCACAAAGTCATCCTTAAAATGGAGAGAGGACGACTGCTGGCCAGCGGAACCCCTGTTT
GTGCCCGCGCCAGGTGCCAAGGATGAGGATGACGGAGTAATCTTATCAGCCATTGTCTCT
ACTGATCCCCAAAAGCTGCCTTTTCTGCTCATTCTGGATGCCAAAAGCTTTACGGAATTG
GCCCGTGCCTCTGTTGATGTCGATATGCACATGGATCTCCATGGATTATTCATTACAGAC
ATGGACTGGGACACAAAAAAGCAGGCCGCTTCTGAGGAACAGCGGGACAGGGCTTCCGAC
TGCCACGGGGCTCCTCTGACC

ORF Protein

Cloned ORF protein sequence for pFN21AE6748 Download

>KIEE6748  547 aa
MDIIFGRNRKEQLEPVRAKVTGKIPAWLQGTLLRNGPGMHTVGESRYNHWFDGLALLHSF
TIRDGEVYYRSKYLRSDTYNTNIEANRIVVSEFGTMAYPDPCKNIFSKAFSYLSHTIPDF
TDNCLINIMKCGEDFYATSETNYIRKINPQTLETLEKVDYRKYVAVNLATSHPHYDEAGN
VLNMGTSIVEKGKTKYVIFKIPATVPEGKKQGKSPWKHTEVFCSIPSRSLLSPSYYHSFG
VTENYVIFLEQPFRLDILKMATAYIRSMSWASCLAFHREEKTYIHIIDQRTRQPVQTKFY
TDAMVVFHHVNAYEEDGCIVFDVIAYEDNSLYQLFYLANLNQDFKENSRLTSVPTLRRFA
VPLHVDKNAEVGTNLIKVASTTATALKEEDGQVYCQPEFLYEGLELPRVNYAHNGKQYRY
VFATGVQWSPIPTKIIKYDILTKSSLKWREDDCWPAEPLFVPAPGAKDEDDGVILSAIVS
TDPQKLPFLLILDAKSFTELARASVDVDMHMDLHGLFITDMDWDTKKQAASEEQRDRASD
CHGAPLT

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6748 Download

>pFN21AE6748  6346 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGATATAATATTTGGCAGGAATAGGAAAGAACA
GCTGGAGCCTGTGAGGGCCAAAGTGACAGGCAAGATTCCAGCATGGCTGCAGGGAACCCT
GCTCCGCAACGGGCCTGGGATGCACACAGTTGGGGAGTCCAGATACAACCATTGGTTCGA
CGGCCTTGCCCTGCTCCACAGCTTCACCATCAGAGACGGTGAAGTCTATTACAGGAGCAA
ATACCTGAGAAGCGATACCTACAACACCAATATTGAGGCAAACAGGATTGTGGTGTCTGA
GTTTGGAACAATGGCCTATCCGGACCCCTGCAAAAACATATTTTCCAAAGCTTTCTCCTA
CTTGTCTCACACCATCCCCGATTTCACCGACAACTGCCTGATCAACATCATGAAGTGCGG
AGAAGACTTCTACGCGACCTCAGAGACCAATTACATCAGGAAAATCAACCCACAGACTCT
GGAAACCCTGGAGAAGGTTGATTATCGTAAATACGTGGCGGTAAATCTGGCAACGTCACA
TCCCCATTATGATGAGGCTGGAAATGTTCTAAACATGGGCACATCCATTGTGGAAAAGGG
GAAGACAAAGTATGTGATTTTTAAGATCCCTGCCACAGTACCAGAGGGCAAGAAGCAGGG
GAAGAGCCCCTGGAAGCACACAGAGGTGTTCTGCTCCATCCCATCCCGCTCCCTGCTCTC
CCCAAGCTACTACCACAGCTTTGGAGTCACCGAGAACTATGTCATCTTCCTTGAGCAGCC
TTTCAGGTTGGATATTCTCAAGATGGCAACCGCATACATCCGGAGTATGAGCTGGGCCTC
CTGCCTGGCTTTCCACAGGGAGGAGAAGACTTATATCCACATCATCGACCAAAGGACCAG
GCAGCCTGTGCAGACCAAGTTTTACACAGACGCCATGGTGGTCTTCCATCACGTCAACGC
CTACGAAGAGGACGGCTGCATCGTGTTTGACGTCATTGCCTACGAGGACAACAGCCTCTA
CCAGCTCTTCTACCTGGCCAACCTGAACCAGGACTTCAAGGAGAACTCCAGGCTCACCTC
GGTCCCCACCCTCAGGAGGTTTGCCGTGCCCCTCCACGTGGACAAGAATGCAGAAGTGGG
CACAAATTTAATCAAAGTGGCATCTACAACAGCCACGGCCCTGAAGGAAGAAGATGGCCA
AGTCTACTGCCAGCCGGAATTTCTTTATGAAGGCTTAGAGCTTCCACGGGTCAATTATGC
TCACAATGGAAAGCAATACCGATATGTCTTTGCTACAGGAGTTCAGTGGAGTCCAATCCC
AACCAAGATAATAAAATATGACATTCTCACAAAGTCATCCTTAAAATGGAGAGAGGACGA
CTGCTGGCCAGCGGAACCCCTGTTTGTGCCCGCGCCAGGTGCCAAGGATGAGGATGACGG
AGTAATCTTATCAGCCATTGTCTCTACTGATCCCCAAAAGCTGCCTTTTCTGCTCATTCT
GGATGCCAAAAGCTTTACGGAATTGGCCCGTGCCTCTGTTGATGTCGATATGCACATGGA
TCTCCATGGATTATTCATTACAGACATGGACTGGGACACAAAAAAGCAGGCCGCTTCTGA
GGAACAGCGGGACAGGGCTTCCGACTGCCACGGGGCTCCTCTGACCGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT