(Circular) (Six-base) MAPSORT of: pFN21AE6748.seq Check: 8054 from: 1 to: 6346 pFN21AE6748 6346 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2973 4156 278 Size: 53 83 186 936 1437 1183 2468 Fragments arranged by size: 2468 1437 1183 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3183 3689 1937 Size: 1246 506 4594 Fragments arranged by size: 4594 1246 506 Acc65I G'GTAC_C Cuts at: 3667 3667 Size: 6346 AclI AA'CG_TT Cuts at: 1563 4473 4846 1563 Size: 2910 373 3063 Fragments arranged by size: 3063 2910 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3182 4409 5793 6061 1632 Size: 1550 1227 1384 268 1917 Fragments arranged by size: 1917 1550 1384 1227 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6149 AflIII A'CryG_T Cuts at: 1203 5245 6222 1203 Size: 4042 977 1327 Fragments arranged by size: 4042 1327 977 AgeI A'CCGG_T Cuts at: 5177 5177 Size: 6346 AhdI GACnn_n'nnGTC Cuts at: 5075 5075 Size: 6346 AleI CACnn'nnGTG Cuts at: 1166 2663 1166 Size: 1497 4849 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6346 AlwNI CAG_nnn'CTG Cuts at: 2458 4089 5661 2458 Size: 1631 1572 3143 Fragments arranged by size: 3143 1631 1572 ApaLI G'TGCA_C Cuts at: 4403 5559 4403 Size: 1156 5190 ApoI r'AATT_y Cuts at: 1493 3124 3197 3655 3881 4033 6142 1493 Size: 1631 73 458 226 152 2109 1697 Fragments arranged by size: 2109 1697 1631 458 226 152 73 AseI AT'TA_AT Cuts at: 160 1032 4900 160 Size: 872 3868 1606 Fragments arranged by size: 3868 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6346 AvaI C'yCGr_G Cuts at: 1870 1943 3671 1870 Size: 73 1728 4545 Fragments arranged by size: 4545 1728 73 BamHI G'GATC_C Cuts at: 3676 4043 3676 Size: 367 5979 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3398 3667 5122 618 Size: 325 217 627 1611 269 1455 1842 Fragments arranged by size: 1842 1611 1455 627 325 269 217 BanII G_rGCy'C Cuts at: 1391 2648 3637 3665 1391 Size: 1257 989 28 4072 Fragments arranged by size: 4072 1257 989 28 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2409 2588 2852 2914 928 Size: 971 510 179 264 62 4360 Fragments arranged by size: 4360 971 510 264 179 62 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2147 2181 4537 4571 1903 Size: 34 210 34 2356 34 3678 Fragments arranged by size: 3678 2356 210 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4238 5448 4238 Size: 1210 5136 BclI T'GATC_A Cuts at: 1548 2379 1548 Size: 831 5515 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4957 136 Size: 107 122 71 631 605 3285 1525 Fragments arranged by size: 3285 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6341 6341 Size: 6346 BlpI GC'TnA_GC Cuts at: 3763 3763 Size: 6346 Bme1580I G_kGCm'C Cuts at: 1790 2561 3090 3122 3390 4407 5563 1790 Size: 771 529 32 268 1017 1156 2573 Fragments arranged by size: 2573 1156 1017 771 529 268 32 BmgBI CAC'GTC Cuts at: 1318 2932 1318 Size: 1614 4732 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3580 4077 5035 Size: 416 53 83 399 24 2163 497 958 Cuts at: 5035 442 Size: 1753 Fragments arranged by size: 2163 1753 958 497 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6346 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 2489 3031 5006 1239 Size: 824 426 542 1975 2579 Fragments arranged by size: 2579 1975 824 542 426 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2762 2771 4471 5336 5634 2762 Size: 9 1700 865 298 3474 Fragments arranged by size: 3474 1700 865 298 9 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2417 5009 882 Size: 617 918 2592 2219 Fragments arranged by size: 2592 2219 918 617 BsaAI yAC'GTr Cuts at: 493 1204 2494 3097 4137 493 Size: 711 1290 603 1040 2702 Fragments arranged by size: 2702 1290 1040 711 603 BsaBI GATnn'nnATC Cuts at: 2384 6340 2384 Size: 3956 2390 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2910 2970 4153 4535 Size: 53 83 186 936 1377 60 1183 382 Cuts at: 4535 5170 275 Size: 635 1451 Fragments arranged by size: 1451 1377 1183 936 635 382 186 83 60 53 BsaWI w'CCGG_w Cuts at: 2299 2799 4779 5177 5451 5598 6009 2299 Size: 500 1980 398 274 147 411 2636 Fragments arranged by size: 2636 1980 500 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6121 Fragments arranged by size: 6121 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2855 3627 1186 Size: 1669 772 3905 Fragments arranged by size: 3905 1669 772 BseYI C'CCAG_C Cuts at: 2811 5549 6045 2811 Size: 2738 496 3112 Fragments arranged by size: 3112 2738 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2909 1163 Size: 573 1173 4600 Fragments arranged by size: 4600 1173 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3796 4557 5585 1540 Size: 275 186 1795 761 1028 2301 Fragments arranged by size: 2301 1795 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4407 4492 5563 4407 Size: 85 1071 5190 Fragments arranged by size: 5190 1071 85 BsmI GAATG_Cn' Cuts at: 3112 3857 3950 3112 Size: 745 93 5508 Fragments arranged by size: 5508 745 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2187 2187 Size: 6346 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2561 2648 3090 3122 3390 3637 3665 Size: 399 771 87 442 32 268 247 28 Cuts at: 3665 4407 4492 5563 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 771 742 442 399 268 247 87 85 32 28 BspEI T'CCGG_A Cuts at: 2299 2799 6009 2299 Size: 500 3210 2636 Fragments arranged by size: 3210 2636 500 BspHI T'CATG_A Cuts at: 2388 4235 5965 2388 Size: 1847 1730 2769 Fragments arranged by size: 2769 1847 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3701 844 Size: 2857 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2688 4233 1394 Size: 429 865 1545 3507 Fragments arranged by size: 3507 1545 865 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2973 4841 5015 65 Size: 1183 1725 1868 174 1396 Fragments arranged by size: 1868 1725 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4990 5177 1700 Size: 110 3180 187 2869 Fragments arranged by size: 3180 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6346 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6346 BssSI C'ACGA_G Cuts at: 4406 5418 4406 Size: 1012 5334 BstAPI GCAn_nnn'nTGC Cuts at: 794 2887 794 Size: 2093 4253 BstBI TT'CG_AA Cuts at: 5150 5150 Size: 6346 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6346 BstXI CCAn_nnnn'nTGG Cuts at: 2720 2720 Size: 6346 BstYI r'GATC_y Cuts at: 1972 2604 3538 3676 4043 4428 4445 5897 5983 Size: 632 934 138 367 385 17 1452 86 Cuts at: 5983 5995 6341 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 934 632 385 367 346 138 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3071 3071 Size: 6346 BtgI C'CryG_G Cuts at: 513 1065 2004 2913 3153 3224 3544 3628 513 Size: 552 939 909 240 71 320 84 3231 Fragments arranged by size: 3231 939 909 552 320 240 84 71 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5607 Fragments arranged by size: 5607 683 56 BtsI GCAGTG_nn' Cuts at: 3862 4656 4676 3862 Size: 794 20 5532 Fragments arranged by size: 5532 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6346 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6311 DraI TTT'AAA Cuts at: 3650 3650 Size: 6346 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2201 5353 809 Size: 507 885 3152 1802 Fragments arranged by size: 3152 1802 885 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3015 3175 3365 Size: 54 1425 50 275 18 1185 160 190 Cuts at: 3365 3793 4682 6311 8 Size: 428 889 1629 43 Fragments arranged by size: 1629 1425 1185 889 428 275 190 160 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3793 1537 Size: 275 1981 4090 Fragments arranged by size: 4090 1981 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2636 2940 3011 4276 1353 Size: 1283 304 71 1265 3423 Fragments arranged by size: 3423 1283 1265 304 71 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4933 5307 5453 387 Size: 1064 138 3344 374 146 1280 Fragments arranged by size: 3344 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2489 3031 3182 4409 5006 5793 Size: 393 431 426 542 151 1227 597 787 Cuts at: 5793 6061 1239 Size: 268 1524 Fragments arranged by size: 1524 1227 787 597 542 431 426 393 268 151 EcoNI CCTnn'n_nnAGG Cuts at: 2471 3026 2471 Size: 555 5791 EcoRI G'AATT_C Cuts at: 3655 4033 3655 Size: 378 5968 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3355 3355 Size: 6346 FspI TGC'GCA Cuts at: 4852 4852 Size: 6346 HaeII r_GCGC'y Cuts at: 1678 5493 1678 Size: 3815 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4987 5019 5061 5093 1373 Size: 32 135 32 3415 32 42 32 2626 Fragments arranged by size: 3415 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2935 3690 3936 669 Size: 988 281 997 755 246 3079 Fragments arranged by size: 3079 997 988 755 281 246 HindIII A'AGCT_T Cuts at: 748 2328 3490 748 Size: 1580 1162 3604 Fragments arranged by size: 3604 1580 1162 HpaI GTT'AAC Cuts at: 3936 3936 Size: 6346 KpnI G_GTAC'C Cuts at: 3671 3671 Size: 6346 MfeI C'AATT_G Cuts at: 3945 3945 Size: 6346 MluI A'CGCG_T Cuts at: 6222 6222 Size: 6346 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2748 3645 5460 5644 1305 Size: 1443 897 1815 184 2007 Fragments arranged by size: 2007 1815 1443 897 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3017 3177 3367 10 Size: 54 1425 343 1185 160 190 2989 Fragments arranged by size: 2989 1425 1185 343 190 160 54 MslI CAynn'nnrTG Cuts at: 518 1166 2529 2663 4304 4663 4822 518 Size: 648 1363 134 1641 359 159 2042 Fragments arranged by size: 2042 1641 1363 648 359 159 134 MspA1I CmG'CkG Cuts at: 1606 2041 3371 3607 3762 4439 5587 5832 1606 Size: 435 1330 236 155 677 1148 245 2120 Fragments arranged by size: 2120 1330 1148 677 435 245 236 155 NcoI C'CATG_G Cuts at: 513 1065 2004 2913 3544 513 Size: 552 939 909 631 3315 Fragments arranged by size: 3315 939 909 631 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6346 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6346 NotI GC'GGCC_GC Cuts at: 3793 3793 Size: 6346 NspI r_CATG'y Cuts at: 1132 1229 3704 5249 1132 Size: 97 2475 1545 2229 Fragments arranged by size: 2475 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6346 PciI A'CATG_T Cuts at: 5245 5245 Size: 6346 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6346 PmeI GTTT'AAAC Cuts at: 3650 3650 Size: 6346 PmlI CAC'GTG Cuts at: 3097 3097 Size: 6346 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4415 4447 5928 5960 4415 Size: 32 1481 32 4801 Fragments arranged by size: 4801 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6346 PsiI TTA'TAA Cuts at: 3916 3916 Size: 6346 PstI C_TGCA'G Cuts at: 830 2091 3698 830 Size: 1261 1607 3478 Fragments arranged by size: 3478 1607 1261 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6346 PvuII CAG'CTG Cuts at: 2041 2041 Size: 6346 SalI G'TCGA_C Cuts at: 1936 3688 1936 Size: 1752 4594 SapI GCTCTTCn'nnn_ Cuts at: 2636 3011 2636 Size: 375 5971 SbfI CC_TGCA'GG Cuts at: 3698 3698 Size: 6346 ScaI AGT'ACT Cuts at: 4594 6306 4594 Size: 1712 4634 SfcI C'TryA_G Cuts at: 826 1045 2087 3273 3694 4829 5510 5701 826 Size: 219 1042 1186 421 1135 681 191 1471 Fragments arranged by size: 1471 1186 1135 1042 681 421 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6346 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6346 SmaI CCC'GGG Cuts at: 1872 3673 1872 Size: 1801 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2750 2777 4450 5351 5613 820 Size: 197 926 807 27 1673 901 262 1553 Fragments arranged by size: 1673 1553 926 901 807 262 197 27 SnaBI TAC'GTA Cuts at: 493 4137 493 Size: 3644 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6346 SphI G_CATG'C Cuts at: 1132 3704 1132 Size: 2572 3774 SspI AAT'ATT Cuts at: 5 52 2016 2251 4270 5 Size: 47 1964 235 2019 2081 Fragments arranged by size: 2081 2019 1964 235 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6346 StyI C'CwwG_G Cuts at: 513 1065 2004 2913 3402 3544 3785 513 Size: 552 939 909 489 142 241 3074 Fragments arranged by size: 3074 939 909 552 489 241 142 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3049 4373 4390 4543 4728 5173 325 Size: 1255 1469 1324 17 153 185 445 1498 Fragments arranged by size: 1498 1469 1324 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4592 6304 96 Size: 274 80 33 51 1443 2615 1712 138 Fragments arranged by size: 2615 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6306 6338 6306 Size: 32 6314 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6346 XbaI T'CTAG_A Cuts at: 3682 3682 Size: 6346 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6346 XmaI C'CCGG_G Cuts at: 1870 3671 1870 Size: 1801 4545 XmnI GAAnn'nnTTC Cuts at: 4475 4475 Size: 6346 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2971 4154 276 Size: 53 83 186 936 1437 1183 2468 Fragments arranged by size: 2468 1437 1183 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI