(Linear) (Six-base) MAPSORT of: KIEE6748.seq Check: 4872 from: 1 to: 1641 KIEE6748.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AatII G_ACGT'C Cuts at: 0 968 1641 Size: 968 673 AccI GT'mk_AC Cuts at: 0 1178 1641 Size: 1178 463 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1177 1641 Size: 1177 464 AleI CACnn'nnGTG Cuts at: 0 658 1641 Size: 658 983 AlwNI CAG_nnn'CTG Cuts at: 0 453 1641 Size: 453 1188 ApoI r'AATT_y Cuts at: 0 1119 1192 1641 Size: 1119 73 449 Fragments arranged by size: 1119 449 73 BanI G'GyrC_C Cuts at: 0 1393 1641 Size: 1393 248 BanII G_rGCy'C Cuts at: 0 643 1632 1641 Size: 643 989 9 Fragments arranged by size: 989 643 9 BbsI GAAGACnn'nnnn_ Cuts at: 0 404 583 847 909 1641 Size: 404 179 264 62 732 Fragments arranged by size: 732 404 264 179 62 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 142 176 1641 Size: 142 34 1465 Fragments arranged by size: 1465 142 34 BclI T'GATC_A Cuts at: 0 374 1641 Size: 374 1267 Bme1580I G_kGCm'C Cuts at: 0 556 1085 1117 1385 1641 Size: 556 529 32 268 256 Fragments arranged by size: 556 529 268 256 32 BmgBI CAC'GTC Cuts at: 0 927 1641 Size: 927 714 BmrI ACTGGGnnnn_n' Cuts at: 0 1575 1641 Size: 1575 66 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 484 1026 1641 Size: 58 426 542 615 Fragments arranged by size: 615 542 426 58 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 757 766 1641 Size: 757 9 875 Fragments arranged by size: 875 757 9 BsaI GGTCTCn'nnnn_ Cuts at: 0 412 1641 Size: 412 1229 BsaAI yAC'GTr Cuts at: 0 489 1092 1641 Size: 489 603 549 Fragments arranged by size: 603 549 489 BsaBI GATnn'nnATC Cuts at: 0 379 1641 Size: 379 1262 BsaHI Gr'CG_yC Cuts at: 0 905 965 1641 Size: 905 60 676 Fragments arranged by size: 905 676 60 BsaWI w'CCGG_w Cuts at: 0 294 794 1641 Size: 294 500 847 Fragments arranged by size: 847 500 294 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 850 1622 1641 Size: 850 772 19 Fragments arranged by size: 850 772 19 BseYI C'CCAG_C Cuts at: 0 806 1641 Size: 806 835 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 904 1641 Size: 904 737 BsmI GAATG_Cn' Cuts at: 0 1107 1641 Size: 1107 534 BsmBI CGTCTCn'nnnn_ Cuts at: 0 182 1641 Size: 182 1459 Bsp1286I G_dGCh'C Cuts at: 0 556 643 1085 1117 1385 1632 1641 Size: 556 87 442 32 268 247 9 Fragments arranged by size: 556 442 268 247 87 32 9 BspEI T'CCGG_A Cuts at: 0 294 794 1641 Size: 294 500 847 Fragments arranged by size: 847 500 294 BspHI T'CATG_A Cuts at: 0 383 1641 Size: 383 1258 BsrBI CCG'CTC Cuts at: 0 683 1641 Size: 683 958 BsrDI GCAATG_nn' Cuts at: 0 968 1641 Size: 968 673 BstAPI GCAn_nnn'nTGC Cuts at: 0 882 1641 Size: 882 759 BstXI CCAn_nnnn'nTGG Cuts at: 0 715 1641 Size: 715 926 BstYI r'GATC_y Cuts at: 0 599 1533 1641 Size: 599 934 108 Fragments arranged by size: 934 599 108 Bsu36I CC'TnA_GG Cuts at: 0 1066 1641 Size: 1066 575 BtgI C'CryG_G Cuts at: 0 908 1148 1219 1539 1623 1641 Size: 908 240 71 320 84 18 Fragments arranged by size: 908 320 240 84 71 18 DrdI GACnn_nn'nnGTC Cuts at: 0 196 1641 Size: 196 1445 EaeI y'GGCC_r Cuts at: 0 1010 1170 1360 1641 Size: 1010 160 190 281 Fragments arranged by size: 1010 281 190 160 EarI CTCTTCn'nnn_ Cuts at: 0 631 935 1006 1641 Size: 631 304 71 635 Fragments arranged by size: 635 631 304 71 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 484 1026 1177 1641 Size: 58 426 542 151 464 Fragments arranged by size: 542 464 426 151 58 EcoNI CCTnn'n_nnAGG Cuts at: 0 466 1021 1641 Size: 466 555 620 Fragments arranged by size: 620 555 466 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1350 1641 Size: 1350 291 HincII GTy'rAC Cuts at: 0 930 1641 Size: 930 711 HindIII A'AGCT_T Cuts at: 0 323 1485 1641 Size: 323 1162 156 Fragments arranged by size: 1162 323 156 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 743 1640 1641 Size: 743 897 1 Fragments arranged by size: 897 743 1 MscI TGG'CCA Cuts at: 0 1012 1172 1362 1641 Size: 1012 160 190 279 Fragments arranged by size: 1012 279 190 160 MslI CAynn'nnrTG Cuts at: 0 524 658 1641 Size: 524 134 983 Fragments arranged by size: 983 524 134 MspA1I CmG'CkG Cuts at: 0 36 1366 1602 1641 Size: 36 1330 236 39 Fragments arranged by size: 1330 236 39 36 NcoI C'CATG_G Cuts at: 0 908 1539 1641 Size: 908 631 102 Fragments arranged by size: 908 631 102 PmlI CAC'GTG Cuts at: 0 1092 1641 Size: 1092 549 PstI C_TGCA'G Cuts at: 0 86 1641 Size: 86 1555 PvuII CAG'CTG Cuts at: 0 36 1641 Size: 36 1605 SapI GCTCTTCn'nnn_ Cuts at: 0 631 1006 1641 Size: 631 375 635 Fragments arranged by size: 635 631 375 SfcI C'TryA_G Cuts at: 0 82 1268 1641 Size: 82 1186 373 Fragments arranged by size: 1186 373 82 SmlI C'TyrA_G Cuts at: 0 745 772 1641 Size: 745 27 869 Fragments arranged by size: 869 745 27 SspI AAT'ATT Cuts at: 0 11 246 1641 Size: 11 235 1395 Fragments arranged by size: 1395 235 11 StyI C'CwwG_G Cuts at: 0 908 1397 1539 1641 Size: 908 489 142 102 Fragments arranged by size: 908 489 142 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1044 1641 Size: 1044 597 ZraI GAC'GTC Cuts at: 0 966 1641 Size: 966 675 Enzymes that do cut: AatII AccI AcuI AleI AlwNI ApoI BanI BanII BbsI BcgI BclI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BstAPI BstXI BstYI Bsu36I BtgI DrdI EaeI EarI Eco57MI EcoNI FalI HincII HindIII MmeI MscI MslI MspA1I NcoI PmlI PstI PvuII SapI SfcI SmlI SspI StyI TaqII ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BciVI BfrBI BglI BglII BlpI BmtI BplI Bpu10I BsaXI BsiEI BsiHKAI BsiWI BspMI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI