################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## Acc65I AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BbeI BbvCI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsiEI BsiHKAI BsiWI BsoBI Bsp1407I BspDI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DraII DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HpaI KasI KpnI KspI MaeI MfeI MluI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 964 GACGTC GACGT^C AccI 1177 GTCTAC GT^[AC][GT]AC AfaI 613 GTAC GT^AC AlwNI 448 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1065 CCTCAGG CC^T[ACGT]AGG BanI 1393 GGTGCC G^G[CT][GA]CC BbrPI 1090 CACGTG CAC^GTG BcgI 153 CGACGGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 374 TGATCA T^GATCA BsaBI 375 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 418 GAGACC GGTCTC(1/5) BsgI 883 GTGCAG GTGCAG(16/14) BsmBI 188 GAGACG CGTCTC(1/5) BsmI 1101 GAATGC GAATGC(1/-1) BspHI 383 TCATGA T^CATGA BsrBI 681 CCGCTC CCGCTC(-3/-3) BsrDI 969 CATTGC GCAATG(2/0) BsrI 1565 ACTGG ACTGG(1/-1) BstAPI 876 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 708 CCACAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1065 CCTCAGG CC^T[ACGT]AGG CfoI 1387 GCGC GCG^C DrdI 190 GACGGTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1065 CCTCAGG CC^T[ACGT]AGG FbaI 374 TGATCA T^GATCA HgaI 904 GACGC GACGC(5/10) HhaI 1387 GCGC GCG^C HinP1I 1387 GCGC G^CGC HincII 928 GTCAAC GT[CT]^[GA]AC HindII 928 GTCAAC GT[CT]^[GA]AC MamI 375 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PmaCI 1090 CACGTG CAC^GTG PmlI 1090 CACGTG CAC^GTG PstI 82 CTGCAG CTGCA^G PvuII 34 CAGCTG CAG^CTG RcaI 383 TCATGA T^CATGA RsaI 613 GTAC GT^AC TfiI 69 GATTC G^A[AT]TC TspRI 1279 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 408 CGCG CG^CG 1386 CGCG AccIII 294 TCCGGA T^CCGGA 794 TCCGGA AcsI 1119 AAATTT [GA]^AATT[CT] 1192 GAATTT AcyI 904 GACGCC G[GA]^CG[CT]C 964 GACGTC ApoI 1119 AAATTT [GA]^AATT[CT] 1192 GAATTT BanII 639 GAGCCC G[GA]GC[CT]^C 1628 GGGCTC BsaAI 487 TACGTG [CT]AC^GT[GA] 1090 CACGTG BsaHI 904 GACGCC G[GA]^CG[CT]C 964 GACGTC BsaWI 294 TCCGGA [AT]^CCGG[AT] 794 TCCGGA BseAI 294 TCCGGA T^CCGGA 794 TCCGGA BseRI 1631 CTCCTC GAGGAG(10/8) 835 GAGGAG BspEI 294 TCCGGA T^CCGGA 794 TCCGGA BstUI 408 CGCG CG^CG 1386 CGCG BstYI 599 AGATCC [GA]^GATC[CT] 1533 GGATCT EcoNI 462 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1017 CCTGAACCAGG Hin1I 904 GACGCC G[GA]^CG[CT]C 964 GACGTC HindIII 323 AAGCTT A^AGCTT 1485 AAGCTT MflI 599 AGATCC [GA]^GATC[CT] 1533 GGATCT MroI 294 TCCGGA T^CCGGA 794 TCCGGA MslI 520 CATTATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 654 CACAGAGGTG MvnI 408 CGCG CG^CG 1386 CGCG NcoI 908 CCATGG C^CATGG 1539 CCATGG SapI 636 GAAGAGC GCTCTTC(1/4) 999 GCTCTTC SfcI 82 CTGCAG C^T[GA][CT]AG 1268 CTACAG SmlI 745 CTTGAG C^T[CT][GA]AG 772 CTCAAG SspI 9 AATATT AAT^ATT 244 AATATT XhoII 599 AGATCC [GA]^GATC[CT] 1533 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AluI AlwI AvaII BalI BbsI BbvI BmyI BpmI BpuAI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI StyI TaqI Tru9I TseI Tsp45I Tsp509I TthHB8I