RHBDL1 ORF clone pFN21AE6393

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Halotag ORF Clone pFN21AE6393

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Clone info.

Species

human

Clone Name

pFN21AE6393 Sequence (with vector) Restriction Map

Product ID

FHC25531

ORF Sequence

DNA sequence (1314 bp)
Protein sequence (438 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6393

Symbol

RHBDL1

Alias : RHBDL, RRP

Description

rhomboid, veinlet-like 1 (Drosophila)

Original Clone ID

oc96393

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1314 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 438 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS61779.1 4e-128 100.0 RHBDL1 CCDS82103.1 1.4e-74 54.2 RHBDL3 CCDS32613.1 1.6e-74 56.6 RHBDL3 CCDS30680.1 2.9e-28 38.7 RHBDL2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR022764 241 394 PF01694 Peptidase S54
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 271 IGVPLEMVHGLLRISLLYLAGVL 293 PRIMARY 23 2 334 RCPYKLLRMVLALVCMSSEVGRA 356 SECONDARY 23 3 373 SFMAHLAGAVVGVSMGLTILRSY 395 SECONDARY 23 4 409 LLAYGTFLLFAVFWNVFAYDLLG 431 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001305662 438 603264 100.0 100.0 NP_001265649 373 603264 100.0 82.4 XP_016879338 469 603264 74.0 92.5 NP_001265650 298 603264 79.2 82.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6393 Download

>KIEE6393  1314 bp
ATGGGTAGGGTGGAAGACGGGGGAACAACTGAGGAGCTGGAGGACTGGGACCCAGGCACC
AGTGCCCTGCCAGCTCCTGGGATCAAGCAGGGTCCCAGGGAACAGACAGGCACGGGGCCC
CTGTCCCAAAAGTGCTGGGAGCCTGAGCCTGATGCTCCCAGCCAGCCTGGCCCAGCCCTT
TGGTCCAGGGGTCGGGCCCGCACTCAGGCCTTGGCTGGCGGCTCCTCACTGCAGCAGCTG
GACCCCGAGAACACAGGCTTCATCGGTGCGGACACCTTCACTGGCCTGGTGCACAGCCAT
GAGCTGCCCCTGGACCCGGCCAAGCTGGACATGCTGGTGGCCCTGGCTCAGAGCAACGAG
CAGGGCCAGGTCTGCTACCAGGAGCTGGTGGACCTGATCAGCAGCAAGCGCTCCAGCAGT
TTCAAGCGGGCCATTGCTAACGGACAGCGGGCACTGCCCCGGGACGGGCCGCTGGATGAG
CCAGGCCTAGGTGTCTACAAGCGGTTTGTGCGTTACGTGGCCTACGAGATCCTGCCTTGT
GAGGTGGACCGCCGCTGGTACTTCTACCGTCACCGCAGCTGCCCACCCCCCGTGTTCATG
GCCTCGGTCACTCTTGCCCAGATCATCGTGTTCCTGTGTTACGGGGCCCGCCTCAACAAG
TGGGTGCTGCAGACCTACCACCCCGAGTACATGAAGAGCCCCCTTGTGTACCACCCCGGG
CACCGTGCCCGCGCCTGGCGCTTCCTCACCTACATGTTCATGCACGTTGGGCTGGAGCAG
CTGGGGTTCAACGCCCTCCTGCAGCTGATGATCGGGGTGCCCCTGGAGATGGTGCACGGC
CTGCTCCGCATCAGCCTGCTCTACCTGGCAGGCGTGCTGGCAGGCTCCCTAACCGTCTCC
ATCACCGACATGCGGGCCCCGGTGGTGGGAGGCTCCGGCGGGGTCTACGCCCTGTGCTCG
GCACACCTGGCCAACGTTGTCATGAACTGGGCTGGGATGAGATGTCCCTACAAGTTGCTG
AGGATGGTGCTGGCCTTGGTGTGCATGAGCTCCGAGGTGGGCCGGGCCGTGTGGCTGCGC
TTCTCCCCGCCGCTGCCCGCCTCGGGCCCACAGCCCAGCTTCATGGCGCACCTGGCAGGC
GCGGTGGTGGGGGTGAGCATGGGCCTGACCATCCTGCGGAGCTACGAGGAGCGCCTGCGG
GACCAGTGCGGCTGGTGGGTGGTGCTGCTGGCCTACGGCACCTTCCTGCTCTTCGCCGTC
TTCTGGAACGTCTTCGCCTACGACCTGCTGGGCGCCCACATCCCCCCACCGCCC

ORF Protein

Cloned ORF protein sequence for pFN21AE6393 Download

>KIEE6393  438 aa
MGRVEDGGTTEELEDWDPGTSALPAPGIKQGPREQTGTGPLSQKCWEPEPDAPSQPGPAL
WSRGRARTQALAGGSSLQQLDPENTGFIGADTFTGLVHSHELPLDPAKLDMLVALAQSNE
QGQVCYQELVDLISSKRSSSFKRAIANGQRALPRDGPLDEPGLGVYKRFVRYVAYEILPC
EVDRRWYFYRHRSCPPPVFMASVTLAQIIVFLCYGARLNKWVLQTYHPEYMKSPLVYHPG
HRARAWRFLTYMFMHVGLEQLGFNALLQLMIGVPLEMVHGLLRISLLYLAGVLAGSLTVS
ITDMRAPVVGGSGGVYALCSAHLANVVMNWAGMRCPYKLLRMVLALVCMSSEVGRAVWLR
FSPPLPASGPQPSFMAHLAGAVVGVSMGLTILRSYEERLRDQCGWWVVLLAYGTFLLFAV
FWNVFAYDLLGAHIPPPP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6393 Download

>pFN21AE6393  6019 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGTAGGGTGGAAGACGGGGGAACAACTGAGGA
GCTGGAGGACTGGGACCCAGGCACCAGTGCCCTGCCAGCTCCTGGGATCAAGCAGGGTCC
CAGGGAACAGACAGGCACGGGGCCCCTGTCCCAAAAGTGCTGGGAGCCTGAGCCTGATGC
TCCCAGCCAGCCTGGCCCAGCCCTTTGGTCCAGGGGTCGGGCCCGCACTCAGGCCTTGGC
TGGCGGCTCCTCACTGCAGCAGCTGGACCCCGAGAACACAGGCTTCATCGGTGCGGACAC
CTTCACTGGCCTGGTGCACAGCCATGAGCTGCCCCTGGACCCGGCCAAGCTGGACATGCT
GGTGGCCCTGGCTCAGAGCAACGAGCAGGGCCAGGTCTGCTACCAGGAGCTGGTGGACCT
GATCAGCAGCAAGCGCTCCAGCAGTTTCAAGCGGGCCATTGCTAACGGACAGCGGGCACT
GCCCCGGGACGGGCCGCTGGATGAGCCAGGCCTAGGTGTCTACAAGCGGTTTGTGCGTTA
CGTGGCCTACGAGATCCTGCCTTGTGAGGTGGACCGCCGCTGGTACTTCTACCGTCACCG
CAGCTGCCCACCCCCCGTGTTCATGGCCTCGGTCACTCTTGCCCAGATCATCGTGTTCCT
GTGTTACGGGGCCCGCCTCAACAAGTGGGTGCTGCAGACCTACCACCCCGAGTACATGAA
GAGCCCCCTTGTGTACCACCCCGGGCACCGTGCCCGCGCCTGGCGCTTCCTCACCTACAT
GTTCATGCACGTTGGGCTGGAGCAGCTGGGGTTCAACGCCCTCCTGCAGCTGATGATCGG
GGTGCCCCTGGAGATGGTGCACGGCCTGCTCCGCATCAGCCTGCTCTACCTGGCAGGCGT
GCTGGCAGGCTCCCTAACCGTCTCCATCACCGACATGCGGGCCCCGGTGGTGGGAGGCTC
CGGCGGGGTCTACGCCCTGTGCTCGGCACACCTGGCCAACGTTGTCATGAACTGGGCTGG
GATGAGATGTCCCTACAAGTTGCTGAGGATGGTGCTGGCCTTGGTGTGCATGAGCTCCGA
GGTGGGCCGGGCCGTGTGGCTGCGCTTCTCCCCGCCGCTGCCCGCCTCGGGCCCACAGCC
CAGCTTCATGGCGCACCTGGCAGGCGCGGTGGTGGGGGTGAGCATGGGCCTGACCATCCT
GCGGAGCTACGAGGAGCGCCTGCGGGACCAGTGCGGCTGGTGGGTGGTGCTGCTGGCCTA
CGGCACCTTCCTGCTCTTCGCCGTCTTCTGGAACGTCTTCGCCTACGACCTGCTGGGCGC
CCACATCCCCCCACCGCCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT