Restriction map for
pFN21AE6393
(Linear) (Six-base) MAPSORT of: KIEE6393.seq Check: 2435 from: 1 to: 1314
KIEE6393.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AccI GT'mk_AC
Cuts at: 0 494 944 1314
Size: 494 450 370
Fragments arranged by size:
494 450 370
AclI AA'CG_TT
Cuts at: 0 974 1314
Size: 974 340
AfeI AGC'GCT
Cuts at: 0 409 1314
Size: 409 905
AflIII A'CryG_T
Cuts at: 0 752 1314
Size: 752 562
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 871 1261 1314
Size: 871 390 53
Fragments arranged by size:
871 390 53
AlwNI CAG_nnn'CTG
Cuts at: 0 76 237 384 1314
Size: 76 161 147 930
Fragments arranged by size:
930 161 147 76
ApaI G_GGCC'C
Cuts at: 0 119 198 648 918 1108 1314
Size: 119 79 450 270 190 206
Fragments arranged by size:
450 270 206 190 119 79
ApaLI G'TGCA_C
Cuts at: 0 289 832 1314
Size: 289 543 482
Fragments arranged by size:
543 482 289
AvaI C'yCGr_G
Cuts at: 0 244 458 682 715 1101 1314
Size: 244 214 224 33 386 213
Fragments arranged by size:
386 244 224 214 213 33
AvrII C'CTAG_G
Cuts at: 0 486 1314
Size: 486 828
BanI G'GyrC_C
Cuts at: 0 55 719 816 1237 1291 1314
Size: 55 664 97 421 54 23
Fragments arranged by size:
664 421 97 55 54 23
BanII G_rGCy'C
Cuts at: 0 119 198 648 700 918 1051 1108 1314
Size: 119 79 450 52 218 133 57 206
Fragments arranged by size:
450 218 206 133 119 79 57 52
BbeI G_GCGC'C
Cuts at: 0 1295 1314
Size: 1295 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 20 1251 1263 1314
Size: 20 1231 12 51
Fragments arranged by size:
1231 51 20 12
BbvCI CC'TCA_GC
Cuts at: 0 1018 1314
Size: 1018 296
BclI T'GATC_A
Cuts at: 0 395 1314
Size: 395 919
BglI GCCn_nnn'nGGC
Cuts at: 0 167 1314
Size: 167 1147
Bme1580I G_kGCm'C
Cuts at: 0 66 119 198 293 453 648 722 729
Size: 66 53 79 95 160 195 74 7
Cuts at: 729 821 836 918 1108 1314
Size: 92 15 82 190 206
Fragments arranged by size:
206 195 190 160 95 92 82 79
74 66 53 15 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 54 996 1314
Size: 54 942 318
Fragments arranged by size:
942 318 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 396 793 844 1314
Size: 58 338 397 51 470
Fragments arranged by size:
470 397 338 58 51
Bpu10I CC'TnA_GC
Cuts at: 0 143 1018 1314
Size: 143 875 296
Fragments arranged by size:
875 296 143
BsaAI yAC'GTr
Cuts at: 0 516 1314
Size: 516 798
BsaHI Gr'CG_yC
Cuts at: 0 1292 1314
Size: 1292 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 213 1201 1314
Size: 46 167 988 113
Fragments arranged by size:
988 167 113 46
BseYI C'CCAG_C
Cuts at: 0 134 157 171 780 991 1114 1287 1314
Size: 134 23 14 609 211 123 173 27
Fragments arranged by size:
609 211 173 134 123 27 23 14
BsiHKAI G_wGCw'C
Cuts at: 0 293 836 958 1051 1314
Size: 293 543 122 93 263
Fragments arranged by size:
543 293 263 122 93
BsmBI CGTCTCn'nnnn_
Cuts at: 0 900 1314
Size: 900 414
Bsp1286I G_dGCh'C
Cuts at: 0 66 119 198 293 453 648 700 722
Size: 66 53 79 95 160 195 52 22
Cuts at: 722 729 821 836 918 958 1051 1108 1314
Size: 7 92 15 82 40 93 57 206
Fragments arranged by size:
206 195 160 95 93 92 82 79
66 57 53 52 40 22 15 7
BspHI T'CATG_A
Cuts at: 0 980 1314
Size: 980 334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1292 1314
Size: 1292 22
BsrDI GCAATG_nn'
Cuts at: 0 431 1314
Size: 431 883
BstAPI GCAn_nnn'nTGC
Cuts at: 0 840 1314
Size: 840 474
BstXI CCAn_nnnn'nTGG
Cuts at: 0 179 1122 1314
Size: 179 943 192
Fragments arranged by size:
943 192 179
BstYI r'GATC_y
Cuts at: 0 527 1314
Size: 527 787
BtsI GCAGTG_nn'
Cuts at: 0 226 451 1314
Size: 226 225 863
Fragments arranged by size:
863 226 225
EaeI y'GGCC_r
Cuts at: 0 317 968 1314
Size: 317 651 346
Fragments arranged by size:
651 346 317
EarI CTCTTCn'nnn_
Cuts at: 0 688 1255 1314
Size: 688 567 59
Fragments arranged by size:
688 567 59
EcoICRI GAG'CTC
Cuts at: 0 1049 1314
Size: 1049 265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 396 793 844 1314
Size: 58 338 397 51 470
Fragments arranged by size:
470 397 338 58 51
EcoO109I rG'GnC_Cy
Cuts at: 0 48 91 115 116 644 915 1314
Size: 48 43 24 1 528 271 399
Fragments arranged by size:
528 399 271 48 43 24 1
HaeII r_GCGC'y
Cuts at: 0 411 741 1194 1295 1314
Size: 411 330 453 101 19
Fragments arranged by size:
453 411 330 101 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 987 1019 1314
Size: 987 32 295
Fragments arranged by size:
987 295 32
KasI G'GCGC_C
Cuts at: 0 1291 1314
Size: 1291 23
MscI TGG'CCA
Cuts at: 0 970 1314
Size: 970 344
MslI CAynn'nnrTG
Cuts at: 0 296 334 751 757 907 1314
Size: 296 38 417 6 150 407
Fragments arranged by size:
417 407 296 150 38 6
MspA1I CmG'CkG
Cuts at: 0 237 447 471 554 578 780 804 1092
Size: 237 210 24 83 24 202 24 288
Cuts at: 1092 1314
Size: 222
Fragments arranged by size:
288 237 222 210 202 83 24 24
24
NarI GG'CG_CC
Cuts at: 0 1292 1314
Size: 1292 22
NspI r_CATG'y
Cuts at: 0 333 756 912 1314
Size: 333 423 156 402
Fragments arranged by size:
423 402 333 156
PasI CC'CwG_GG
Cuts at: 0 95 1314
Size: 95 1219
PciI A'CATG_T
Cuts at: 0 752 1314
Size: 752 562
PflMI CCAn_nnn'nTGG
Cuts at: 0 76 384 1314
Size: 76 308 930
Fragments arranged by size:
930 308 76
PpuMI rG'GwC_Cy
Cuts at: 0 48 91 1314
Size: 48 43 1223
Fragments arranged by size:
1223 48 43
PspOMI G'GGCC_C
Cuts at: 0 115 194 644 914 1104 1314
Size: 115 79 450 270 190 210
Fragments arranged by size:
450 270 210 190 115 79
PstI C_TGCA'G
Cuts at: 0 233 671 803 1314
Size: 233 438 132 511
Fragments arranged by size:
511 438 233 132
PvuII CAG'CTG
Cuts at: 0 237 578 780 804 1314
Size: 237 341 202 24 510
Fragments arranged by size:
510 341 237 202 24
SacI G_AGCT'C
Cuts at: 0 1051 1314
Size: 1051 263
SanDI GG'GwC_CC
Cuts at: 0 48 91 1314
Size: 48 43 1223
Fragments arranged by size:
1223 48 43
SapI GCTCTTCn'nnn_
Cuts at: 0 688 1255 1314
Size: 688 567 59
Fragments arranged by size:
688 567 59
SfcI C'TryA_G
Cuts at: 0 229 667 799 1314
Size: 229 438 132 515
Fragments arranged by size:
515 438 229 132
SfoI GGC'GCC
Cuts at: 0 1293 1314
Size: 1293 21
SmaI CCC'GGG
Cuts at: 0 460 717 1314
Size: 460 257 597
Fragments arranged by size:
597 460 257
StuI AGG'CCT
Cuts at: 0 208 485 1314
Size: 208 277 829
Fragments arranged by size:
829 277 208
StyI C'CwwG_G
Cuts at: 0 209 486 1034 1314
Size: 209 277 548 280
Fragments arranged by size:
548 280 277 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 594 651 1206 1314
Size: 594 57 555 108
Fragments arranged by size:
594 555 108 57
TatI w'GTAC_w
Cuts at: 0 686 1314
Size: 686 628
XmaI C'CCGG_G
Cuts at: 0 458 715 1314
Size: 458 257 599
Fragments arranged by size:
599 458 257
XmnI GAAnn'nnTTC
Cuts at: 0 1270 1314
Size: 1270 44
Enzymes that do cut:
AccI AclI AfeI AflIII AlfI AlwNI ApaI ApaLI
AvaI AvrII BanI BanII BbeI BbsI BbvCI BclI
BglI Bme1580I BmrI BpmI Bpu10I BsaAI BsaHI BseRI
BseYI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BstAPI
BstXI BstYI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I
HaeII Hin4I KasI MscI MslI MspA1I NarI NspI
PasI PciI PflMI PpuMI PspOMI PstI PvuII SacI
SanDI SapI SfcI SfoI SmaI StuI StyI TaqII
TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AcuI AflII AgeI AhdI AleI
AloI ApoI AscI AseI AsiSI BaeI BamHI BcgI
BciVI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI
BsaI BsaBI BsaWI BsaXI BsgI BsiEI BsiWI BsmI
BspEI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI
ZraI