(Circular) (Six-base) MAPSORT of: pFN21AE6393.seq Check: 9773 from: 1 to: 6019 pFN21AE6393 6019 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3829 278 Size: 53 83 186 936 2293 2468 Fragments arranged by size: 2468 2293 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2499 2949 3362 1937 Size: 562 450 413 4594 Fragments arranged by size: 4594 562 450 413 Acc65I G'GTAC_C Cuts at: 3340 3340 Size: 6019 AclI AA'CG_TT Cuts at: 1563 2979 4146 4519 1563 Size: 1416 1167 373 3063 Fragments arranged by size: 3063 1416 1167 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4082 5466 5734 1632 Size: 2450 1384 268 1917 Fragments arranged by size: 2450 1917 1384 268 AfeI AGC'GCT Cuts at: 2414 2414 Size: 6019 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5822 AflIII A'CryG_T Cuts at: 1203 2757 4918 5895 1203 Size: 1554 2161 977 1327 Fragments arranged by size: 2161 1554 1327 977 AgeI A'CCGG_T Cuts at: 4850 4850 Size: 6019 AhdI GACnn_n'nnGTC Cuts at: 4748 4748 Size: 6019 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6019 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2876 3266 1184 Size: 1692 390 3937 Fragments arranged by size: 3937 1692 390 AlwNI CAG_nnn'CTG Cuts at: 2081 2242 2389 3762 5334 2081 Size: 161 147 1373 1572 2766 Fragments arranged by size: 2766 1572 1373 161 147 ApaI G_GGCC'C Cuts at: 2124 2203 2653 2923 3113 2124 Size: 79 450 270 190 5030 Fragments arranged by size: 5030 450 270 190 79 ApaLI G'TGCA_C Cuts at: 2294 2837 4076 5232 2294 Size: 543 1239 1156 3081 Fragments arranged by size: 3081 1239 1156 543 ApoI r'AATT_y Cuts at: 1493 3328 3554 3706 5815 1493 Size: 1835 226 152 2109 1697 Fragments arranged by size: 2109 1835 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4573 160 Size: 872 3541 1606 Fragments arranged by size: 3541 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6019 AvaI C'yCGr_G Cuts at: 1870 1943 2249 2463 2687 2720 3106 3344 1870 Size: 73 306 214 224 33 386 238 4545 Fragments arranged by size: 4545 386 306 238 224 214 73 33 AvrII C'CTAG_G Cuts at: 2491 2491 Size: 6019 BamHI G'GATC_C Cuts at: 3349 3716 3349 Size: 367 5652 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2724 2821 3242 3296 Size: 325 217 627 273 664 97 421 54 Cuts at: 3296 3340 4795 618 Size: 44 1455 1842 Fragments arranged by size: 1842 1455 664 627 421 325 273 217 97 54 44 BanII G_rGCy'C Cuts at: 1391 2124 2203 2653 2705 2923 3056 3113 3338 Size: 733 79 450 52 218 133 57 225 Cuts at: 3338 1391 Size: 4072 Fragments arranged by size: 4072 733 450 225 218 133 79 57 52 BbeI G_GCGC'C Cuts at: 3300 3300 Size: 6019 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2025 3256 3268 928 Size: 971 126 1231 12 3679 Fragments arranged by size: 3679 1231 971 126 12 BbvCI CC'TCA_GC Cuts at: 3023 3023 Size: 6019 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4210 4244 1903 Size: 34 2273 34 3678 Fragments arranged by size: 3678 2273 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3911 5121 3911 Size: 1210 4809 BclI T'GATC_A Cuts at: 1548 2400 1548 Size: 852 5167 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2172 4630 136 Size: 107 122 71 631 605 500 2458 1525 Fragments arranged by size: 2458 1525 631 605 500 122 107 71 BglII A'GATC_T Cuts at: 6014 6014 Size: 6019 BlpI GC'TnA_GC Cuts at: 3436 3436 Size: 6019 Bme1580I G_kGCm'C Cuts at: 1790 2071 2124 2203 2298 2458 2653 2727 2734 Size: 281 53 79 95 160 195 74 7 Cuts at: 2734 2826 2841 2923 3113 4080 5236 1790 Size: 92 15 82 190 967 1156 2573 Fragments arranged by size: 2573 1156 967 281 195 190 160 95 92 82 79 74 53 15 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6019 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2059 3001 3750 Size: 416 53 83 399 24 642 942 749 Cuts at: 3750 4708 442 Size: 958 1753 Fragments arranged by size: 1753 958 942 749 642 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6019 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 2401 2798 2849 4679 1239 Size: 824 338 397 51 1830 2579 Fragments arranged by size: 2579 1830 824 397 338 51 Bpu10I CC'TnA_GC Cuts at: 2148 3023 2148 Size: 875 5144 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4144 5009 5307 4144 Size: 865 298 4856 Fragments arranged by size: 4856 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4682 882 Size: 617 3183 2219 Fragments arranged by size: 3183 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2521 3810 493 Size: 711 1317 1289 2702 Fragments arranged by size: 2702 1317 1289 711 BsaBI GATnn'nnATC Cuts at: 6013 6013 Size: 6019 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3297 3826 4208 4843 Size: 53 83 186 936 1764 529 382 635 Cuts at: 4843 275 Size: 1451 Fragments arranged by size: 1764 1451 936 635 529 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4452 4850 5124 5271 5682 4452 Size: 398 274 147 411 4789 Fragments arranged by size: 4789 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5794 Fragments arranged by size: 5794 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2218 3206 1186 Size: 865 167 988 3999 Fragments arranged by size: 3999 988 865 167 BseYI C'CCAG_C Cuts at: 2139 2162 2176 2785 2996 3119 3292 5222 5718 Size: 23 14 609 211 123 173 1930 496 Cuts at: 5718 2139 Size: 2440 Fragments arranged by size: 2440 1930 609 496 211 173 123 23 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5446 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3469 4230 5258 1540 Size: 275 186 1468 761 1028 2301 Fragments arranged by size: 2301 1468 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2298 2841 2963 3056 4080 4165 5236 2298 Size: 543 122 93 1024 85 1071 3081 Fragments arranged by size: 3081 1071 1024 543 122 93 85 BsmI GAATG_Cn' Cuts at: 3530 3623 3530 Size: 93 5926 BsmBI CGTCTCn'nnnn_ Cuts at: 2905 2905 Size: 6019 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2124 2203 2298 2458 2653 2705 Size: 399 281 53 79 95 160 195 52 Cuts at: 2705 2727 2734 2826 2841 2923 2963 3056 3113 Size: 22 7 92 15 82 40 93 57 Cuts at: 3113 3338 4080 4165 5236 1391 Size: 225 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 281 225 195 160 95 93 92 85 82 79 57 53 52 40 22 15 7 BspEI T'CCGG_A Cuts at: 5682 5682 Size: 6019 BspHI T'CATG_A Cuts at: 2985 3908 5638 2985 Size: 923 1730 3366 Fragments arranged by size: 3366 1730 923 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3297 3374 844 Size: 2453 77 3489 Fragments arranged by size: 3489 2453 77 BsrBI CCG'CTC Cuts at: 1394 1823 3906 1394 Size: 429 2083 3507 Fragments arranged by size: 3507 2083 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2436 4514 4688 65 Size: 1183 1188 2078 174 1396 Fragments arranged by size: 2078 1396 1188 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4663 4850 1700 Size: 110 2853 187 2869 Fragments arranged by size: 2869 2853 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6019 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6019 BssSI C'ACGA_G Cuts at: 4079 5091 4079 Size: 1012 5007 BstAPI GCAn_nnn'nTGC Cuts at: 794 2845 794 Size: 2051 3968 BstBI TT'CG_AA Cuts at: 4823 4823 Size: 6019 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6019 BstXI CCAn_nnnn'nTGG Cuts at: 2184 3127 2184 Size: 943 5076 BstYI r'GATC_y Cuts at: 1972 2532 3349 3716 4101 4118 5570 5656 5668 Size: 560 817 367 385 17 1452 86 12 Cuts at: 5668 6014 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 817 560 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4528 Fragments arranged by size: 4528 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5280 Fragments arranged by size: 5280 683 56 BtsI GCAGTG_nn' Cuts at: 2231 2456 3535 4329 4349 2231 Size: 225 1079 794 20 3901 Fragments arranged by size: 3901 1079 794 225 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6019 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5984 DraI TTT'AAA Cuts at: 3323 3323 Size: 6019 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5026 809 Size: 507 3710 1802 Fragments arranged by size: 3710 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2322 2973 3466 Size: 54 1425 50 275 18 492 651 493 Cuts at: 3466 4355 5984 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 651 493 492 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3466 1537 Size: 275 1654 4090 Fragments arranged by size: 4090 1654 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2693 3260 3949 1353 Size: 1340 567 689 3423 Fragments arranged by size: 3423 1340 689 567 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4606 4980 5126 387 Size: 1064 138 3017 374 146 1280 Fragments arranged by size: 3017 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3054 3054 Size: 6019 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2401 2798 2849 4082 4679 5466 Size: 393 431 338 397 51 1233 597 787 Cuts at: 5466 5734 1239 Size: 268 1524 Fragments arranged by size: 1524 1233 787 597 431 397 393 338 268 51 EcoO109I rG'GnC_Cy Cuts at: 2053 2096 2120 2121 2649 2920 2053 Size: 43 24 1 528 271 5152 Fragments arranged by size: 5152 528 271 43 24 1 EcoRI G'AATT_C Cuts at: 3328 3706 3328 Size: 378 5641 FspI TGC'GCA Cuts at: 4525 4525 Size: 6019 HaeII r_GCGC'y Cuts at: 1678 2416 2746 3199 3300 5166 1678 Size: 738 330 453 101 1866 2531 Fragments arranged by size: 2531 1866 738 453 330 101 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2992 3024 4660 4692 4734 Size: 32 135 32 1420 32 1636 32 42 Cuts at: 4734 4766 1373 Size: 32 2626 Fragments arranged by size: 2626 1636 1420 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3363 3609 669 Size: 988 281 1425 246 3079 Fragments arranged by size: 3079 1425 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6019 HpaI GTT'AAC Cuts at: 3609 3609 Size: 6019 KasI G'GCGC_C Cuts at: 3296 3296 Size: 6019 KpnI G_GTAC'C Cuts at: 3344 3344 Size: 6019 MfeI C'AATT_G Cuts at: 3618 3618 Size: 6019 MluI A'CGCG_T Cuts at: 5895 5895 Size: 6019 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5133 5317 1305 Size: 3828 184 2007 Fragments arranged by size: 3828 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2975 10 Size: 54 1425 343 1143 3054 Fragments arranged by size: 3054 1425 1143 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2301 2339 2756 2762 2912 3977 4336 Size: 648 1135 38 417 6 150 1065 359 Cuts at: 4336 4495 518 Size: 159 2042 Fragments arranged by size: 2042 1135 1065 648 417 359 159 150 38 6 MspA1I CmG'CkG Cuts at: 1606 2242 2452 2476 2559 2583 2785 2809 3097 Size: 636 210 24 83 24 202 24 288 Cuts at: 3097 3435 4112 5260 5505 1606 Size: 338 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 636 338 288 245 210 202 83 24 24 24 NarI GG'CG_CC Cuts at: 3297 3297 Size: 6019 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4528 Fragments arranged by size: 4528 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6019 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6019 NotI GC'GGCC_GC Cuts at: 3466 3466 Size: 6019 NspI r_CATG'y Cuts at: 1132 1229 2338 2761 2917 3377 4922 1132 Size: 97 1109 423 156 460 1545 2229 Fragments arranged by size: 2229 1545 1109 460 423 156 97 PasI CC'CwG_GG Cuts at: 1348 2100 1348 Size: 752 5267 PciI A'CATG_T Cuts at: 2757 4918 2757 Size: 2161 3858 PflMI CCAn_nnn'nTGG Cuts at: 1108 2081 2389 1108 Size: 973 308 4738 Fragments arranged by size: 4738 973 308 PmeI GTTT'AAAC Cuts at: 3323 3323 Size: 6019 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4088 4120 5601 5633 4088 Size: 32 1481 32 4474 Fragments arranged by size: 4474 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2053 2096 2053 Size: 43 5976 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6019 PsiI TTA'TAA Cuts at: 3589 3589 Size: 6019 PspOMI G'GGCC_C Cuts at: 2120 2199 2649 2919 3109 2120 Size: 79 450 270 190 5030 Fragments arranged by size: 5030 450 270 190 79 PstI C_TGCA'G Cuts at: 830 2238 2676 2808 3371 830 Size: 1408 438 132 563 3478 Fragments arranged by size: 3478 1408 563 438 132 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6019 PvuII CAG'CTG Cuts at: 2242 2583 2785 2809 2242 Size: 341 202 24 5452 Fragments arranged by size: 5452 341 202 24 SacI G_AGCT'C Cuts at: 3056 3056 Size: 6019 SalI G'TCGA_C Cuts at: 1936 3361 1936 Size: 1425 4594 SanDI GG'GwC_CC Cuts at: 2053 2096 2053 Size: 43 5976 SapI GCTCTTCn'nnn_ Cuts at: 2693 3260 2693 Size: 567 5452 SbfI CC_TGCA'GG Cuts at: 3371 3371 Size: 6019 ScaI AGT'ACT Cuts at: 4267 5979 4267 Size: 1712 4307 SfcI C'TryA_G Cuts at: 826 1045 2234 2672 2804 3367 4502 5183 5374 Size: 219 1189 438 132 563 1135 681 191 Cuts at: 5374 826 Size: 1471 Fragments arranged by size: 1471 1189 1135 681 563 438 219 191 132 SfoI GGC'GCC Cuts at: 3298 3298 Size: 6019 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6019 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6019 SmaI CCC'GGG Cuts at: 1872 2465 2722 3346 1872 Size: 593 257 624 4545 Fragments arranged by size: 4545 624 593 257 SmlI C'TyrA_G Cuts at: 820 1017 1943 4123 5024 5286 820 Size: 197 926 2180 901 262 1553 Fragments arranged by size: 2180 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3810 493 Size: 3317 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6019 SphI G_CATG'C Cuts at: 1132 3377 1132 Size: 2245 3774 SspI AAT'ATT Cuts at: 5 52 3943 5 Size: 47 3891 2081 Fragments arranged by size: 3891 2081 47 StuI AGG'CCT Cuts at: 1517 2213 2490 1517 Size: 696 277 5046 Fragments arranged by size: 5046 696 277 StyI C'CwwG_G Cuts at: 513 1065 2004 2214 2491 3039 3458 513 Size: 552 939 210 277 548 419 3074 Fragments arranged by size: 3074 939 552 548 419 277 210 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2599 2656 3211 4046 4063 4216 4401 Size: 1255 1019 57 555 835 17 153 185 Cuts at: 4401 4846 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1019 835 555 445 185 153 57 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2691 4265 5977 Size: 274 80 33 51 1443 714 1574 1712 Cuts at: 5977 96 Size: 138 Fragments arranged by size: 1712 1574 1443 714 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5979 6011 5979 Size: 32 5987 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6019 XbaI T'CTAG_A Cuts at: 3355 3355 Size: 6019 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6019 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6019 XmaI C'CCGG_G Cuts at: 1870 2463 2720 3344 1870 Size: 593 257 624 4545 Fragments arranged by size: 4545 624 593 257 XmnI GAAnn'nnTTC Cuts at: 3275 4148 3275 Size: 873 5146 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3827 276 Size: 53 83 186 936 2293 2468 Fragments arranged by size: 2468 2293 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI