Restriction map for
pFN21AE6393
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccIII AcsI AflII
AgeI AhdI AocI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII BamHI BbrPI BcgI BciVI
BfrI BglII BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HgaI HincII HindII HindIII
HinfI HpaI KpnI KspI MamI
MfeI MluI MroI MseI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AclI 973 AACGTT AA^CGTT
AcyI 1291 GGCGCC G[GA]^CG[CT]C
AflIII 752 ACATGT A^C[GA][CT]GT
Aor51HI 407 AGCGCT AGC^GCT
Asp700I 1266 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 486 CCTAGG C^CTAGG
BalI 968 TGGCCA TGG^CCA
BbeI 1291 GGCGCC GGCGC^C
BbvCI 1017 GCTGAGG CCTCAGC(-5/-2)
BclI 395 TGATCA T^GATCA
BfaI 487 CTAG C^TAG
BglI 161 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 486 CCTAGG C^CTAGG
BsaAI 514 TACGTG [CT]AC^GT[GA]
BsaHI 1291 GGCGCC G[GA]^CG[CT]C
BsmAI 895 GTCTC GTCTC(1/5)
BsmBI 894 CGTCTC CGTCTC(1/5)
BspHI 980 TCATGA T^CATGA
BspLU11I 752 ACATGT A^CATGT
BspMI 1283 ACCTGC ACCTGC(4/8)
BsrDI 432 CATTGC GCAATG(2/0)
BstAPI 834 GCACGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 527 AGATCC [GA]^GATC[CT]
Eco47III 407 AGCGCT AGC^GCT
FbaI 395 TGATCA T^GATCA
Hin1I 1291 GGCGCC G[GA]^CG[CT]C
KasI 1291 GGCGCC G^GCGCC
MaeI 487 CTAG C^TAG
MflI 527 AGATCC [GA]^GATC[CT]
MluNI 968 TGGCCA TGG^CCA
MscI 968 TGGCCA TGG^CCA
NarI 1291 GGCGCC GG^CGCC
Psp1406I 973 AACGTT AA^CGTT
RcaI 980 TCATGA T^CATGA
SacI 1047 GAGCTC GAGCT^C
SfoI 1291 GGCGCC GGC^GCC
XhoII 527 AGATCC [GA]^GATC[CT]
XmnI 1266 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 493 GTCTAC GT^[AC][GT]AC
943 GTCTAC
AccII 730 CGCG CG^CG
1140 CGCG
Alw44I 289 GTGCAC G^TGCAC
832 GTGCAC
AlwI 528 GATCC GGATC(4/5)
80 GGATC
ApaLI 289 GTGCAC G^TGCAC
832 GTGCAC
BstUI 730 CGCG CG^CG
1140 CGCG
BstXI 172 CCAGCCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1115 CCAGCTTCATGG
EaeI 317 CGGCCA [CT]^GGCC[GA]
968 TGGCCA
EarI 1249 CTCTTC CTCTTC(1/4)
693 GAAGAG
Ksp632I 1249 CTCTTC CTCTTC(1/4)
693 GAAGAG
MvnI 730 CGCG CG^CG
1140 CGCG
PflMI 70 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
378 CCAGGAGCTGG
PpuMI 47 GGGACCC [GA]G^G[AT]CC[CT]
90 GGGTCCC
SapI 693 GAAGAGC GCTCTTC(1/4)
1248 GCTCTTC
SfaNI 151 GATGC GCATC(5/9)
848 GCATC
SmaI 458 CCCGGG CCC^GGG
715 CCCGGG
StuI 206 AGGCCT AGG^CCT
483 AGGCCT
Tsp45I 569 GTCAC ^GT[GC]AC
607 GTCAC
Van91I 70 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
378 CCAGGAGCTGG
XmaCI 458 CCCGGG C^CCGGG
715 CCCGGG
XmaI 458 CCCGGG C^CCGGG
715 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TseI TspRI