AQP12B ORF clone pFN21AE6288

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE6288

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Clone info.

Species

human

Clone Name

pFN21AE6288 Sequence (with vector) Restriction Map

Product ID

FHC30940

ORF Sequence

DNA sequence (921 bp)
Protein sequence (307 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6288

Symbol

AQP12B

Alias : INSSA3

Description

aquaporin 12B

Original Clone ID

oc96288

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 921 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 307 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS46560.1 8e-128 100.0 AQP12B CCDS8251.1 3.7e-19 30.6 AQP11 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR023265 1 10 PR02025 Aquaporin 12 IPR023265 18 28 PR02025 Aquaporin 12 IPR023265 32 41 PR02025 Aquaporin 12 IPR023265 54 67 PR02025 Aquaporin 12 IPR000425 73 97 PR00783 Major intrinsic protein IPR023265 99 111 PR02025 Aquaporin 12 IPR023265 126 139 PR02025 Aquaporin 12 IPR023265 143 152 PR02025 Aquaporin 12 IPR023265 153 162 PR02025 Aquaporin 12 IPR023265 180 190 PR02025 Aquaporin 12 IPR000425 193 215 PR00783 Major intrinsic protein IPR023265 219 231 PR02025 Aquaporin 12 IPR000425 233 253 PR00783 Major intrinsic protein IPR023265 250 262 PR02025 Aquaporin 12 IPR023265 263 276 PR02025 Aquaporin 12 HMMPfam IPR000425 76 250 PF00230 Major intrinsic protein
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 1 MAGLNVSLSFFFATFTLCEAA 21 SECONDARY 21 2 66 DFGPDLLLTLLFLLFLAHGVTLD 88 PRIMARY 23 3 116 AAQGLGMQAACTLTRLCWAWELS 138 SECONDARY 23 4 161 HGALVEAACAFCFHLTLLHLRHS 183 SECONDARY 23 5 196 VTVTAYTAGPFTSAFFNPALAAS 218 SECONDARY 23 6 233 QVYWLGPLTGMVLAVLLHQGRLP 255 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_945349 295 609789 95.1 100.0 NP_766627 271 609914 30.6 81.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6288 Download

>KIEE6288  921 bp
ATGGCAGGTCTTAACGTGTCCCTCTCCTTCTTCTTTGCCACCTTCACCCTCTGTGAGGCA
GCCAGGCGGGCCTCCAAGGCCCTGCTCCCAGTGGGCGCCTATGAAGTCTTCGCCCGGGAG
GCGGTGGGCGCGGTGCAGCTCGGGGCCTGCTTCCTGGAGATGAGGACGCTGGTCGAGCTC
GGGCCCTGGGCTGGGGACTTTGGGCCTGACCTGCTGCTCACCCTGCTCTTCCTGCTCTTC
CTGGCGCACGGGGTCACCTTGGACGGGGCCTCGGCCAACCCCACCGTGTCCCTGCAGGAG
TTCCTCATGGCCGAGGAGTCTCTGCCTGGCACGCTGCTGAAGCTGGCGGCACAGGGGCTG
GGCATGCAGGCCGCCTGCACCCTGACGCGCCTCTGCTGGGCCTGGGAGCTCAGTGACCTG
CACCTGCTGCAGAGCCTCATGGCCCAGAGCTGCAGCTCGGCCCTGCGCACATCCGTGCCC
CACGGGGCGCTTGTGGAGGCCGCCTGCGCCTTTTGTTTCCATCTGACCCTCCTGCACCTG
CGGCACAGTCCTCCCGCCTACAGCGGGCCCGCTGTGGCTCTGTTGGTCACCGTCACGGCC
TACACGGCCGGGCCCTTCACGTCTGCCTTCTTCAACCCTGCCCTGGCCGCCTCTGTGACC
TTTGCCTGCTCGGGACACACCTTACTGGAGTACGTGCAGGTGTACTGGCTGGGCCCTCTG
ACAGGGATGGTCCTGGCTGTGCTGCTGCACCAGGGCCGCCTTCCCCACCTTTTCCAGAGG
AACCTGTTCTACGGCCAGAAGAACAAGTACCGAGCACCCCGAGGGAAGCCGGCCCCGGCC
TCAGGGGACACCCAGACCCCTGCAAAGGGGTCCAGTGTCCGGGAGCCTGGGCGCAGTGGT
GTTGAGGGGCCACATTCCAGC

ORF Protein

Cloned ORF protein sequence for pFN21AE6288 Download

>KIEE6288  307 aa
MAGLNVSLSFFFATFTLCEAARRASKALLPVGAYEVFAREAVGAVQLGACFLEMRTLVEL
GPWAGDFGPDLLLTLLFLLFLAHGVTLDGASANPTVSLQEFLMAEESLPGTLLKLAAQGL
GMQAACTLTRLCWAWELSDLHLLQSLMAQSCSSALRTSVPHGALVEAACAFCFHLTLLHL
RHSPPAYSGPAVALLVTVTAYTAGPFTSAFFNPALAASVTFACSGHTLLEYVQVYWLGPL
TGMVLAVLLHQGRLPHLFQRNLFYGQKNKYRAPRGKPAPASGDTQTPAKGSSVREPGRSG
VEGPHSS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6288 Download

>pFN21AE6288  5626 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAGGTCTTAACGTGTCCCTCTCCTTCTTCTT
TGCCACCTTCACCCTCTGTGAGGCAGCCAGGCGGGCCTCCAAGGCCCTGCTCCCAGTGGG
CGCCTATGAAGTCTTCGCCCGGGAGGCGGTGGGCGCGGTGCAGCTCGGGGCCTGCTTCCT
GGAGATGAGGACGCTGGTCGAGCTCGGGCCCTGGGCTGGGGACTTTGGGCCTGACCTGCT
GCTCACCCTGCTCTTCCTGCTCTTCCTGGCGCACGGGGTCACCTTGGACGGGGCCTCGGC
CAACCCCACCGTGTCCCTGCAGGAGTTCCTCATGGCCGAGGAGTCTCTGCCTGGCACGCT
GCTGAAGCTGGCGGCACAGGGGCTGGGCATGCAGGCCGCCTGCACCCTGACGCGCCTCTG
CTGGGCCTGGGAGCTCAGTGACCTGCACCTGCTGCAGAGCCTCATGGCCCAGAGCTGCAG
CTCGGCCCTGCGCACATCCGTGCCCCACGGGGCGCTTGTGGAGGCCGCCTGCGCCTTTTG
TTTCCATCTGACCCTCCTGCACCTGCGGCACAGTCCTCCCGCCTACAGCGGGCCCGCTGT
GGCTCTGTTGGTCACCGTCACGGCCTACACGGCCGGGCCCTTCACGTCTGCCTTCTTCAA
CCCTGCCCTGGCCGCCTCTGTGACCTTTGCCTGCTCGGGACACACCTTACTGGAGTACGT
GCAGGTGTACTGGCTGGGCCCTCTGACAGGGATGGTCCTGGCTGTGCTGCTGCACCAGGG
CCGCCTTCCCCACCTTTTCCAGAGGAACCTGTTCTACGGCCAGAAGAACAAGTACCGAGC
ACCCCGAGGGAAGCCGGCCCCGGCCTCAGGGGACACCCAGACCCCTGCAAAGGGGTCCAG
TGTCCGGGAGCCTGGGCGCAGTGGTGTTGAGGGGCCACATTCCAGCGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT