Restriction map for
pFN21AE6288
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstXI BstYI
BstZ17I CelII ClaI CpoI DpnII
DraI DraIII DrdI Eam1105I Eco47III
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MunI NcoI NdeI NdeII NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI Sau3AI ScaI SexAI SfaNI
SfuI SgrAI SmlI SnaBI SpeI
SspBI SspI StuI SwaI TfiI
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XhoII
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 94 GGCGCC G[GA]^CG[CT]C
AflIII 14 ACGTGT A^C[GA][CT]GT
AlwNI 352 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 839 CCTCAGG CC^T[ACGT]AGG
Asp700I 780 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 464 TGCGCA TGC^GCA
BanI 94 GGCGCC G^G[CT][GA]CC
BbeI 94 GGCGCC GGCGC^C
BbsI 106 GTCTTC GAAGAC(2/6)
BpuAI 106 GTCTTC GAAGAC(2/6)
BsaAI 691 TACGTG [CT]AC^GT[GA]
BsaHI 94 GGCGCC G[GA]^CG[CT]C
BseRI 313 GAGGAG GAGGAG(10/8)
BsiEI 605 CGGCCG CG[GA][CT]^CG
BsmAI 318 GTCTC GTCTC(1/5)
BsrFI 828 GCCGGC [GA]^CCGG[CT]
BstAPI 420 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 839 CCTCAGG CC^T[ACGT]AGG
Cfr10I 828 GCCGGC [GA]^CCGG[CT]
DsaI 480 CCACGG C^C[GA][CT]GG
EagI 605 CGGCCG C^GGCCG
EclXI 605 CGGCCG C^GGCCG
Eco52I 605 CGGCCG C^GGCCG
Eco81I 839 CCTCAGG CC^T[ACGT]AGG
FspI 464 TGCGCA TGC^GCA
Hin1I 94 GGCGCC G[GA]^CG[CT]C
HinfI 316 GAGTC G^A[ACGT]TC
KasI 94 GGCGCC G^GCGCC
MseI 11 TTAA T^TAA
MslI 468 CACATCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 828 GCCGGC GCC^GGC
NarI 94 GGCGCC GG^CGCC
NgoMIV 828 GCCGGC G^CCGGC
NspI 362 GCATGC [GA]CATG^[CT]
PleI 316 GAGTC GAGTC(4/5)
PshAI 165 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 291 CCTGCAGG CCTGCA^GG
SfoI 94 GGCGCC GGC^GCC
SmaI 113 CCCGGG CCC^GGG
SphI 362 GCATGC GCATG^C
Sse8387I 291 CCTGCAGG CCTGCA^GG
TaqI 173 TCGA T^CGA
Tru9I 11 TTAA T^TAA
TthHB8I 173 TCGA T^CGA
XmaCI 113 CCCGGG C^CCGGG
XmaI 113 CCCGGG C^CCGGG
XmnI 780 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 129 CGCG CG^CG
386 CGCG
AvaII 729 GGTCC G^G[AT]CC
869 GGTCC
BpmI 154 CTGGAG CTGGAG(16/14)
685 CTGGAG
BstEII 252 GGTCACC G^GT[ACGT]ACC
585 GGTCACC
BstF5I 470 CATCC GGATG(2/0)
725 GGATG
BstPI 252 GGTCACC G^GT[ACGT]ACC
585 GGTCACC
BstUI 129 CGCG CG^CG
386 CGCG
DdeI 409 CTCAG C^T[ACGT]AG
840 CTCAG
EarI 226 CTCTTC CTCTTC(1/4)
235 CTCTTC
EcoNI 48 CCTCTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
715 CCTCTGACAGG
EcoO65I 252 GGTCACC G^GT[ACGT]ACC
585 GGTCACC
EcoT14I 74 CCAAGG C^C[AT][AT]GG
257 CCTTGG
FokI 470 CATCC GGATG(9/13)
725 GGATG
HaeII 94 GGCGCC [GA]GCGC^[CT]
486 GGCGCT
HgaI 165 GACGC GACGC(5/10)
384 GACGC
Ksp632I 226 CTCTTC CTCTTC(1/4)
235 CTCTTC
MspA1I 561 CAGCGG C[AC]G^C[GT]G
569 CCGCTG
MvnI 129 CGCG CG^CG
386 CGCG
SacI 175 GAGCTC GAGCT^C
406 GAGCTC
SapI 225 GCTCTTC GCTCTTC(1/4)
234 GCTCTTC
SfiI 69 GGCCTCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
597 GGCCTACACGGCC
StyI 74 CCAAGG C^C[AT][AT]GG
257 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI ApaI AspHI
AvaI BanII BbvI BcnI BglI
BmyI BsaJI BsgI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstNI Cac8I CfoI
Cfr13I DraII EaeI EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MaeIII MboII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV PstI
RsaI Sau96I ScrFI SfcI TseI
Tsp45I TspRI