(Circular) (Six-base) MAPSORT of: pFN21AE6288.seq Check: 7085 from: 1 to: 5626 pFN21AE6288 5626 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2436 2550 2692 2436 Size: 114 142 5370 Fragments arranged by size: 5370 142 114 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3436 278 Size: 53 83 186 936 1900 2468 Fragments arranged by size: 2468 1900 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2969 1937 Size: 1032 4594 Acc65I G'GTAC_C Cuts at: 2947 2947 Size: 5626 AclI AA'CG_TT Cuts at: 1563 3753 4126 1563 Size: 2190 373 3063 Fragments arranged by size: 3063 2190 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2363 3689 5073 5341 1632 Size: 731 1326 1384 268 1917 Fragments arranged by size: 1917 1384 1326 731 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5429 AflIII A'CryG_T Cuts at: 1203 2019 4525 5502 1203 Size: 816 2506 977 1327 Fragments arranged by size: 2506 1327 977 816 AgeI A'CCGG_T Cuts at: 4457 4457 Size: 5626 AhdI GACnn_n'nnGTC Cuts at: 4355 4355 Size: 5626 AleI CACnn'nnGTG Cuts at: 1166 2477 1166 Size: 1311 4315 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2495 1184 Size: 1311 4315 AlwNI CAG_nnn'CTG Cuts at: 2362 3369 4941 2362 Size: 1007 1572 3047 Fragments arranged by size: 3047 1572 1007 ApaI G_GGCC'C Cuts at: 2190 2574 2619 2720 2190 Size: 384 45 101 5096 Fragments arranged by size: 5096 384 101 45 ApaLI G'TGCA_C Cuts at: 3683 4839 3683 Size: 1156 4470 ApoI r'AATT_y Cuts at: 1493 2935 3161 3313 5422 1493 Size: 1442 226 152 2109 1697 Fragments arranged by size: 2109 1697 1442 226 152 AseI AT'TA_AT Cuts at: 160 1032 4180 160 Size: 872 3148 1606 Fragments arranged by size: 3148 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 AvaI C'yCGr_G Cuts at: 1870 1943 2118 2144 2183 2674 2823 2951 1870 Size: 73 175 26 39 491 149 128 4545 Fragments arranged by size: 4545 491 175 149 128 73 39 26 BamHI G'GATC_C Cuts at: 2956 3323 2956 Size: 367 5259 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2099 2947 4402 618 Size: 325 217 627 312 848 1455 1842 Fragments arranged by size: 1842 1455 848 627 325 312 217 BanII G_rGCy'C Cuts at: 1391 2184 2190 2415 2574 2619 2720 2945 1391 Size: 793 6 225 159 45 101 225 4072 Fragments arranged by size: 4072 793 225 225 159 101 45 6 BbeI G_GCGC'C Cuts at: 2103 2103 Size: 5626 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2104 928 Size: 971 205 4450 Fragments arranged by size: 4450 971 205 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3817 3851 1903 Size: 34 1880 34 3678 Fragments arranged by size: 3678 1880 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3518 4728 3518 Size: 1210 4416 BclI T'GATC_A Cuts at: 1548 1548 Size: 5626 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2072 2081 2123 Size: 107 122 71 631 605 400 9 42 Cuts at: 2123 2609 4237 136 Size: 486 1628 1525 Fragments arranged by size: 1628 1525 631 605 486 400 122 107 71 42 9 BglII A'GATC_T Cuts at: 5621 5621 Size: 5626 BlpI GC'TnA_GC Cuts at: 3043 3043 Size: 5626 Bme1580I G_kGCm'C Cuts at: 1790 2190 2484 2574 2619 2720 3687 4843 1790 Size: 400 294 90 45 101 967 1156 2573 Fragments arranged by size: 2573 1156 967 400 294 101 90 45 BmgBI CAC'GTC Cuts at: 1318 2625 1318 Size: 1307 4319 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2087 3357 4315 Size: 416 53 83 399 24 670 1270 958 Cuts at: 4315 442 Size: 1753 Fragments arranged by size: 1753 1270 958 670 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5626 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2180 2711 4286 1239 Size: 941 531 1575 2579 Fragments arranged by size: 2579 1575 941 531 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3751 4616 4914 3751 Size: 865 298 4463 Fragments arranged by size: 4463 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4289 882 Size: 617 2790 2219 Fragments arranged by size: 2790 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2698 3417 493 Size: 711 1494 719 2702 Fragments arranged by size: 2702 1494 719 711 BsaBI GATnn'nnATC Cuts at: 5620 5620 Size: 5626 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2100 3433 3815 4450 Size: 53 83 186 936 567 1333 382 635 Cuts at: 4450 275 Size: 1451 Fragments arranged by size: 1451 1333 936 635 567 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4059 4457 4731 4878 5289 4059 Size: 398 274 147 411 4396 Fragments arranged by size: 4396 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5401 Fragments arranged by size: 5401 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2333 1186 Size: 1147 4479 BseYI C'CCAG_C Cuts at: 2195 2362 2400 2713 4829 5325 2195 Size: 167 38 313 2116 496 2496 Fragments arranged by size: 2496 2116 496 313 167 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2159 2365 2408 2522 2720 2735 1163 Size: 573 423 206 43 114 198 15 4054 Fragments arranged by size: 4054 573 423 206 198 114 43 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2613 3076 3837 4865 1540 Size: 275 186 612 463 761 1028 2301 Fragments arranged by size: 2301 1028 761 612 463 275 186 BsiHKAI G_wGCw'C Cuts at: 2184 2415 2821 3687 3772 4843 2184 Size: 231 406 866 85 1071 2967 Fragments arranged by size: 2967 1071 866 406 231 85 BsmI GAATG_Cn' Cuts at: 3137 3230 3137 Size: 93 5533 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2184 2190 2415 2484 2574 2619 2720 Size: 399 394 6 225 69 90 45 101 Cuts at: 2720 2821 2945 3687 3772 4843 1391 Size: 101 124 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 394 225 124 101 101 90 85 69 45 6 BspEI T'CCGG_A Cuts at: 5289 5289 Size: 5626 BspHI T'CATG_A Cuts at: 3515 5245 3515 Size: 1730 3896 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2000 2223 2430 2436 2550 2692 2981 844 Size: 1156 223 207 6 114 142 289 3489 Fragments arranged by size: 3489 1156 289 223 207 142 114 6 BsrBI CCG'CTC Cuts at: 1394 1823 3513 1394 Size: 429 1690 3507 Fragments arranged by size: 3507 1690 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4121 4295 65 Size: 1183 2873 174 1396 Fragments arranged by size: 2873 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2833 4270 4457 1700 Size: 110 1023 1437 187 2869 Fragments arranged by size: 2869 1437 1023 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5626 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5626 BssSI C'ACGA_G Cuts at: 3686 4698 3686 Size: 1012 4614 BstAPI GCAn_nnn'nTGC Cuts at: 794 2431 794 Size: 1637 3989 BstBI TT'CG_AA Cuts at: 4430 4430 Size: 5626 BstEII G'GTnAC_C Cuts at: 1184 2257 2590 1184 Size: 1073 333 4220 Fragments arranged by size: 4220 1073 333 BstYI r'GATC_y Cuts at: 1972 2956 3323 3708 3725 5177 5263 5275 5621 Size: 984 367 385 17 1452 86 12 346 Cuts at: 5621 1972 Size: 1977 Fragments arranged by size: 1977 1452 984 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2845 2845 Size: 5626 BtgI C'CryG_G Cuts at: 513 1065 2004 2485 513 Size: 552 939 481 3654 Fragments arranged by size: 3654 939 552 481 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4887 Fragments arranged by size: 4887 683 56 BtsI GCAGTG_nn' Cuts at: 2905 3142 3936 3956 2905 Size: 237 794 20 4575 Fragments arranged by size: 4575 794 237 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5626 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5591 DraI TTT'AAA Cuts at: 2930 2930 Size: 5626 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4633 809 Size: 507 3317 1802 Fragments arranged by size: 3317 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2277 2313 2610 Size: 54 1425 50 275 18 447 36 297 Cuts at: 2610 2649 2797 3073 3962 5591 8 Size: 39 148 276 889 1629 43 Fragments arranged by size: 1629 1425 889 447 297 276 275 148 54 50 43 39 36 18 EagI C'GGCC_G Cuts at: 1537 1812 2610 3073 1537 Size: 275 798 463 4090 Fragments arranged by size: 4090 798 463 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2237 2246 3556 1353 Size: 884 9 1310 3423 Fragments arranged by size: 3423 1310 884 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4213 4587 4733 387 Size: 1064 138 2624 374 146 1280 Fragments arranged by size: 2624 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2182 2413 2182 Size: 231 5395 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 2363 2711 3689 4286 5073 5341 Size: 393 548 183 348 978 597 787 268 Cuts at: 5341 1239 Size: 1524 Fragments arranged by size: 1524 978 787 597 548 393 348 268 183 EcoNI CCTnn'n_nnAGG Cuts at: 2057 2724 2057 Size: 667 4959 EcoO109I rG'GnC_Cy Cuts at: 2083 2148 2187 2271 2616 2717 2083 Size: 65 39 84 345 101 4992 Fragments arranged by size: 4992 345 101 84 65 39 EcoRI G'AATT_C Cuts at: 2935 3313 2935 Size: 378 5248 FspI TGC'GCA Cuts at: 2471 4132 2471 Size: 1661 3965 HaeII r_GCGC'y Cuts at: 1678 2103 2495 4773 1678 Size: 425 392 2278 2531 Fragments arranged by size: 2531 2278 425 392 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2258 2290 4267 4299 4341 Size: 32 135 32 686 32 1977 32 42 Cuts at: 4341 4373 1373 Size: 32 2626 Fragments arranged by size: 2626 1977 686 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2970 3216 669 Size: 988 281 1032 246 3079 Fragments arranged by size: 3079 1032 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5626 HpaI GTT'AAC Cuts at: 3216 3216 Size: 5626 KasI G'GCGC_C Cuts at: 2099 2099 Size: 5626 KpnI G_GTAC'C Cuts at: 2951 2951 Size: 5626 MfeI C'AATT_G Cuts at: 3225 3225 Size: 5626 MluI A'CGCG_T Cuts at: 5502 5502 Size: 5626 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4740 4924 1305 Size: 3435 184 2007 Fragments arranged by size: 3435 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3804 Fragments arranged by size: 3804 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2477 3584 3943 4102 518 Size: 648 1311 1107 359 159 2042 Fragments arranged by size: 2042 1311 1107 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2568 2576 3042 3719 4867 5112 1606 Size: 962 8 466 677 1148 245 2120 Fragments arranged by size: 2120 1148 962 677 466 245 8 NaeI GCC'GGC Cuts at: 2835 2835 Size: 5626 NarI GG'CG_CC Cuts at: 2100 2100 Size: 5626 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4135 Fragments arranged by size: 4135 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5626 NgoMIV G'CCGG_C Cuts at: 2833 2833 Size: 5626 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5626 NotI GC'GGCC_GC Cuts at: 3073 3073 Size: 5626 NspI r_CATG'y Cuts at: 1132 1229 2371 2984 4529 1132 Size: 97 1142 613 1545 2229 Fragments arranged by size: 2229 1545 1142 613 97 PasI CC'CwG_GG Cuts at: 1348 2190 1348 Size: 842 4784 PciI A'CATG_T Cuts at: 4525 4525 Size: 5626 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5626 PfoI T'CCnGG_A Cuts at: 2157 2883 2157 Size: 726 4900 PmeI GTTT'AAAC Cuts at: 2930 2930 Size: 5626 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3695 3727 5208 5240 3695 Size: 32 1481 32 4081 Fragments arranged by size: 4081 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2174 1613 Size: 561 5065 PsiI TTA'TAA Cuts at: 3196 3196 Size: 5626 PspOMI G'GGCC_C Cuts at: 2186 2570 2615 2716 2186 Size: 384 45 101 5096 Fragments arranged by size: 5096 384 101 45 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2777 2809 2777 Size: 32 5594 PstI C_TGCA'G Cuts at: 830 2301 2436 2459 2978 830 Size: 1471 135 23 519 3478 Fragments arranged by size: 3478 1471 519 135 23 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5626 SacI G_AGCT'C Cuts at: 2184 2415 2184 Size: 231 5395 SalI G'TCGA_C Cuts at: 1936 2968 1936 Size: 1032 4594 SapI GCTCTTCn'nnn_ Cuts at: 2237 2246 2237 Size: 9 5617 SbfI CC_TGCA'GG Cuts at: 2301 2978 2301 Size: 677 4949 ScaI AGT'ACT Cuts at: 3874 5586 3874 Size: 1712 3914 SfcI C'TryA_G Cuts at: 826 1045 2297 2432 2455 2563 2974 4109 4790 Size: 219 1252 135 23 108 411 1135 681 Cuts at: 4790 4981 826 Size: 191 1471 Fragments arranged by size: 1471 1252 1135 681 411 219 191 135 108 23 SfiI GGCCn_nnn'nGGCC Cuts at: 2081 2609 2081 Size: 528 5098 SfoI GGC'GCC Cuts at: 2101 2101 Size: 5626 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5626 SmaI CCC'GGG Cuts at: 1872 2120 2953 1872 Size: 248 833 4545 Fragments arranged by size: 4545 833 248 SmlI C'TyrA_G Cuts at: 820 1017 1943 3730 4631 4893 820 Size: 197 926 1787 901 262 1553 Fragments arranged by size: 1787 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3417 493 Size: 2924 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5626 SphI G_CATG'C Cuts at: 1132 2371 2984 1132 Size: 1239 613 3774 Fragments arranged by size: 3774 1239 613 SspI AAT'ATT Cuts at: 5 52 3550 5 Size: 47 3498 2081 Fragments arranged by size: 3498 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5626 StyI C'CwwG_G Cuts at: 513 1065 2004 2079 2262 3065 513 Size: 552 939 75 183 803 3074 Fragments arranged by size: 3074 939 803 552 183 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2870 3653 3670 3823 4008 4453 325 Size: 1255 1290 783 17 153 185 445 1498 Fragments arranged by size: 1498 1290 1255 783 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2706 3872 5584 Size: 274 80 33 51 1443 729 1166 1712 Cuts at: 5584 96 Size: 138 Fragments arranged by size: 1712 1443 1166 729 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5586 5618 5586 Size: 32 5594 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5626 XbaI T'CTAG_A Cuts at: 2962 2962 Size: 5626 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5626 XmaI C'CCGG_G Cuts at: 1870 2118 2951 1870 Size: 248 833 4545 Fragments arranged by size: 4545 833 248 XmnI GAAnn'nnTTC Cuts at: 2789 3755 2789 Size: 966 4660 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3434 276 Size: 53 83 186 936 1900 2468 Fragments arranged by size: 2468 1900 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PvuII RsrII SacII SanDI SexAI SrfI SwaI XcmI