(Linear) (Six-base) MAPSORT of: KIEE6288.seq Check: 8327 from: 1 to: 921 KIEE6288.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 431 545 687 921 Size: 431 114 142 234 Fragments arranged by size: 431 234 142 114 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 358 921 Size: 358 563 AflIII A'CryG_T Cuts at: 0 14 921 Size: 14 907 AleI CACnn'nnGTG Cuts at: 0 472 921 Size: 472 449 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 490 921 Size: 490 431 AlwNI CAG_nnn'CTG Cuts at: 0 357 921 Size: 357 564 ApaI G_GGCC'C Cuts at: 0 185 569 614 715 921 Size: 185 384 45 101 206 Fragments arranged by size: 384 206 185 101 45 AvaI C'yCGr_G Cuts at: 0 113 139 178 669 818 921 Size: 113 26 39 491 149 103 Fragments arranged by size: 491 149 113 103 39 26 BanI G'GyrC_C Cuts at: 0 94 921 Size: 94 827 BanII G_rGCy'C Cuts at: 0 179 185 410 569 614 715 921 Size: 179 6 225 159 45 101 206 Fragments arranged by size: 225 206 179 159 101 45 6 BbeI G_GCGC'C Cuts at: 0 98 921 Size: 98 823 BbsI GAAGACnn'nnnn_ Cuts at: 0 99 921 Size: 99 822 BglI GCCn_nnn'nGGC Cuts at: 0 67 76 118 604 921 Size: 67 9 42 486 317 Fragments arranged by size: 486 317 67 42 9 Bme1580I G_kGCm'C Cuts at: 0 185 479 569 614 715 921 Size: 185 294 90 45 101 206 Fragments arranged by size: 294 206 185 101 90 45 BmgBI CAC'GTC Cuts at: 0 620 921 Size: 620 301 BmrI ACTGGGnnnn_n' Cuts at: 0 82 921 Size: 82 839 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 175 706 921 Size: 175 531 215 Fragments arranged by size: 531 215 175 BsaAI yAC'GTr Cuts at: 0 693 921 Size: 693 228 BsaHI Gr'CG_yC Cuts at: 0 95 921 Size: 95 826 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 328 921 Size: 328 593 BseYI C'CCAG_C Cuts at: 0 190 357 395 708 921 Size: 190 167 38 313 213 Fragments arranged by size: 313 213 190 167 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 360 403 517 715 730 921 Size: 154 206 43 114 198 15 191 Fragments arranged by size: 206 198 191 154 114 43 15 BsiEI CG_ry'CG Cuts at: 0 608 921 Size: 608 313 BsiHKAI G_wGCw'C Cuts at: 0 179 410 816 921 Size: 179 231 406 105 Fragments arranged by size: 406 231 179 105 Bsp1286I G_dGCh'C Cuts at: 0 179 185 410 479 569 614 715 816 Size: 179 6 225 69 90 45 101 101 Cuts at: 816 921 Size: 105 Fragments arranged by size: 225 179 105 101 101 90 69 45 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 218 425 431 545 687 921 Size: 218 207 6 114 142 234 Fragments arranged by size: 234 218 207 142 114 6 BsrFI r'CCGG_y Cuts at: 0 828 921 Size: 828 93 BstAPI GCAn_nnn'nTGC Cuts at: 0 426 921 Size: 426 495 BstEII G'GTnAC_C Cuts at: 0 252 585 921 Size: 252 333 336 Fragments arranged by size: 336 333 252 Bsu36I CC'TnA_GG Cuts at: 0 840 921 Size: 840 81 BtgI C'CryG_G Cuts at: 0 480 921 Size: 480 441 BtsI GCAGTG_nn' Cuts at: 0 900 921 Size: 900 21 EaeI y'GGCC_r Cuts at: 0 272 308 605 644 792 921 Size: 272 36 297 39 148 129 Fragments arranged by size: 297 272 148 129 39 36 EagI C'GGCC_G Cuts at: 0 605 921 Size: 605 316 EarI CTCTTCn'nnn_ Cuts at: 0 232 241 921 Size: 232 9 680 Fragments arranged by size: 680 232 9 EcoICRI GAG'CTC Cuts at: 0 177 408 921 Size: 177 231 513 Fragments arranged by size: 513 231 177 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 175 358 706 921 Size: 175 183 348 215 Fragments arranged by size: 348 215 183 175 EcoNI CCTnn'n_nnAGG Cuts at: 0 52 719 921 Size: 52 667 202 Fragments arranged by size: 667 202 52 EcoO109I rG'GnC_Cy Cuts at: 0 78 143 182 266 611 712 921 Size: 78 65 39 84 345 101 209 Fragments arranged by size: 345 209 101 84 78 65 39 FspI TGC'GCA Cuts at: 0 466 921 Size: 466 455 HaeII r_GCGC'y Cuts at: 0 98 490 921 Size: 98 392 431 Fragments arranged by size: 431 392 98 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 253 285 921 Size: 253 32 636 Fragments arranged by size: 636 253 32 KasI G'GCGC_C Cuts at: 0 94 921 Size: 94 827 MslI CAynn'nnrTG Cuts at: 0 472 921 Size: 472 449 MspA1I CmG'CkG Cuts at: 0 563 571 921 Size: 563 8 350 Fragments arranged by size: 563 350 8 NaeI GCC'GGC Cuts at: 0 830 921 Size: 830 91 NarI GG'CG_CC Cuts at: 0 95 921 Size: 95 826 NgoMIV G'CCGG_C Cuts at: 0 828 921 Size: 828 93 NspI r_CATG'y Cuts at: 0 366 921 Size: 366 555 PasI CC'CwG_GG Cuts at: 0 185 921 Size: 185 736 PfoI T'CCnGG_A Cuts at: 0 152 878 921 Size: 152 726 43 Fragments arranged by size: 726 152 43 PshAI GACnn'nnGTC Cuts at: 0 169 921 Size: 169 752 PspOMI G'GGCC_C Cuts at: 0 181 565 610 711 921 Size: 181 384 45 101 210 Fragments arranged by size: 384 210 181 101 45 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 772 804 921 Size: 772 32 117 Fragments arranged by size: 772 117 32 PstI C_TGCA'G Cuts at: 0 296 431 454 921 Size: 296 135 23 467 Fragments arranged by size: 467 296 135 23 SacI G_AGCT'C Cuts at: 0 179 410 921 Size: 179 231 511 Fragments arranged by size: 511 231 179 SapI GCTCTTCn'nnn_ Cuts at: 0 232 241 921 Size: 232 9 680 Fragments arranged by size: 680 232 9 SbfI CC_TGCA'GG Cuts at: 0 296 921 Size: 296 625 SfcI C'TryA_G Cuts at: 0 292 427 450 558 921 Size: 292 135 23 108 363 Fragments arranged by size: 363 292 135 108 23 SfiI GGCCn_nnn'nGGCC Cuts at: 0 76 604 921 Size: 76 528 317 Fragments arranged by size: 528 317 76 SfoI GGC'GCC Cuts at: 0 96 921 Size: 96 825 SmaI CCC'GGG Cuts at: 0 115 921 Size: 115 806 SphI G_CATG'C Cuts at: 0 366 921 Size: 366 555 StyI C'CwwG_G Cuts at: 0 74 257 921 Size: 74 183 664 Fragments arranged by size: 664 183 74 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 865 921 Size: 865 56 TatI w'GTAC_w Cuts at: 0 701 921 Size: 701 220 XmaI C'CCGG_G Cuts at: 0 113 921 Size: 113 808 XmnI GAAnn'nnTTC Cuts at: 0 784 921 Size: 784 137 Enzymes that do cut: AarI AcuI AflIII AleI AlfI AlwNI ApaI AvaI BanI BanII BbeI BbsI BglI Bme1580I BmgBI BmrI BpmI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstEII Bsu36I BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI MslI MspA1I NaeI NarI NgoMIV NspI PasI PfoI PshAI PspOMI PsrI PstI SacI SapI SbfI SfcI SfiI SfoI SmaI SphI StyI TaqII TatI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EciI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PpuMI PsiI PspXI PvuI PvuII RsrII SacII SalI SanDI ScaI SexAI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI ZraI