DDX28 ORF clone pFN21AE5694

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE5694

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Clone info.

Species

human

Clone Name

pFN21AE5694 Sequence (with vector) Restriction Map

Product ID

FHC16520

ORF Sequence

DNA sequence (1620 bp)
Protein sequence (540 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5694

Symbol

DDX28

Alias : MDDX28

Description

DEAD (Asp-Glu-Ala-Asp) box polypeptide 28

Original Clone ID

cp95694

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1620 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 540 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10858.1 3.3e-196 100.0 DDX28 CCDS8770.1 4.3e-27 29.0 DDX23 CCDS9918.1 1e-14 29.3 DDX24 CCDS32704.1 4.8e-14 30.0 DDX42 CCDS34240.1 1.3e-13 28.6 DDX46 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR011545 152 337 PF00270 DNA/RNA helicase IPR001650 418 492 PF00271 Helicase HMMSmart IPR014001 147 368 SM00487 DEAD-like helicase IPR001650 411 492 SM00490 Helicase ProfileScan IPR014014 126 156 PS51195 RNA helicase IPR014001 159 351 PS51192 DEAD-like helicase IPR001650 377 536 PS51194 Helicase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_060850 540 607618 100.0 100.0 NP_057439 455 615428 34.6 80.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5694 Download

>KIEE5694  1620 bp
ATGGCTCTAACGCGGCCGGTGCGGCTCTTTTCCCTCGTGACTCGGTTGCTCCTGGCGCCG
CGACGGGGCCTCACGGTCCGCAGTCCCGACGAACCCCTGCCGGTGGTGCGCATCCCAGTG
GCTCTACAGCGGCAGTTGGAACAGCGGCAGAGCAGGCGGCGGAACCTCCCGAGGCCGGTG
CTGGTTCGACCCGGACCGCTGCTGGTTTCGGCGCGGCGGCCGGAGTTGAACCAGCCGGCG
CGCCTCACACTGGGCCGTTGGGAGCGCGCGCCGCTAGCCTCTCAAGGCTGGAAGAGTCGA
CGCGCGCGTCGGGACCACTTCTCCATCGAGCGCGCGCAACAGGAGGCGCCAGCGGTGCGA
AAGCTCTCGTCTAAGGGCAGCTTTGCTGACCTGGGCCTGGAGCCCCGTGTGCTGCACGCA
CTACAGGAGGCTGCGCCTGAAGTCGTTCAGCCCACAACCGTGCAGTCTAGCACCATCCCC
TCACTACTTCGCGGCCGCCATGTCGTTTGCGCCGCAGAAACCGGCAGTGGCAAGACTCTC
AGCTACCTCCTGCCGCTGCTTCAACGGCTCTTGGGCCAGCCAAGCCTGGACTCCCTTCCT
ATCCCCGCGCCCCGAGGCCTGGTCCTTGTTCCTTCCCGAGAATTGGCCCAACAGGTGCGG
GCTGTGGCCCAACCCTTGGGCCGCTCCTTGGGCCTGCTGGTGCGGGACCTGGAGGGAGGC
CACGGCATGCGTAGGATCAGGCTGCAGCTGTCCAGACAGCCTTCAGCAGATGTGCTTGTG
GCCACTCCAGGGGCTCTGTGGAAGGCCCTGAAAAGTCGACTGATCAGTCTGGAGCAACTC
TCCTTCTTGGTGTTGGATGAGGCAGACACACTGCTGGATGAAAGCTTCCTGGAACTGGTG
GACTACATCTTAGAGAAGAGCCACATAGCAGAAGGCCCAGCTGACTTGGAAGACCCCTTC
AATCCCAAAGCTCAGTTAGTGCTGGTAGGAGCCACATTTCCCGAAGGTGTAGGCCAGTTG
CTGAATAAAGTCGCCAGCCCAGATGCTGTCACCACCATCACCAGCTCCAAGCTCCACTGT
ATCATGCCTCATGTGAAACAGACATTTCTGAGACTGAAGGGAGCAGATAAGGTGGCCGAG
CTGGTGCACATCCTCAAGCATCGTGACAGAGCAGAAAGGACTGGTCCCTCAGGAACTGTT
CTGGTGTTCTGTAATAGCTCCAGCACTGTGAACTGGCTGGGATATATTCTGGATGACCAC
AAAATCCAACACCTAAGGTTGCAGGGGCAAATGCCAGCCTTGATGAGGGTAGGAATCTTC
CAGTCCTTCCAGAAGAGCTCCCGAGACATACTTCTCTGCACAGACATAGCCTCTCGGGGC
CTGGACAGCACTGGTGTGGAGCTGGTTGTCAATTATGATTTCCCCCCAACGCTGCAAGAT
TACATCCACAGAGCAGGGAGAGTGGGCCGTGTGGGGAGCGAGGTGCCAGGCACCGTCATC
AGTTTTGTGACCCATCCCTGGGATGTGAGCCTGGTTCAGAAGATTGAGCTGGCGGCTCGC
CGAAGGAGAAGTCTTCCAGGACTAGCATCCTCGGTGAAAGAGCCTTTGCCCCAAGCAACC

ORF Protein

Cloned ORF protein sequence for pFN21AE5694 Download

>KIEE5694  540 aa
MALTRPVRLFSLVTRLLLAPRRGLTVRSPDEPLPVVRIPVALQRQLEQRQSRRRNLPRPV
LVRPGPLLVSARRPELNQPARLTLGRWERAPLASQGWKSRRARRDHFSIERAQQEAPAVR
KLSSKGSFADLGLEPRVLHALQEAAPEVVQPTTVQSSTIPSLLRGRHVVCAAETGSGKTL
SYLLPLLQRLLGQPSLDSLPIPAPRGLVLVPSRELAQQVRAVAQPLGRSLGLLVRDLEGG
HGMRRIRLQLSRQPSADVLVATPGALWKALKSRLISLEQLSFLVLDEADTLLDESFLELV
DYILEKSHIAEGPADLEDPFNPKAQLVLVGATFPEGVGQLLNKVASPDAVTTITSSKLHC
IMPHVKQTFLRLKGADKVAELVHILKHRDRAERTGPSGTVLVFCNSSSTVNWLGYILDDH
KIQHLRLQGQMPALMRVGIFQSFQKSSRDILLCTDIASRGLDSTGVELVVNYDFPPTLQD
YIHRAGRVGRVGSEVPGTVISFVTHPWDVSLVQKIELAARRRRSLPGLASSVKEPLPQAT

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5694 Download

>pFN21AE5694  6325 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTCTAACGCGGCCGGTGCGGCTCTTTTCCCT
CGTGACTCGGTTGCTCCTGGCGCCGCGACGGGGCCTCACGGTCCGCAGTCCCGACGAACC
CCTGCCGGTGGTGCGCATCCCAGTGGCTCTACAGCGGCAGTTGGAACAGCGGCAGAGCAG
GCGGCGGAACCTCCCGAGGCCGGTGCTGGTTCGACCCGGACCGCTGCTGGTTTCGGCGCG
GCGGCCGGAGTTGAACCAGCCGGCGCGCCTCACACTGGGCCGTTGGGAGCGCGCGCCGCT
AGCCTCTCAAGGCTGGAAGAGTCGACGCGCGCGTCGGGACCACTTCTCCATCGAGCGCGC
GCAACAGGAGGCGCCAGCGGTGCGAAAGCTCTCGTCTAAGGGCAGCTTTGCTGACCTGGG
CCTGGAGCCCCGTGTGCTGCACGCACTACAGGAGGCTGCGCCTGAAGTCGTTCAGCCCAC
AACCGTGCAGTCTAGCACCATCCCCTCACTACTTCGCGGCCGCCATGTCGTTTGCGCCGC
AGAAACCGGCAGTGGCAAGACTCTCAGCTACCTCCTGCCGCTGCTTCAACGGCTCTTGGG
CCAGCCAAGCCTGGACTCCCTTCCTATCCCCGCGCCCCGAGGCCTGGTCCTTGTTCCTTC
CCGAGAATTGGCCCAACAGGTGCGGGCTGTGGCCCAACCCTTGGGCCGCTCCTTGGGCCT
GCTGGTGCGGGACCTGGAGGGAGGCCACGGCATGCGTAGGATCAGGCTGCAGCTGTCCAG
ACAGCCTTCAGCAGATGTGCTTGTGGCCACTCCAGGGGCTCTGTGGAAGGCCCTGAAAAG
TCGACTGATCAGTCTGGAGCAACTCTCCTTCTTGGTGTTGGATGAGGCAGACACACTGCT
GGATGAAAGCTTCCTGGAACTGGTGGACTACATCTTAGAGAAGAGCCACATAGCAGAAGG
CCCAGCTGACTTGGAAGACCCCTTCAATCCCAAAGCTCAGTTAGTGCTGGTAGGAGCCAC
ATTTCCCGAAGGTGTAGGCCAGTTGCTGAATAAAGTCGCCAGCCCAGATGCTGTCACCAC
CATCACCAGCTCCAAGCTCCACTGTATCATGCCTCATGTGAAACAGACATTTCTGAGACT
GAAGGGAGCAGATAAGGTGGCCGAGCTGGTGCACATCCTCAAGCATCGTGACAGAGCAGA
AAGGACTGGTCCCTCAGGAACTGTTCTGGTGTTCTGTAATAGCTCCAGCACTGTGAACTG
GCTGGGATATATTCTGGATGACCACAAAATCCAACACCTAAGGTTGCAGGGGCAAATGCC
AGCCTTGATGAGGGTAGGAATCTTCCAGTCCTTCCAGAAGAGCTCCCGAGACATACTTCT
CTGCACAGACATAGCCTCTCGGGGCCTGGACAGCACTGGTGTGGAGCTGGTTGTCAATTA
TGATTTCCCCCCAACGCTGCAAGATTACATCCACAGAGCAGGGAGAGTGGGCCGTGTGGG
GAGCGAGGTGCCAGGCACCGTCATCAGTTTTGTGACCCATCCCTGGGATGTGAGCCTGGT
TCAGAAGATTGAGCTGGCGGCTCGCCGAAGGAGAAGTCTTCCAGGACTAGCATCCTCGGT
GAAAGAGCCTTTGCCCCAAGCAACCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT