(Circular) (Six-base) MAPSORT of: pFN21AE5694.seq Check: 3711 from: 1 to: 6325 pFN21AE5694 6325 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4135 278 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2302 2821 3668 1937 Size: 365 519 847 4594 Fragments arranged by size: 4594 847 519 365 Acc65I G'GTAC_C Cuts at: 3646 3646 Size: 6325 AclI AA'CG_TT Cuts at: 1563 4452 4825 1563 Size: 2889 373 3063 Fragments arranged by size: 3063 2889 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2463 2751 3140 4388 5772 6040 1632 Size: 831 288 389 1248 1384 268 1917 Fragments arranged by size: 1917 1384 1248 831 389 288 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6128 AflIII A'CryG_T Cuts at: 1203 5224 6201 1203 Size: 4021 977 1327 Fragments arranged by size: 4021 1327 977 AgeI A'CCGG_T Cuts at: 5156 5156 Size: 6325 AhdI GACnn_n'nnGTC Cuts at: 5054 5054 Size: 6325 AleI CACnn'nnGTG Cuts at: 1166 2462 3398 1166 Size: 1296 936 4093 Fragments arranged by size: 4093 1296 936 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6325 AlwNI CAG_nnn'CTG Cuts at: 3025 3050 3200 4068 5640 3025 Size: 25 150 868 1572 3710 Fragments arranged by size: 3710 1572 868 150 25 ApaLI G'TGCA_C Cuts at: 3149 4382 5538 3149 Size: 1233 1156 3936 Fragments arranged by size: 3936 1233 1156 ApoI r'AATT_y Cuts at: 1493 3634 3860 4012 6121 1493 Size: 2141 226 152 2109 1697 Fragments arranged by size: 2141 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 2243 2243 Size: 6325 AseI AT'TA_AT Cuts at: 160 1032 4879 160 Size: 872 3847 1606 Fragments arranged by size: 3847 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 AvaI C'yCGr_G Cuts at: 1870 1943 2173 2616 2640 3345 3378 3650 1870 Size: 73 230 443 24 705 33 272 4545 Fragments arranged by size: 4545 705 443 272 230 73 33 24 BamHI G'GATC_C Cuts at: 3655 4022 3655 Size: 367 5958 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2059 2350 3487 3494 3646 Size: 325 217 627 272 291 1137 7 152 Cuts at: 3646 5101 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1137 627 325 291 272 217 152 7 BanII G_rGCy'C Cuts at: 1391 2409 2800 3344 3644 1391 Size: 1018 391 544 300 4072 Fragments arranged by size: 4072 1018 544 391 300 BbeI G_GCGC'C Cuts at: 2063 2354 2063 Size: 291 6034 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2961 3569 928 Size: 971 1062 608 3684 Fragments arranged by size: 3684 1062 971 608 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2164 2198 4516 4550 1903 Size: 34 227 34 2318 34 3678 Fragments arranged by size: 3678 2318 227 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4217 5427 4217 Size: 1210 5115 BclI T'GATC_A Cuts at: 1548 2826 1548 Size: 1278 5047 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2026 4936 136 Size: 107 122 71 631 605 354 2910 1525 Fragments arranged by size: 2910 1525 631 605 354 122 107 71 BglII A'GATC_T Cuts at: 6320 6320 Size: 6325 BlpI GC'TnA_GC Cuts at: 3742 3742 Size: 6325 Bme1580I G_kGCm'C Cuts at: 1790 3153 4386 5542 1790 Size: 1363 1233 1156 2573 Fragments arranged by size: 2573 1363 1233 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6325 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2114 2264 4056 Size: 416 53 83 399 24 697 150 1792 Cuts at: 4056 5014 442 Size: 958 1753 Fragments arranged by size: 1792 1753 958 697 416 399 150 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2282 1056 Size: 1226 5099 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2860 2860 Size: 6325 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2423 2735 2775 2855 3208 4985 1239 Size: 1184 312 40 80 353 1777 2579 Fragments arranged by size: 2579 1777 1184 353 312 80 40 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2271 3143 4450 5315 5613 2271 Size: 872 1307 865 298 2983 Fragments arranged by size: 2983 1307 872 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4988 882 Size: 617 3489 2219 Fragments arranged by size: 3489 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4116 493 Size: 711 2912 2702 Fragments arranged by size: 2912 2702 711 BsaBI GATnn'nnATC Cuts at: 6319 6319 Size: 6325 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2060 2351 4132 4514 Size: 53 83 186 936 527 291 1781 382 Cuts at: 4514 5149 275 Size: 635 1451 Fragments arranged by size: 1781 1451 936 635 527 382 291 186 83 53 BsaWI w'CCGG_w Cuts at: 4758 5156 5430 5577 5988 4758 Size: 398 274 147 411 5095 Fragments arranged by size: 5095 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2309 2339 1178 Size: 30 165 30 906 30 5164 Fragments arranged by size: 5164 906 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6325 BseYI C'CCAG_C Cuts at: 2941 3241 5528 6024 2941 Size: 300 2287 496 3242 Fragments arranged by size: 3242 2287 496 300 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2402 2486 3346 1163 Size: 573 666 84 860 4142 Fragments arranged by size: 4142 860 666 573 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2021 2225 2500 3775 4536 5564 Size: 275 186 20 204 275 1275 761 1028 Cuts at: 5564 1540 Size: 2301 Fragments arranged by size: 2301 1275 1028 761 275 275 204 186 20 BsiHKAI G_wGCw'C Cuts at: 3153 3344 4386 4471 5542 3153 Size: 191 1042 85 1071 3936 Fragments arranged by size: 3936 1071 1042 191 85 BsmI GAATG_Cn' Cuts at: 3836 3929 3836 Size: 93 6232 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2409 2800 3153 3344 3644 4386 4471 Size: 399 619 391 353 191 300 742 85 Cuts at: 4471 5542 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 619 399 391 353 300 191 85 BspEI T'CCGG_A Cuts at: 5988 5988 Size: 6325 BspHI T'CATG_A Cuts at: 4214 5944 4214 Size: 1730 4595 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3680 844 Size: 2836 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2688 4212 1394 Size: 429 865 1524 3507 Fragments arranged by size: 3507 1524 865 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4820 4994 65 Size: 1183 3572 174 1396 Fragments arranged by size: 3572 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2020 2104 2179 2239 2525 4969 5156 Size: 110 210 84 75 60 286 2444 187 Cuts at: 5156 1700 Size: 2869 Fragments arranged by size: 2869 2444 286 210 187 110 84 75 60 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6325 BssHII G'CGCG_C Cuts at: 1925 2243 2269 2271 2307 2335 2337 1925 Size: 318 26 2 36 28 2 5913 Fragments arranged by size: 5913 318 36 28 26 2 2 BssSI C'ACGA_G Cuts at: 2039 4385 5397 2039 Size: 2346 1012 2967 Fragments arranged by size: 2967 2346 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6325 BstBI TT'CG_AA Cuts at: 5129 5129 Size: 6325 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6325 BstYI r'GATC_y Cuts at: 1972 3655 4022 4407 4424 5876 5962 5974 6320 Size: 1683 367 385 17 1452 86 12 346 Cuts at: 6320 1972 Size: 1977 Fragments arranged by size: 1977 1683 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3193 3278 3193 Size: 85 6240 BtgI C'CryG_G Cuts at: 513 1065 2004 2725 513 Size: 552 939 721 4113 Fragments arranged by size: 4113 939 721 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5586 Fragments arranged by size: 5586 683 56 BtsI GCAGTG_nn' Cuts at: 2536 2873 3841 4635 4655 2536 Size: 337 968 794 20 4206 Fragments arranged by size: 4206 968 794 337 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6325 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6290 DraI TTT'AAA Cuts at: 3629 3629 Size: 6325 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2310 2829 5332 809 Size: 507 994 519 2503 1802 Fragments arranged by size: 2503 1802 994 519 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2018 2222 2497 Size: 54 1425 50 275 18 188 204 275 Cuts at: 2497 2784 3138 3772 4661 6290 8 Size: 287 354 634 889 1629 43 Fragments arranged by size: 1629 1425 889 634 354 287 275 275 204 188 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2018 2222 2497 3772 1537 Size: 275 206 204 275 1275 4090 Fragments arranged by size: 4090 1275 275 275 206 204 EarI CTCTTCn'nnn_ Cuts at: 1353 2291 2915 3332 4255 1353 Size: 938 624 417 923 3423 Fragments arranged by size: 3423 938 923 624 417 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2179 4912 5286 5432 387 Size: 1064 138 590 2733 374 146 1280 Fragments arranged by size: 2733 1280 1064 590 374 146 138 EcoICRI GAG'CTC Cuts at: 3342 3342 Size: 6325 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2423 2463 2735 2751 2775 2855 3140 Size: 393 791 40 272 16 24 80 285 Cuts at: 3140 3208 4388 4985 5772 6040 1239 Size: 68 1180 597 787 268 1524 Fragments arranged by size: 1524 1180 791 787 597 393 285 272 268 80 68 40 24 16 EcoNI CCTnn'n_nnAGG Cuts at: 3307 3307 Size: 6325 EcoO109I rG'GnC_Cy Cuts at: 2071 2710 2809 3382 2071 Size: 639 99 573 5014 Fragments arranged by size: 5014 639 573 99 EcoRI G'AATT_C Cuts at: 3634 4012 3634 Size: 378 5947 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2401 2401 Size: 6325 FspI TGC'GCA Cuts at: 2114 4831 2114 Size: 2717 3608 FspAI rTGC'GCAy Cuts at: 2114 2114 Size: 6325 HaeII r_GCGC'y Cuts at: 1678 2063 2354 5472 1678 Size: 385 291 3118 2531 Fragments arranged by size: 3118 2531 385 291 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2058 2090 2309 2341 2610 Size: 32 135 32 486 32 219 32 269 Cuts at: 2610 2642 3359 3391 4966 4998 5040 5072 1373 Size: 32 717 32 1575 32 42 32 2626 Fragments arranged by size: 2626 1575 717 486 269 219 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2303 2822 3669 3915 669 Size: 988 281 365 519 847 246 3079 Fragments arranged by size: 3079 988 847 519 365 281 246 HindIII A'AGCT_T Cuts at: 748 2887 748 Size: 2139 4186 HpaI GTT'AAC Cuts at: 3915 3915 Size: 6325 KasI G'GCGC_C Cuts at: 2059 2350 2059 Size: 291 6034 KpnI G_GTAC'C Cuts at: 3650 3650 Size: 6325 MfeI C'AATT_G Cuts at: 3924 3924 Size: 6325 MluI A'CGCG_T Cuts at: 6201 6201 Size: 6325 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2121 2838 3295 5439 5623 1305 Size: 816 717 457 2144 184 2007 Fragments arranged by size: 2144 2007 816 717 457 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2786 10 Size: 54 1425 343 954 3549 Fragments arranged by size: 3549 1425 954 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2120 2462 3398 4283 4642 4801 518 Size: 648 954 342 936 885 359 159 2042 Fragments arranged by size: 2042 954 936 885 648 359 342 159 MspA1I CmG'CkG Cuts at: 1606 2134 2149 2203 2357 2560 2752 2945 3741 Size: 528 15 54 154 203 192 193 796 Cuts at: 3741 4418 5566 5811 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 796 677 528 245 203 193 192 154 54 15 NaeI GCC'GGC Cuts at: 2241 2241 Size: 6325 NarI GG'CG_CC Cuts at: 2060 2351 2060 Size: 291 6034 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4834 Fragments arranged by size: 4834 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6325 NgoMIV G'CCGG_C Cuts at: 2239 2239 Size: 6325 NheI G'CTAG_C Cuts at: 1052 2278 1052 Size: 1226 5099 NotI GC'GGCC_GC Cuts at: 2497 3772 2497 Size: 1275 5050 NspI r_CATG'y Cuts at: 1132 1229 2734 3683 5228 1132 Size: 97 1505 949 1545 2229 Fragments arranged by size: 2229 1545 1505 949 97 PasI CC'CwG_GG Cuts at: 1348 3522 1348 Size: 2174 4151 PciI A'CATG_T Cuts at: 5224 5224 Size: 6325 PflMI CCAn_nnn'nTGG Cuts at: 1108 2680 1108 Size: 1572 4753 PfoI T'CCnGG_A Cuts at: 2892 3580 2892 Size: 688 5637 PmeI GTTT'AAAC Cuts at: 3629 3629 Size: 6325 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4394 4426 5907 5939 4394 Size: 32 1481 32 4780 Fragments arranged by size: 4780 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2710 2710 Size: 6325 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6325 PsiI TTA'TAA Cuts at: 3895 3895 Size: 6325 PstI C_TGCA'G Cuts at: 830 2751 3677 830 Size: 1921 926 3478 Fragments arranged by size: 3478 1921 926 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6325 PvuII CAG'CTG Cuts at: 2752 2945 2752 Size: 193 6132 RsrII CG'GwC_CG Cuts at: 2080 2198 2080 Size: 118 6207 SacI G_AGCT'C Cuts at: 3344 3344 Size: 6325 SalI G'TCGA_C Cuts at: 1936 2301 2820 3667 1936 Size: 365 519 847 4594 Fragments arranged by size: 4594 847 519 365 SapI GCTCTTCn'nnn_ Cuts at: 2915 3332 2915 Size: 417 5908 SbfI CC_TGCA'GG Cuts at: 3677 3677 Size: 6325 ScaI AGT'ACT Cuts at: 4573 6285 4573 Size: 1712 4613 SfcI C'TryA_G Cuts at: 826 1045 2129 2426 2747 3673 4808 5489 5680 Size: 219 1084 297 321 926 1135 681 191 Cuts at: 5680 826 Size: 1471 Fragments arranged by size: 1471 1135 1084 926 681 321 297 219 191 SfoI GGC'GCC Cuts at: 2061 2352 2061 Size: 291 6034 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6325 SmaI CCC'GGG Cuts at: 1872 3652 1872 Size: 1780 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2286 3158 4429 5330 5592 820 Size: 197 926 343 872 1271 901 262 1553 Fragments arranged by size: 1553 1271 926 901 872 343 262 197 SnaBI TAC'GTA Cuts at: 493 4116 493 Size: 3623 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6325 SphI G_CATG'C Cuts at: 1132 2734 3683 1132 Size: 1602 949 3774 Fragments arranged by size: 3774 1602 949 SspI AAT'ATT Cuts at: 5 52 4249 5 Size: 47 4197 2081 Fragments arranged by size: 4197 2081 47 StuI AGG'CCT Cuts at: 1517 2622 1517 Size: 1105 5220 StyI C'CwwG_G Cuts at: 513 1065 2004 2679 2691 3764 513 Size: 552 939 675 12 1073 3074 Fragments arranged by size: 3074 1073 939 675 552 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4352 4369 4522 4707 5152 325 Size: 1255 2772 17 153 185 445 1498 Fragments arranged by size: 2772 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4571 6283 96 Size: 274 80 33 51 1443 2594 1712 138 Fragments arranged by size: 2594 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6285 6317 6285 Size: 32 6293 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6325 XbaI T'CTAG_A Cuts at: 3661 3661 Size: 6325 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2799 2799 Size: 6325 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6325 XmaI C'CCGG_G Cuts at: 1870 3650 1870 Size: 1780 4545 XmnI GAAnn'nnTTC Cuts at: 2448 4454 2448 Size: 2006 4319 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4133 276 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI NruI NsiI PacI PmlI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SrfI SwaI