(Linear) (Six-base) MAPSORT of: KIEE5694.seq Check: 4529 from: 1 to: 1620 KIEE5694.orf With 182 enzymes: SgfI * December 5, 2014 13:07 .. AccI GT'mk_AC Cuts at: 0 297 816 1620 Size: 297 519 804 Fragments arranged by size: 804 519 297 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 458 746 1135 1620 Size: 458 288 389 485 Fragments arranged by size: 485 458 389 288 AleI CACnn'nnGTG Cuts at: 0 457 1393 1620 Size: 457 936 227 Fragments arranged by size: 936 457 227 AlwNI CAG_nnn'CTG Cuts at: 0 1020 1045 1195 1620 Size: 1020 25 150 425 Fragments arranged by size: 1020 425 150 25 ApaLI G'TGCA_C Cuts at: 0 1144 1620 Size: 1144 476 AscI GG'CGCG_CC Cuts at: 0 238 1620 Size: 238 1382 AvaI C'yCGr_G Cuts at: 0 168 611 635 1340 1373 1620 Size: 168 443 24 705 33 247 Fragments arranged by size: 705 443 247 168 33 24 BanI G'GyrC_C Cuts at: 0 54 345 1482 1489 1620 Size: 54 291 1137 7 131 Fragments arranged by size: 1137 291 131 54 7 BanII G_rGCy'C Cuts at: 0 404 795 1339 1620 Size: 404 391 544 281 Fragments arranged by size: 544 404 391 281 BbeI G_GCGC'C Cuts at: 0 58 349 1620 Size: 58 291 1271 Fragments arranged by size: 1271 291 58 BbsI GAAGACnn'nnnn_ Cuts at: 0 956 1564 1620 Size: 956 608 56 Fragments arranged by size: 956 608 56 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 159 193 1620 Size: 159 34 1427 Fragments arranged by size: 1427 159 34 BclI T'GATC_A Cuts at: 0 821 1620 Size: 821 799 BglI GCCn_nnn'nGGC Cuts at: 0 21 1620 Size: 21 1599 Bme1580I G_kGCm'C Cuts at: 0 1148 1620 Size: 1148 472 BmrI ACTGGGnnnn_n' Cuts at: 0 109 259 1620 Size: 109 150 1361 Fragments arranged by size: 1361 150 109 BmtI G_CTAG'C Cuts at: 0 277 1620 Size: 277 1343 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 855 1620 Size: 855 765 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 418 730 770 850 1203 1620 Size: 418 312 40 80 353 417 Fragments arranged by size: 418 417 353 312 80 40 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 266 1138 1620 Size: 266 872 482 Fragments arranged by size: 872 482 266 BsaHI Gr'CG_yC Cuts at: 0 55 346 1620 Size: 55 291 1274 Fragments arranged by size: 1274 291 55 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 304 334 1620 Size: 304 30 1286 Fragments arranged by size: 1286 304 30 BseYI C'CCAG_C Cuts at: 0 936 1236 1620 Size: 936 300 384 Fragments arranged by size: 936 384 300 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 397 481 1341 1620 Size: 397 84 860 279 Fragments arranged by size: 860 397 279 84 BsiEI CG_ry'CG Cuts at: 0 16 220 495 1620 Size: 16 204 275 1125 Fragments arranged by size: 1125 275 204 16 BsiHKAI G_wGCw'C Cuts at: 0 1148 1339 1620 Size: 1148 191 281 Fragments arranged by size: 1148 281 191 Bsp1286I G_dGCh'C Cuts at: 0 404 795 1148 1339 1620 Size: 404 391 353 191 281 Fragments arranged by size: 404 391 353 281 191 BsrBI CCG'CTC Cuts at: 0 683 1620 Size: 683 937 BsrFI r'CCGG_y Cuts at: 0 15 99 174 234 520 1620 Size: 15 84 75 60 286 1100 Fragments arranged by size: 1100 286 84 75 60 15 BssHII G'CGCG_C Cuts at: 0 238 264 266 302 330 332 1620 Size: 238 26 2 36 28 2 1288 Fragments arranged by size: 1288 238 36 28 26 2 2 BssSI C'ACGA_G Cuts at: 0 34 1620 Size: 34 1586 Bsu36I CC'TnA_GG Cuts at: 0 1188 1273 1620 Size: 1188 85 347 Fragments arranged by size: 1188 347 85 BtgI C'CryG_G Cuts at: 0 720 1620 Size: 720 900 BtsI GCAGTG_nn' Cuts at: 0 531 868 1620 Size: 531 337 752 Fragments arranged by size: 752 531 337 DrdI GACnn_nn'nnGTC Cuts at: 0 305 824 1620 Size: 305 519 796 Fragments arranged by size: 796 519 305 EaeI y'GGCC_r Cuts at: 0 13 217 492 779 1133 1620 Size: 13 204 275 287 354 487 Fragments arranged by size: 487 354 287 275 204 13 EagI C'GGCC_G Cuts at: 0 13 217 492 1620 Size: 13 204 275 1128 Fragments arranged by size: 1128 275 204 13 EarI CTCTTCn'nnn_ Cuts at: 0 286 910 1327 1620 Size: 286 624 417 293 Fragments arranged by size: 624 417 293 286 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 174 1620 Size: 174 1446 EcoICRI GAG'CTC Cuts at: 0 1337 1620 Size: 1337 283 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 418 458 730 746 770 850 1135 1203 Size: 418 40 272 16 24 80 285 68 Cuts at: 1203 1620 Size: 417 Fragments arranged by size: 418 417 285 272 80 68 40 24 16 EcoNI CCTnn'n_nnAGG Cuts at: 0 1302 1620 Size: 1302 318 EcoO109I rG'GnC_Cy Cuts at: 0 66 705 804 1377 1620 Size: 66 639 99 573 243 Fragments arranged by size: 639 573 243 99 66 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 396 1620 Size: 396 1224 FspI TGC'GCA Cuts at: 0 109 1620 Size: 109 1511 FspAI rTGC'GCAy Cuts at: 0 109 1620 Size: 109 1511 HaeII r_GCGC'y Cuts at: 0 58 349 1620 Size: 58 291 1271 Fragments arranged by size: 1271 291 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 53 85 304 336 605 637 1354 1386 Size: 53 32 219 32 269 32 717 32 Cuts at: 1386 1620 Size: 234 Fragments arranged by size: 717 269 234 219 53 32 32 32 32 HincII GTy'rAC Cuts at: 0 298 817 1620 Size: 298 519 803 Fragments arranged by size: 803 519 298 HindIII A'AGCT_T Cuts at: 0 882 1620 Size: 882 738 KasI G'GCGC_C Cuts at: 0 54 345 1620 Size: 54 291 1275 Fragments arranged by size: 1275 291 54 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 116 833 1290 1620 Size: 116 717 457 330 Fragments arranged by size: 717 457 330 116 MscI TGG'CCA Cuts at: 0 781 1620 Size: 781 839 MslI CAynn'nnrTG Cuts at: 0 115 457 1393 1620 Size: 115 342 936 227 Fragments arranged by size: 936 342 227 115 MspA1I CmG'CkG Cuts at: 0 129 144 198 352 555 747 940 1620 Size: 129 15 54 154 203 192 193 680 Fragments arranged by size: 680 203 193 192 154 129 54 15 NaeI GCC'GGC Cuts at: 0 236 1620 Size: 236 1384 NarI GG'CG_CC Cuts at: 0 55 346 1620 Size: 55 291 1274 Fragments arranged by size: 1274 291 55 NgoMIV G'CCGG_C Cuts at: 0 234 1620 Size: 234 1386 NheI G'CTAG_C Cuts at: 0 273 1620 Size: 273 1347 NotI GC'GGCC_GC Cuts at: 0 492 1620 Size: 492 1128 NspI r_CATG'y Cuts at: 0 729 1620 Size: 729 891 PasI CC'CwG_GG Cuts at: 0 1517 1620 Size: 1517 103 PflMI CCAn_nnn'nTGG Cuts at: 0 675 1620 Size: 675 945 PfoI T'CCnGG_A Cuts at: 0 887 1575 1620 Size: 887 688 45 Fragments arranged by size: 887 688 45 PpuMI rG'GwC_Cy Cuts at: 0 705 1620 Size: 705 915 PstI C_TGCA'G Cuts at: 0 746 1620 Size: 746 874 PvuII CAG'CTG Cuts at: 0 747 940 1620 Size: 747 193 680 Fragments arranged by size: 747 680 193 RsrII CG'GwC_CG Cuts at: 0 75 193 1620 Size: 75 118 1427 Fragments arranged by size: 1427 118 75 SacI G_AGCT'C Cuts at: 0 1339 1620 Size: 1339 281 SalI G'TCGA_C Cuts at: 0 296 815 1620 Size: 296 519 805 Fragments arranged by size: 805 519 296 SapI GCTCTTCn'nnn_ Cuts at: 0 910 1327 1620 Size: 910 417 293 Fragments arranged by size: 910 417 293 SfcI C'TryA_G Cuts at: 0 124 421 742 1620 Size: 124 297 321 878 Fragments arranged by size: 878 321 297 124 SfoI GGC'GCC Cuts at: 0 56 347 1620 Size: 56 291 1273 Fragments arranged by size: 1273 291 56 SmlI C'TyrA_G Cuts at: 0 281 1153 1620 Size: 281 872 467 Fragments arranged by size: 872 467 281 SphI G_CATG'C Cuts at: 0 729 1620 Size: 729 891 StuI AGG'CCT Cuts at: 0 617 1620 Size: 617 1003 StyI C'CwwG_G Cuts at: 0 674 686 1620 Size: 674 12 934 Fragments arranged by size: 934 674 12 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 794 1620 Size: 794 826 XmnI GAAnn'nnTTC Cuts at: 0 443 1620 Size: 443 1177 Enzymes that do cut: AccI AcuI AleI AlwNI ApaLI AscI AvaI BanI BanII BbeI BbsI BcgI BclI BglI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BssHII BssSI Bsu36I BtgI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI NotI NspI PasI PflMI PfoI PpuMI PstI PvuII RsrII SacI SalI SapI SfcI SfoI SmlI SphI StuI StyI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApoI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BfrBI BglII BlpI BmgBI Bpu10I BsaI BsaAI BsaBI BsaWI BseRI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII EcoRI EcoRV FseI HpaI KpnI MfeI MluI NcoI NdeI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI SacII SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI ZraI